Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (8): 34-43.doi: 10.6040/j.issn.1671-7554.0.2021.1339

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Construction of a prognostic risk model of pyroptosis-related genes in breast cancer based on database

HE Shiqing1, LI Wanwan1, DONG Shuqing1, MOU Jingyi1, LIU Yuying1, WEI Siyu1, LIU Zhao2, ZHANG Jiaxin2   

  1. 1.Xuzhou Medical University, Xuzhou 221004, Jiangsu, China;
    2. Department of Thyroid and Breast Surgery, The Affiliated Hospital of Xuzhou Medical University, Xuzhou 221004, Jingsu, China
  • Published:2022-07-27

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Abstract: Objective To explore the prognostic value of pyroptosis-related differentially expressed genes(DEGs)in breast cancer and to construct a prognostic risk model. Methods Gene sequencing and clinical data of breast cancer were downloaded from The Cancer Genome Atlas(TCGA)and Gene Expression Ominibus(GEO)to screen for pyroptosis-related DEGs. A cluster analysis was performed on breast cancer patients. The model of TCGA cohort was established by the least absolute shrinkage and selection operator(LASSO)method, which was then evaluated with Kaplan-Meier survival curve, receiver operating characteristic curve(ROC), univariate and multivariate Cox regression independent prognostic factor analysis. The GEO cohort was used as the validation set. Enrichment of DGEs was analyzed with GO, KEGG, and ssGSEA. Results Pyroptosis-related DEGs were screened, cluster analysis showed that the overall survival(OS)of C2 group was prolonged, and the difference was statistically significant?(P=0.020). K-M survival analysis showed that OS was shortened in the high-risk group(P<0.001 in the TCGA cohort, P=0.018 in the GEO cohort). The area under the ROC curve(AUC)showed that the model had certain predictive ability. Univariate and multivariate Cox regression showed that age, M and N stage, and risk score were independent predictors of OS. GO, KEGG and ssGSEA analyses confirmed that pyroptosis-related DEGs were related to immune inflammatory factors and pathways. Conclusion This study constructed a prognostic risk model of breast cancer composed of 9 pyroptosis-related genes, which can provide reference for the risk and prognosis assessment of breast cancer patients.

Key words: Breast cancer, Pyroptosis, Immunity, Prognosis, Prediction model

CLC Number: 

  • R737.9
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