Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (7): 15-20.doi: 10.6040/j.issn.1671-7554.0.2017.583

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Effects of adenouvirus-mediated HBx on lymphocyte in vitro

BI Tao1, LU Qianqian2, SUN Chuandong3   

  1. 1. Department of Gastroenterology Surgery, Yantai Affiliated Hospital of Binzhou Medical University, Yantai 264100, Shandong, China;
    2. First Department of Oncology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai 264100, Shandong, China;
    3. Department of Hepatobiliary Surgery, The Affiliated Hospital of Qingdao University, Qingdao 266000, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To investigate the replication of adenovirus-mediated hepatitis B virus x gene(Ad-HBx)in lymphocytes and its effects on the secretion of interleukin-10(IL-10)and apoptosis of lymphocytes. Methods In vitro, lymphocytes collected from healthy human peripheral blood were transfected with Ad-HBx(Ad-HBx group)and Ad-EGFP(Ad-EGFP group). Non-treated lymphocytes served as the blank controls(Lymphocyte group). The expression of HBx in lymphocytes was detected with quantitative polymerase chain reaction(Q-PCR). The secretion of IL-10 was assessed with enzyme linked immunosorbent assay(ELISA). The apoptosis of lymphocytes was detected with flow cytometry. Results Q-PCR results showed that the expression of HBx was significantly higher in the Ad-HBx group than in the Ad-EGFP group and Lymphocyte group(P<0.05)48 h after transfection. ELISA results showed that the concentration of IL-10 was significantly lower in the Ad-HBx group than in the Ad-EGFP group and Lymphocyte group 12 h, 24 h, 36 h and 48 h after transfection(P<0.001). Flow cytometry results indicated that the apoptosis rate of Ad-HBx 山 东 大 学 学 报 (医 学 版)56卷7期 -毕涛,等. 腺病毒介导HBx对人淋巴细胞影响的体外实验 \=-group in the early stage was significantly higher than that of the Ad-HBx group and Lymphocyte group(P<0.05). Conclusion In vitro, HBx is highly expressed in healthy human peripheral blood lymphocytes. Ad-HBx inhibits the expression of IL-10 and promotes lymphocyte apoptosis in an early stage. HBx affects the regulation of human immune system possibly through gene expression, promotion of early apoptosis and inhibition of anti-inflammatory cytokines.

Key words: Adenouvirus, Hepatitis B virus x gene, Lymphocyte, Interleukin-10, Apoptosis

CLC Number: 

  • R575
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