Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (9): 67-73.doi: 10.6040/j.issn.1671-7554.0.2022.0686

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Changes of intestinal B lymphocytes in 6 mice with non-alcoholic fatty liver disease

ZHANG Feng, WU Zhe, XU Jun, LIU Yulan   

  1. Department of Gastroenterology, Peking University Peoples Hospital, Beijing 100044, China
  • Published:2022-09-02

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Abstract: Objective To investigate the changes of intestinal B lymphocytes in mice with non-alcoholic fatty liver disease(NAFLD)induced by high fat diet. Methods A total of 12 male C57BL/6J mice were randomly divided into the experimental group and control group, with 6 in either group. The experimental group was fed with 45% high fat diet, while the control group with normal diet. After 16 weeks, the mice were killed to collect liver, small intestine mucosa, colon mucosa, mesenteric lymph nodes(MLN), Peyers patches(PP)and small intestine contents. Hepatic steatosis in experimental group was confirmed with hematoxylin and eosin(HE)staining. The distribution and expression of B cells in small intestine and colon mucosa were detected with immunohistochemical staining and real-time quantitative PCR, respectively. Proportion of B and T lymphocytes in the MLN and PP were analyzed with flow cytometry. The sIgA level in the small intestinal contents was detected with enzyme linked immunosorbent assay(ELISA). B lymphocytes were enriched with a magnetic-activated cell sorting method and cultured in vitro with or without the stimulation of lipopolysaccharide(LPS)or anti-CD40/IgM(BCR). The levels of IL-6, IL-10 and TNF-α in the cultural supernatants were monitored with cytometric bead arrays(CBA). Results B lymphocytes scattered throughout the lamina in both groups. The proportion of B lymphocytes was increased significantly in MLN and PP in experimental group than in control group(PMLN-B=0.025, PPP-B=0.004). The experimental group had higher level of sIgA in the small intestinal contents(P=0.042), which was in line with the higher proportion of IgA+ B lymphocytes in the PP(P=0.023). B lymphocytes in MLN in the experimental group secreted more IL-6 upon the stimulation of LPS or anti-CD40/IgM(BCR)(PLPS<0.001, PBCR=0.003)than in the control group. Conclusion Proportion of B lymphocytes in PP and MLN increased significantly in NAFLD mice. Small intestine secreted higher level of sIgA, and B lymphocytes in MLN produced more IL-6 upon stimulation in NAFLD mice.

Key words: Non-alcoholic fatty liver disease, B lymphocyte, Gut, Gut-liver axis, Inflammatory cytokine

CLC Number: 

  • R575
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