Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (5): 80-86.doi: 10.6040/j.issn.1671-7554.0.2019.021

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Effects of interleukin-1β mediated ERK pathway on extracellular matrix of rat condylar chondrocytes

CHEN Hongyu1,2,QU Zhuli3, SUN Qi4, ZHAO Huaqiang1,5, MA Chuan1,5   

  1. 1. Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan 250012, Shandong, China;
    2. Department of Emergency, Stomatological Hospital of Shandong University, Jinan 250012, Shandong, China;
    3. Oral Department of Shandong Medical College, Jinan 250002, Shandong, China;
    4. Oral and Maxillofacial Surgery Department of Jinan Stomatological Hospital, Jinan 250001, Shandong, China;
    5. Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Shandong University, Jinan 250012, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To explore the effects of interleukin-1β mediated extracellular regulated protein kinases(ERK)pathway on extracellular matrix of rat condylar chondrocytes. Methods Condylar chondrocytes of Wistar rats were cultured and identified, which were then treated with different concentrations of IL-1β. The mRNA expressions of ERK, SRY-related high mobility group-box 9(Sox-9), type Ⅱ collagen(COL2), and the protein expressions of ERK, phosphorylated ERK(P-ERK), Sox-9 and COL2 were detected. The baseline IL-1β concentration which could induce cellular inflammation and activate ERK signaling pathway was determined. Based on the baseline IL-1β concentration, the 山 东 大 学 学 报 (医 学 版)57卷5期 -陈虹瑜,等.IL-1β介导的ERK通路对大鼠髁突软骨细胞外基质的作用 \=-condylar chondrocytes were divided into 3 groups: control group, IL-1β group and IL-1β+inhibitor group. The mRNA expression levels of ERK, Sox-9, COL2, and protein expression levels of ERK, P-ERK, Sox-9 and COL2 of all groups were detected. Results Cultured cells were identified as condylar chondrocytes by immunohistochemistry. The expression of P-ERK increased (P<0.05), while the expressions of Sox-9 and COL2 significantly decreased(all P<0.05)when treated with 10 ng/mL concentration of IL-1β. Compared with the IL-1β group, the IL-1β+inhibitor group had inhibited expression of P-ERK (P<0.05), but elevated expressions of Sox-9 and COL2(all P<0.05). Conclusion IL-1β is able to inhibit the expression levels of Sox-9 and COL2 by activating the ERK pathway of condylar chondrocytes.

Key words: Condylar chondrocytes, Interleukin-1β, Extracellular regulated protein kinases signaling pathway, SRY-related high mobility group-box 9, Type Ⅱ collagen

CLC Number: 

  • R782
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