Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (5): 74-79.doi: 10.6040/j.issn.1671-7554.0.2018.1332

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Anticancer effect of chrysophanol on colorectal cancer

WAN Xiuxia1, SUN Xiuping2, TONG Jingjie2   

  1. 1. Laboratory of Human Function, Basic Medical College, Qingdao University, Qingdao 266071, Shandong, China;
    2. Department of Medicine, School Hospital of Qingdao University, Qingdao 266071, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To explore the anticancer effect of chrysophanol on colorectal cancer. Methods The viability of colorectal cancer cells was detected with CCK-8. The apoptosis was determined with Tunel and Annexin V-FITC/PI staining. The protein expression was assessed with Western blotting. The tumor xenograft mice models were established and the body weight and tumor column were observed. Results The 2 μmol/L and 5 μmol/L chrysophanol increased the positive rate of Tunel from(1.3±0.3)% to(8.5±1.3)% and(18.6±2.9)% (n=3, F=66.7, P<0.01), respectively; increased the apoptosis rate of Annexin V-FITC/PI from(4.2±0.3)% to(9.6±1.3)% and(19.2±2.6)% (n=3, F=60.83, P<0.01), respectively; decreased the 48 h activity of HCT116 colon cancer cells to(77.2±6.5)% and(61.3±7.2)% (n=3, F=5.97, P<0.01), respectively. Caspase-9 and Caspase-3 specific inhibitors could reduce the apoptosis rate induced by chrysophanol from(19.2±2.6)% to(6.0±0.4)% and(7.2±0.6)% (n=3, F=56.37, P<0.01), respectively. Daily dosage of 10 mg/kg and 20 mg/kg chrysophanol could inhibit the volume of transplanted tumor in mice (n=6, F=9.48-13.79, P<0.01), and increase the apoptosis rate from(1.8±0.5)% to(9.7±1.6)% and(16.3±3.0)% (n=3, F=40.16, P<0.01), respectively. Conclusion Chrysophanol can be a potential candidate drug for the treatment of colorectal cancer.

Key words: Chrysophanol, Colorectal cancer, Apoptosis, Mitochondrial pathway, Mouse

CLC Number: 

  • R735.3
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