Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (8): 23-29.doi: 10.6040/j.issn.1671-7554.0.2022.0011

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Trillium Saponins ameliorates pulmonary fibrosis in rats by inhibiting TGF-β/Smad3 and Wnt/β-catenin signaling pathways

ZHANG Bingfen1, ZHOU Shenghong2, WANG Zhe3   

  1. 1. Department of Traditional Chinese Medicine Clinical, Shandong Academy of Traditional Chinese Medicine, Jinan 250014, Shandong, China;
    2. Department of Acupuncture and Medicine, Shandong Academy of Traditional Chinese Medicine, Jinan 250014, Shandong, China;
    3. Department of Traditional Chinese Medicine, Jinan Shizhong Peoples Hospital, Jinan 250002, Shandong, China
  • Published:2022-07-27

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Abstract: Objective To investigate the treatment and mechanism of Trillium Saponins in rats with pulmonary interstitial fibrosis. Methods Fifty male SD rats were divided into 5 groups, namely the control group, the model group, and the low, medium, and high Trillium Saponins treatment groups, with 10 in each group. Except the control group, the other groups were injected with bleomycin 5 mg/kg through tracheal intubation. A rat model of pulmonary fibrosis was established. The treatment group was given 25, 50 and 100 mg/(kg·d)Trillium Saponins by gavage the next day. After 28 days of treatment, the rats were sacrificed, and blood and lung tissues were taken. The lung tissue was weighed and the lung coefficient was calculated. HE staining and Masson staining were used to observe rat lung histopathology. The expression of interleukin-1β(IL-1β), tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), malondialdehyde(MDA)and reactive oxygen species(ROS)and the activity of superoxide dismutase(SOD)were detected by ELISA kit. Western blotting experiment was employed to detect the protein expression levels of transforming growth factor-β(TGF-β)1, Smad3, Wnt3a, β-catenin, glycogen synthase kinase-3β(GSK-3β), fibronectin, Collagen Ⅰ and α-SMA in lung tissue. Results Compared with the model group, lung coefficient was decreased, inflammatory cell infiltration, collagen deposition and fibrocytosis was reduced, the expression of IL-1β, TNF-α, IL-6, MDA and ROS levels was decrease, the activity of SOD was increased, the expression of fibronectin, Collagen Ⅰ, α-SMA, TGFβ1, Smad3, Wnt3a, β-catenin and GSK-3β proteins was inhibited in lung tissue of low, medium, and high Trillium Saponins treatment groups. Conclusion Trillium has anti-inflammatory and anti-oxidant properties, and can inhibit the activation of TGF-β/Smad3 and Wnt/β-catenin signaling pathways to protect against pulmonary fibrosis.

Key words: Trillium saponin, Pulmonary interstitial fibrosis, Inflammation, Transforming growth factor-β/Smad3 signaling pathway, Wnt/β-catenin signaling pathway

CLC Number: 

  • R285.5
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