Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (9): 17-22.doi: 10.6040/j.issn.1671-7554.0.2018.588

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Chemical constituents from hemp seeds and hemp flowers

ZHANG Li1, ZHOU Yuefang2, WANG Shanshan2, WU Xuyi2, LIANG Xiaomin2, YAN Xiaoli2, FAN Peihong2   

  1. 1. Department of Pharmacy, Jinan 2nd Peoples Hospital, Jinan 250001, Shandong, China;
    2. Department of Natural Product Chemistry, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To isolate and identify the chemical constituents from hemp seeds and hemp flowers, and study their bioactivities. Methods The chemical constituents were isolated and purified by macroporous adsorption resin, silica gel, octadecylsilyl, sephadex LH-20 column chromatography and HPLC. The structures of compounds were identified by 1HNMR, 13CNMR, HSQC, and HMBC spectral data. The anti-neuroinlammatory activity of the compounds were screened using lipopolysaccharide(LPS)stimulated BV2 microglia cell model, and the inflammatory factors in BV2 cells were measured using ELISA test. Results Five compounds isolated from the hemp seeds, and were identified as 1-monolinolein(1), 1-monopalmitin(2), 4-methoxy-3H-benzofuran-2-one(3),(E)-3-(4-hydroxyphenyl)acrylic acid ethyl ester(4)and polybotrin(5). Furthermore, five compounds were isolated from hemp flowers and identified as β-sitosterol(6), acacetin(7), cannflavin A(8), apigenin-7-O-β-D-glucuronide(9), and kaempferol-3-O-sophoroside(10). In addition, compounds 8-10 exhibited inhibitory effects on tumor necrosis factor α(TNF-α)released from lipopolysaccharide(LPS)-induced BV2 microglia cells. Conclusion Compounds 3 and 4 are obtained from hemp seed for the first time, and compounds 7, 9, and 10 are obtained from hemp flowers for the first time; compounds 8, 9, and 10 have potential anti-neuroinflammation effects.

Key words: Hemp seed, Hemp flower, Anti-neuroinflammation, Chemical constituents

CLC Number: 

  • R932
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