Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (1): 38-44.doi: 10.6040/j.issn.1671-7554.0.2017.1038

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Effect and mechanism of magnesium isoglycyrrhizinate on the biological behavior of breast cancer cell lines

LI Yi, PENG Jing, ZHANG Lei, LU Jingsong   

  1. Department of Breast Disease, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200127, China
  • Published:2022-09-27

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Abstract: Objective To explore the effects and mechanism of magnesium isoglycyrrhizinate on the biological behavior of breast cancer. Methods Breast cell lines MDA-MB-231 and MCF-7 were treated with different concentrations(0, 50, 500 μmol/L)of magnesium isoglycyrrhizinate. Cell proliferation, migration and death were detected by CCK-8 assay, wound healing test, and LDH-cytotoxicity assay kit. Effect of magnesium isoglycyrrhizinate on the expressions of Bax, Bcl-2, Caspase-3, Caspase-9, HSP70 and HSP90 in breast cancer cells were examined by Western blotting. Results After treated with different concentrations of magnesium isoglycyrrhizinate, cell proliferation and migration abilities were inhibited in breast cancer cells, while autophagy and apoptosis increased. The inhibitory effects of platinum on cell migration were enhanced by the combination usage with magnesium isoglycyrrhizinate. Expression of HSP90 was reduced and Bax was promoted in MDA-MB-231 cell line. Expression of HSP70 was reduced in MCF-7 cell line. Conclusion Magnesium isoglycyrrhizinate inhibits the malignant phenotype of human breast cancer cell lines and has the synergic antitumor effect combined with platinum. The mechanism of magnesium isoglycyrrhizinate on MCF-7 and MDA-MB-231 cell lines are different.

Key words: Breast cancer, Magnesium isoglycyrrhizinate, Proliferation, Migration, Apoptosis, MDA-MB-231, MCF-7

CLC Number: 

  • R73-36+1
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