Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (6): 10-18.doi: 10.6040/j.issn.1671-7554.0.2022.0380

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Effects of Hanjingtang and its disassembled prescriptions on serum inflammatory factors, vascular endothelial function and fibrosis in hypertensive rats with cold coagulation syndrome

WU Yujie1, ZHANG Mingquan1, JI Yongli2, ZHAO Lu1, WANG Yue1, CHEN Shasha1   

  1. 1. The Basic Medical College of Hebei University of Chinese Medicine, Shijiazhuang 050200, Hebei, China;
    2. The B-US of Shengli Oilfield Central Hospital, Dongying 257034, Shandong, China
  • Published:2022-06-17

Abstract: Objective To observe and compare the antihypertensive effects of Hanjingtang and its disassembled prescriptions on hypertensive rats with cold coagulation syndrome and the influence on serum inflammatory factors, vascular endothelial function and vascular collagen fiber, so that to explore the antihypertensive mechanism. Methods A total of 40 male SD rats aged 6 to 8 weeks were randomly divided into control group, model group, Hanjingtang group, Tongyang group and Tongluo group, with 8 rats in each group. The control group was fed with ordinary diet, and the other groups were given diet with 8% sodium chloride and high salt, and placed in a freezer at(-15±2)℃ for 3 hours every day for 6 weeks, followed by intragastric administration for 2 weeks. The control group and model group were given equal volume of normal saline. The blood pressure was measured twice a week with the rat noninvasive blood pressure analysis system, and the average value was taken. The changes of aortic pathological morphology and collagen deposition were observed with HE and Masson staining. The IL-1β, TNF- α and ET-1 were detected with ELISA, and NO was detected with colorimetry. The expressions of eNOS, type I collagen(ColI)and TGF-β1 in aortic tissue were detected with IHC. Results (1) Compared with the control group, the model group had raised blood pressure(P<0.01); incomplete aortic intima; thicker intima-media; disordered cell arranging; increased serum TNF-α, IL-1β, IL-6 and ET-1; decreased NO; increased collagen fibers. Meanwhile, IHC showed that eNOS expression decreased, but ColI and TGF-β1 expressions increased in aorta(P<0.01). (2) Compared with the model group, the Hanjingtang, Tongyang and Tongluo groups had decreased blood pressure(P<0.01, P<0.05); improved aortic structure at different levels; increased serum NO(P<0.05); Hanjingtang group had decreased TNF-α, IL-1β, ET-1 and IL-6(P<0.01, P<0.05); Tongyang group had decreased TNF-α, IL-1β and IL-6(P<0.05); Tongluo group had decreased ET-1 and TNF-α(P<0.05). IHC showed that the expression of ColI in aorta decreased but eNOS increased in the three treatment groups(P<0.05). The expression of TGF-β1 decreased in Hanjingtang and Tongyang groups(P<0.01, P<0.05); aortic collagen fibers decreased in the three treatment groups(P<0.01). (3) The antihypertensive effects and improvement of aortic collagen deposition were better in the Tongyang and Tongluo groups than those in the Hanjingtang group(P<0.05, P<0.01). Conclusion Hanjingtang can significantly reduce the serum inflammatory factors of IL-1β, TNF-α and IL-6, improve endothelial function, reduce collagen deposition in blood vessels, improve aortic fibrosis, partially explaining the antihypertensive mechanism of Hanjingtang. The Tongyang ingredients can mainly reduce inflammatory factors, while Tongluo ingredients can regulate NO / ET-1 levels to play a synergistic role.

Key words: Hanjingtang, Hypertension with cold syndrome, Aortic fibrosis, Inflammation factors, Endothelial dysfunction

CLC Number: 

  • R285.5
[1] 赵连友. 高血压学[M]. 北京: 科学出版社, 2019: 50.
[2] Chamarthi B, Williams JS, Williams GH. A mechanism for salt-sensitive hypertension: abnormal dietary sodium-mediated vascular response to angiotensin-II [J]. J Hypertens, 2010, 28(5): 1020-1026.
[3] 王青梅, 郭宏, 田野. 寒冷暴露与高血压[J]. 中华高血压杂志, 2013, 21(1): 21-24.
[4] Takeda R, Stickford Abigail SL, Best Stuart A, et al. Effects of salt intake on sympathetic neural and pressor responses to cold pressor test in premenopausal women with a history of normal pregnancy [J]. Am J Physiol Regul Integr Comp Physiol, 2021, 320(3): R307-R316.
[5] Yao FR, Sun CW, Chang SKC. Lentil polyphenol extract prevents angiotensin II-induced hypertension, vascular remodelling and perivascular fibrosis [J]. Food Funct, 2012, 3(2): 127-133.
[6] Jayedi A, Rahimi K, Bautista LE, et al. Inflammation markers and risk of developing hypertension: a meta-analysis of cohort studies [J]. Heart, 2019, 105(9): 686-692.
[7] Angeli F, Reboldi G, Verdecchia P. The Link between inflammation and hypertension: unmasking mediators [J]. Am J Hypertens, 2021, 34(7): 683-685.
[8] 王春, 秦少博. 炎症与血管内皮损伤研究进展[J]. 心血管病学进展, 2015, 36(1): 89-92. WANG Chun, QIN Shaobo. Research progress of inflammation and vascular endothelial injury [J]. Advances in Cardiovascular Diseases, 2015, 36(1): 89-92.
[9] Lan HY. Diverse roles of TGF-β/Smads in renal fibrosis and inflammation [J]. Int J Biol Sci, 2011, 7(7): 1056-1067.
[10] Zhang S, Zhang Y, Ahsan MZ, et al. Atorvastatin attenuates cold-induced hypertension by preventing gut barrier injury [J]. J Cardiovasc Pharmacol, 2019, 74(2): 143-151.
[11] 韩晓清, 白仲艳, 杨阳. 李士懋运用寒痉汤治疗寒证痿痹经验[J]. 中医杂志, 2017, 58(21): 1815-1817. HAN Xiaoqing, BAI Zhongyan, YANG Yang. LI Shimaos experience in treating flaccidity-arthralgia of cold syndrome by using hanjing decoction [J]. Journal of Traditional Chinese Medicine, 2017, 58(21): 1815-1817.
[12] 马凯, 王四平, 康素刚, 等. 国医大师李士懋平脉辨证治疗冠心病思辨体系初探[J]. 河北中医, 2019, 41(8): 1125-1128.
[13] 林燕, 张明泉, 张琳琳, 等. 李士懋辨治寒凝证高血压病经验[J]. 中医杂志, 2017, 58(8): 644-646. LIN Yan, ZHANG Mingquan, ZHANG Linlin, et al. LI Shimaos experience in treating hypertension with cold stagnation syndrome [J]. Journal of Traditional Chinese Medicine, 2017, 58(8): 644-646.
[14] 郭娜. 汗法治疗阴寒凝滞型不稳定性心绞痛疗效及对患者血浆CRP、ET-1和NO的影响[J]. 陕西中医, 2020, 41(4): 465-467.
[15] 张洁晗, 张明泉. 寒冷与高盐饮食复合作用对大鼠血压的影响及中药的干预作用[J]. 时珍国医国药, 2018, 29(1): 250-251.
[16] 刘洋. 寒痉汤对寒凝型高血压大鼠CGRP、RLX及血管平滑肌细胞UCP2、RXFP2的影响[D]. 石家庄:河北中医学院, 2019.
[17] Loh YC, Chan SY, Tew WY, et al. New flavonoid-based compound synthesis strategy for antihypertensive drug development [J]. Life Sci, 2020, 249: 117512. doi: 10.1016/j.lfs.2020.117512.
[18] 司延如, 尹祾轩, 王国辉, 等. 天然黄酮类化合物对血压的调节作用机制研究进展[J]. 山东医药, 2020, 60(36): 108-111.
[19] Crespo I, García-Mediavilla MV, Gutiérrez B, et al. A comparison of the effects of kaempferol and quercetin on cytokine-induced pro-inflammatory status of cultured human endothelial cells [J]. Br J Nutr, 2008, 100(5): 968-976.
[20] 李红专, 刘行海, 牛彩琴. 附子水煎剂对家兔离体主动脉血管张力的影响[J]. 中国组织工程研究, 2015, 19(33): 5312-5317. LI Hongzhuan, LIU Xinghai, NIU Caiqin, et al. Tension effect of water decoction of Aconitum carmichaeli Debx on rabbit aortain vitro [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(33): 5312-5317.
[21] 杨钦, 毛以林. 基于网络药理学探讨附子-细辛对高血压病的作用机制[J]. 湖南中医杂志, 2021, 37(6): 149-155. YANG Qin, MAO Yilin. Mechanism of action of Radix Aconiti Lateralis Preparata-asarum in treatment of hypertension based on network pharmacology [J]. Hunan Journal of Traditional Chinese Medicine, 2021, 37(6): 149-155.
[22] 王晓明, 许良葵, 罗佳波. 麻黄-桂枝配伍对抗炎作用的影响及机制研究[J]. 时珍国医国药, 2020, 31(3): 528-531. WANG Xiaoming, XU Liangkui, LUO Jiabo. Effects of herba ephedrae-ramulus cinnamomi compatibility on anti-inflammation and mechanism study [J]. Lishizhen Medicine and Materia Medica Research, 2020, 31(3): 528-531.
[23] 张艺, 李廖英子, 韩爱庆, 等. 麻黄细辛附子汤拆方干预CD4+T细胞调控相关细胞因子分泌及STAT6 mRNA表达的研究[J]. 中国中医急症, 2021, 30(6): 950-954. ZHANG Yi, LI Liaoyingzi, HAN Aiqing, et al. Effect of mahuang xixin fuzi decoction on the intervention of CD4+T cell regulation related cytokine secretion and STAT6 mRNA expression[ J]. Journal of Emergency in Traditional Chinese Medicine, 2021, 30(6): 950-954.
[24] 李红蓉, 张肖, 常丽萍, 等. 通心络胶囊抗动脉粥样硬化研究进展[J]. 中成药, 2016, 38(2): 386-391.
[25] 吕明月, 吕巨伟, 张婧茜, 等. 四虫胶囊组方分析、药理作用及临床应用研究进展[J]. 广西医学, 2020, 42(21): 2861-2865. LYU Mingyue, LYU Juwei, ZHANG Jingxi, et al. Research progress in formulation analysis, pharmacological action and clinical application of Sichong Capsule [J]. Guangxi Medical Journal, 2020, 42(21): 2861-2865.
[26] 董彦文, 牧骑, 孙秀杰, 等. 寒冷刺激对急性心肌梗死内皮素、炎症因子的影响[J].心脑血管病防治, 2018, 18(2): 126-127.
[27] Sun ZJ, Wang XQ, Wood CE, et al. Genetic AT1A receptor deficiency attenuates cold-induced hypertension [J]. Am J Physiol Regul Integr Comp Physiol, 2005, 288(2): 433-439.
[28] Kanaji N, Sato T, Nelson A, et al. Inflammatory cytokines regulate endothelial cell survival and tissue repair functions via NF-κB signaling [J]. J Inflamm Res, 2011, 4: 127-138.
[29] Hoyles RK, Derrett-Smith EC, Khan K, et al. An essential role for resident fibroblasts in experimental lung fibrosis is defined by lineage-specific deletion of high-affinity type II transforming growth factor β receptor [J]. Am J Respir Crit Care Med, 2011, 183(2): 249-261.
[30] Rodriguez-Vita J, Ruiz-Ortega M, Rupérez M, et al. Endothelin-1, via ETA receptor and independently of transforming growth factor-beta, increases the connective tissue growth factor in vascular smooth muscle cells [J]. Circ Res, 2005, 97(2): 125-134.
[31] Rodríguez-Pascual F, Busnadiego O, González-Santamaría J. The profibrotic role of endothelin-1: is the door still open for the treatment of fibrotic diseases? [J]. Life Sci, 2014, 118(2): 156-164.
[32] Saura M, Zaragoza C, Herranz B, et al. Nitric oxide regulates transforming growth factor-beta signaling in endothelial cells [J]. Circ Res, 2005, 97(11): 1115-1123.
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