Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (7): 55-60.doi: 10.6040/j.issn.1671-7554.0.2019.475

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Anti-inflammation effect of baicalin on rat models of nonalcoholic steatohepatitis

AI Zhenglin1, HONG Shan1, HU Julong1, LI Ping1, ZHOU Yuling1, LIANG Xiuxia1, YAO Shukun2   

  1. 1. Department of Gastroenterology, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China;
    2. Department of Gastroenterology, China-Japan Friendship Hospital, Ministry of Health, Beijing 100029, China
  • Published:2022-09-27

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Abstract: Objective To investigate the efficacy of baicalin in the treatment of non-alcoholic steatohepatitis(NASH)in rat models and the anti-inflammatory effect. Methods After NASH rat models were induced, they were intragastrically given different doses of baicalin 15, 30, 60 mg/(kg·d). The body weight and liver weight were measured. The histopathology changes in hepatic steatosis were observed with hematoxylin-eosin(HE)staining. The serum alanine amiotransferase(ALT)and aspartate aminotransferase(AST)levels were assessed. The changes of hepatic reduced glutathione hormone(GSH), superoxide dismutase(SOD)and hepatic malondialdehyde(MDA)were detected with spectrophotometry. The levels of tumor necrosis factor α(TNFα), interleukin 6(IL-6)and interleukin 1β(IL-1β)in liver tissues were measured with enzyme linked immunosorbent assay(ELASA). Results After baicalin treatment, the body weight and liver weight of NASH rats were significantly reduced(F=1 609.79, P<0.001; F=155.17, P<0.001) 山 东 大 学 学 报 (医 学 版)57卷7期 -艾正琳,等.黄芩苷治疗非酒精性脂肪性肝炎大鼠抗炎的疗效 \=-in a dose-dependent manner. The steatosis of hepatic cells gradually decreased, and the infiltration of inflammatory cells was relieved. The serum ALT and AST reduced(F=177.16, P<0.001; F=2 998.11, P<0.001), the activity of GSH and SOD increased(F=398.47, P<0.001; F=256.07, P<0.001); the MDA;TNFα, IL-1β and IL-6 levels decreased(F=237.98, P<0.001;F=399.15, P<0.001; F=314.76, P<0.001; F=108.38, P<0.001), in a dose-dependent manner. Conclusion Baicalin can alleviate the degree of steatosis and inflammation in NASH rats. By increasing the activity of GSH and SOD enzymes, and reducing the levels of TNFα, IL-1β and IL-6, it exerts anti-inflammatory effects and alleviates liver inflammation.

Key words: Baicalin, Anti-inflammation, Nonalcoholic steatohepatitis, Tumor necrosis factor α, Interleukin

CLC Number: 

  • R575.1
[1] Seto WK, Yuen MF. Nonalcoholic fatty liver disease in Asia: emerging perspectives [J]. J Gastroenterol, 2017, 52(2): 164-174.
[2] Xu ZJ, Shi JP, Yu DR, et al. Evaluating the relationship between metabolic syndrome and liver biopsy-proven non-alcoholic steatohepatitis in China: a multicenter cross-sectional study design [J]. Adv Ther, 2016, 33(11): 2069-2081.
[3] Younossi ZM, Korenning AB, Adelatif D, et al. Global epidemiology of nonalcoholic fatty liver disease-Meta-analytic assessment of prevalence, incidence, and outcomes [J]. Hepatology, 2016, 64(1): 73-84.
[4] Sanyal AJ, Harrison SA, Ratziu V, et al. The natural history of advanced fibrosis due to nonalcoholic steatohepatitis: data from the simtuzumab trials [J]. Hepatology, 2019, 16. doi: 10.1002/hep.30664.
[5] Fan JG, Kim SU, Wong VW. New trends on obesity and NAFLD in Asia [J]. J Hepatol, 2017, 67(4): 862-873.
[6] Polyzos SA, Kountouras J, Mantaoros CS. Obesity and nonalcoholic fatty liver disease: From pathophysiology to therapeutics [J]. Metabolism, 2019, 92: 82-97. doi: 10.1016/j.metabol.2018.11.014.
[7] Yang J, Fernández-Galilea M, Martínez-Fernández L, et al. Oxidative stress and non-alcoholic fatty liver disease: effects of omega-3 fatty acid supplementation [J]. Nutrients, 2019,18,11(4): 872. doi: 10.3390/nu11040872.
[8] Chirumbolo S. Baicalin in flavocoxid may act against hepatitis B virus via a pro-inflammatory pathway [J]. Inflamm Res, 2018, 67(3): 203-205.
[9] Zhang J, Zhang H, Deng X, et al. Baicalin attenuates non-alcoholic steatohepatitis by suppressing key regulators of lipid metabolism, inflammation and fibrosis in mice [J]. Life Sci, 2018, 192: 46-54. doi: 10.1016/j.lfs.2017.11.027.
[10] He P, Wu Y, Shun J, et al. Baicalin ameliorates liver injury induced by chronic plus binge ethanol feeding by modulating oxidative stress and inflammation via CYP2E1 and NRF2 in mice [J]. Oxid Med Cell Longev, 2017, 2017:4820414. doi: 10.1155/2017/4820414.
[11] 艾正琳, 张伟硕, 姚树坤, 等. 黄芩苷对体外氧化应激模型中肝型脂肪酸结合蛋白表达的影响及意义[J]. 中华肝脏病杂志, 2011, 19(12): 53-57. AI Zhenglin, ZHANG Weishuo, YAO Shunkun, et al. Effect of baicalin on liver fatty acid binding protein in oxidative stress in vitro[J]. Chinese Jounal of Hepatology, 2011, 19(12): 53-57.
[12] 中华医学会肝病学分会脂肪肝和酒精性肝病学组. 非酒精性脂肪性肝病诊疗指南(2010年修订版)[J]. 中华肝脏病杂志, 2010, 18(3): 163-166. The Chinese National Workshop on Fatty Liver and Alcoholic Liver Disease for the Liver Disease Association. Guidelines for management of nonalcoholic fatty liver disease: an updated and revised edition [J]. Chinese Jounal of Hepatology, 2010, 18(3): 163-166.
[13] 中华医学会肝病学分会脂肪肝和酒精性肝病学组,中国医师协会脂肪性肝病专家委员会. 非酒精性脂肪性肝病诊疗指南(2018更新版)[J]. 中华肝脏病杂志, 2018, 26(3): 195-203.
[14] Lieber CS, Leo MA, Mak KM, et al. Model of nonalcoholic steatohepatitis [J]. Am J Clin Nutr, 2004, 79(3): 502-509.
[15] Sunaram V, Morgan TR. Will studies in nonalcoholic steatohepatitis help manage alcoholic steatohepatitis? [J]. Clin Liver Dis, 2019, 23(1): 157-165.
[16] Farzanegi P, Dana A, Ebrahimpoor Z, et al. Mechanisms of beneficial effects of exercise training on non-alcoholic fatty liver disease(NAFLD): Roles of oxidative stress and inflammation [J]. Eur J Sport Sci, 2019, 19(7): 994-1003.
[17] Chen K, Ma J, Jia X, et al. Advancing the understanding of NAFLD to hepatocellular carcinoma development: From experimental models to humans [J]. Biochim Biophys Acta Rev Cancer, 2019, 1871(1): 117-125.
[18] Liang Q, Chen H, Xu X, et al. miR-182-5p Attenuates high-fat -diet-induced nonalcoholic steatohepatitis in mice [J]. Ann Hepatol. 2019, 18(1): 116-125.
[19] Jia R, Du J, Cao L,et al. Antioxidative, inflammatory and immune responses in hydrogen peroxide-induced liver injury of tilapia(GIFT, Oreochromis niloticus)[J]. Fish Shellfish Immunol, 2018, 31(84): 894-905.
[20] Uchio R, Murosaki S, Ichikawa H. Hot water extract of turmeric(Curcuma longa)prevents non-alcoholic steatohepatitis in mice by inhibiting hepatic oxidative stress and inflammation [J]. J Nutr Sci, 2018, 27(7): 36. doi: 10.1017/jns.2018.27. eCollection 2018.
[21] Sutti S, Jindal A, Locatelli I, et al. Adaptive immune responses triggered by oxidative stress contribute to hepatic inflammation in NASH [J]. Hepatology, 2014, 59(3): 886-897.
[22] Bessone F, Razori MV, Roma MG. Molecular pathways of nonalcoholic fatty liver disease development and progression [J]. Cell Mol Life Sci, 2019, 76(1): 99-128.
[23] Chen Z, Yu R, Xiong Y, et al. A vicious circle between insulin resistance and inflammation in nonalcoholic fatty liver disease [J]. Lipids Health Dis, 2017, 16(1): 203. doi: 10.1186/s12944-017-0572-9.
[24] Henkel J, Coleman CD, Schraplau A, et al. Augmented liver inflammation in a microsomal prostaglandin E synthase 1(mPGES-1)-deficient diet-induced mouse NASH model [J]. Sci Rep, 2018, 8(1): 16127.
[25] Atay K, Canbakan B, Koroglu E, et al. Apoptosis and disease severity is associated with insulin resistance in non-alcoholic fatty liver disease [J]. Acta Gastroenterol Belg, 2017, 80(2): 271-277.
[26] Jorge ASB, Andrade JMO, Paraiso AF, et al. Body mass index and the visceral adipose tissue expression of IL-6 and TNF-alpha are associated with the morphological severity of non-alcoholic fatty liver disease in individuals with class III obesity [J]. Obes Res Clin Pract, 2018, 12(Suppl 2): 1-8.
[27] Nawaz R, Zahid S, Idrees M, et al. HCV-induced regulatory alterations of IL-1β, IL-6, TNF-α, and IFN-γ operative, leading liver en-route to non-alcoholic steatohepatitis [J]. Inflamm Res, 2017, 66(6): 477-486.
[28] Lambertucci F, Arboatti A, Sedlmier MG, et al. Disruption of tumor necrosis factor alpha receptor 1 signaling accelerates NAFLD progression in mice upon a high-fat diet [J]. J Nutr Biochem, 2018, 58: 17-27. doi: 10.1016/j.jnutbio.2018.04.013.
[29] Nelson JE, Handa P, Aouizerat B, et al. Increased parenchymal damage and steatohepatitis in Caucasian non-alcoholic fatty liver disease patients with common IL1B and IL6 polymorphisms [J]. Aliment Pharmacol Ther, 2016, 44(11-12): 1253-1264.
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