Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (9): 58-63.doi: 10.6040/j.issn.1671-7554.0.2020.137

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Correlation between interleukin-18 single nucleotide polymorphism and susceptibility of laryngeal squamous cell carcinoma: a report of 73 cases

MENG Zhaotun1, LI Qin2, SUN Hui3, WU Xinfang4, SUN Wenkai4, GAO Wei5   

  1. 1. Medical Department, Qingdao University, Qingdao 266071, Shandong, China;
    2. Department of Otorhinolaryngology, Linyi Peoples Hospital, Linyi 276000, Shandong, China;
    3. Weifang Clinical Medical College, Weifang Medical University, Weifang 261053, Shandong, China;
    4. School of Clinical Medicine, Binzhou Medical University, Binzhou 256603, Shandong, China;
    5. Graduate School, Shandong First Medical University, Taian 271016, Shandong, China
  • Online:2020-09-10 Published:2020-08-30

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Abstract: Objective To investigate the correlation between interleukin-18(IL-18)and promotors -137G/C and -607C/A gene polymorphisms and the susceptibility of laryngeal squamous cell carcinoma(LSCC)in a Chinese Han population in Shandong Province. Methods A total of 73 LSCC patients(case group)treated at Linyi Peoples Hospital during May 2017 and Oct. 2019 and 73 healthy controls were enrolled. The clinical information and peripheral venous blood samples(2 mL)were collected. The whole blood DNA was extracted, and gene sequencing and SNP genotyping 山 东 大 学 学 报 (医 学 版)58卷9期 -孟朝暾,等.白细胞介素18单核苷酸多态性与73例喉鳞状细胞癌易感性的关联性 \=-were conducted. The quantitative data were analyzed with Students-t test, and the qualitative data were analyzed with Pearson χ2 test or Fisher exact test. The control group was analyzed with chi-square goodness of fit test to verify whether it was in accordance with the Hardy-Weinberg equilibrium law. The correlation between two SNP loci and risk factors(age, smoking, drinking)with LSCC risk was determined with binary Logistic regression analysis. Results The case group had higher genotype frequency of GC at -137G/C(34.2% vs 19.2%, P=0.040), but there was no significant difference in the genotype frequency at -607C/A between the two groups(P>0.05). Smoking and drinking increased the risk of LSCC(OR=5.710; 3.116). After the risk factors of genotype, age, smoking and drinking history were adjusted, the OR of LSCC risk in smokers and -137G/C carriers were 4.967(95%CI=2.185-11.289, P<0.001)and 2.971(95%CI=1.243-7.101, P=0.014), respectively. Conclusion Our findings suggest that IL-18 promoter -137G/C polymorphism is correlated with an increased risk of LSCC.

Key words: Laryngeal squamous cell carcinoma, Single nucleotide polymorphism, Interleukins-18, Susceptibility, Risk factor

CLC Number: 

  • R767
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