JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (5): 5-9.doi: 10.6040/j.issn.1671-7554.0.2015.088

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The inhibitory effect of baicalin on endothelial cell apoptosis induced by ox-LDL

YU Xin, LIU Xiaojing, LIU Xiangqun   

  1. Department of Cadre Care, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2015-01-25 Revised:2015-03-11 Online:2015-05-10 Published:2015-05-10

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Abstract: Objective To explore the possible mechanism of baicalin on the apoptosis of endothelial cells in the development of atherosclerosis (AS). Methods Endothelial cells were extracted from Wistar rats' thoracic aorta and divided into normal control group, baicalin treatment group, and ox-LDL treatment group. The control group was cultured with 10% FBS ECM, while the baicalin treatment group, and ox-LDL treatment group were cultured with different concentrations of baicalin and ox-LDL (10, 25, 50 μg/mL) respectively for 24 hours. The apoptosis of cells was determined with flow cytometry, and expressions of Bax, Bcl-2 and Caspase-3 mRNA were measured with RT-PCR. After that, cells were treated with baicalin plus ox-LDL(50 μg/mL) for 24 hours and the above indexes were measured. Results After baicalin treatment, Bcl-2 mRNA expression increased (P<0.01), while Caspase-3, Bax mRNA and Bax/Bcl-2 ratio decreased (all P<0.01). Baicalin significantly inhibited apoptosis of endothelial cells in a concentration-dependent manner (P<0.01). Compared with the control group and ox-LDL group of the same concentration, cells treated with baicalin had markedly elevated expressions of Bcl-2 mRNA, Bax and Caspase-3 and Bax/Bcl-2 ratio. Compared with cells treated with ox-LDL, cells treated with baicalin plus ox-LDL had significantly reduced apoptosis rate (P<0.01). Conclusion Baicalin can prevent cell damage caused by ox-LDL by upregulating expression of Bcl-2 and downregulating expressions of Caspase-3 and Bax.

Key words: Apoptosis, Baicalin, Caspase-3, Atherosclerosis

CLC Number: 

  • R541.4
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