JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (9): 8-12.doi: 10.6040/j.issn.1671-7554.0.2015.219

Previous Articles     Next Articles

Effects of factors secreted by mouse embryonic stem cells on prostate cancer cells in vitro

ZHAI Hongyun1, ZHANG Denglu1, WANG Guangjie1, KONG Feng2, CHENG Guanghui2, ZHAO Shengtian1   

  1. 1. Department of Urology, Jinan 250033, Shandong, China;
    2. Central Laboratory, Second Hospital of Shandong University, Jinan 250033, Shandong, China
  • Received:2015-02-28 Online:2015-09-10 Published:2015-09-10
  • Contact: 赵升田。E-mail:zhaoshengtian@sdu.edu.cn E-mail:zhaoshengtian@sdu.edu.cn

PDF (PC)

303

Abstract: Objective To study the effects of factors secreted by mouse embryonic stem cells (ESC) on the biological characteristics of prostate cancer cells RM-1 and to explore the mechanism. Methods A non-contact co-culture system was established with Transwell chambers in vitro. The chambers connecting ESC combined with mouse embryonic fibroblasts (MEF) and RM-1 cells served as experimental group, while the chambers connecting MEF and RM-1 cells were negative controls. After cells were co-cultured for 72 h, the proliferation of RM-1 cells in the two groups were measured. Differences of RM-1 cells in cell morphology, cell cycle, apoptotic level, migration and invasion ability between the two groups were observed. Results The RM-1 cells in experimental group were loosely arranged with less cell division and more cell death, and some cells showed signs of apoptosis. The RM-1 cells in experimental group proliferated more slowly (P<0.01), with cell cycle arrest between phase G1 and S in addition with increased apoptosis, especially late stage apoptosis (both P<0.01). The RM-1 cells in experimental group showed weaker migration and invasion ability (both P<0.05). Conclusion The factors secreted by ESC exhibited anti-tumor effects. They inhibited RM-1 cell proliferation by arresting cell cycle, increasing cell apoptosis, and weakening cell migration and invasion ability.

Key words: Secreted factors, Prostate cancer cell RM-1, Cellular environment, Embryonic stem cell, Mouse, Neoplasia

CLC Number: 

  • R737.25
[1] Abbott DE, Postovit LM, Seftor EA, et al. Exploiting the convergence of embryonic and tumorigenic signaling pathways to develop new therapeutic targets[J]. Stem Cell Rev, 2007, 3(1): 68-78.
[2] Costa FF, Seftor EA, Bischof JM, et al. Epigenetically reprogramming metastatic tumor cells with an embryonic microenvironment[J]. Epigenomics, 2009, 1(2): 387-398.
[3] Bissell MJ, Hines WC. Why don't we get more cancer? A proposed role of the microenvironment in restraining cancer progression[J]. Nat Med, 2011, 17(3): 320-329.
[4] Joel M, Sandberg CJ, Boulland JL, et al. Inhibition of tumor formation and redirected differentiation of glioblastoma cells in a xenotypic embryonic environment[J]. Dev Dyn, 2013, 242(9): 1078-1093.
[5] Postovit LM, Margaryan NV, Seftor EA, et al. Human embryonic stem cell microenvironment suppresses the tumorigenic phenotype of aggressive cancer cells[J]. Proc Natl Acad Sci U S A, 2008, 105(11): 4329-4334.
[6] Palumbo A Jr, Ferreira LB, Reis de Souza PA, et al. Extracellular matrix secreted by reactive stroma is a main inducer of pro-tumorigenic features on LNCaP prostate cancer cells[J]. Cancer Lett, 2012, 321(1): 55-64.
[7] Tandon N, Marolt D, Cimetta E, et al. Bioreactor engineering of stem cell environments[J]. Biotechnol Adv, 2013, 31(7): 1020-1031.
[8] Cetnar JP, Beer TM. Personalizing prostate cancer therapy: the way forward[J]. Drug Discov Today, 2014, 19(9): 1483-1487.
[9] Jozefczuk J, Drews K, Adjaye J. Preparation of mouse embryonic fibroblast cells suitable for culturing human embryonic and induced pluripotent stem cells[J]. J Vis Exp, 2012, 64, pii: 3854. doi: 10.3791/3854.
[10] Catalano V, Turdo A, Di Franco S, et al. Tumor and its microenvironment: a synergistic interplay[J]. Semin Cancer Biol, 2013, 23(6 Pt B): 522-532.
[11] Liu W, Vivian CJ, Brinker AE, et al. Microenvironmental influences on metastasis suppressor expression and function during a metastatic cell's journey[J]. Cancer Microenviron, 2014, 7(3): 117-131.
[12] Zhang X, Nie D, Chakrabarty S. Growth factors in tumor microenvironment[J]. Front Biosci, 2010, 15: 151-165.
[13] Hanna E, Quick J, Libutti SK. The tumor microenvironment: a novel target for cancer therapy[J]. Oral Dis, 2009, 15(1): 8-17.
[14] 詹秀琴, 姜泽群. 干细胞共培养技术在医学研究中的应用[J]. 中国细胞生物学学报, 2014, 36(8): 1178-1185. ZHAN Xiuqin, JIANG Zequn. Cell co-culture technique in the medical research of stem cells[J]. Chinese Journal of Cell Biology, 2014, 36(8): 1178-1185.
[15] Lu X, Park SH, Thompson TC, et al. Ras-induced hyperplasia occurs with mutation of p53, but activated ras and myc together can induce carcinoma without p53 mutation[J]. Cell, 1992, 70(1): 153-161.
[16] 童锦禄, 冉志华. G1/S检测点调控机制研究进展[J]. 世界华人消化杂志, 2005, 13(16): 2036-2039.
[17] Ouyang L, Shi Z, Zhao S, et al. Programmed cell death pathways in cancer: a review of apoptosis, autophagy and programmed necrosis[J]. Cell Prolif, 2012, 45(6): 487-498.
[18] Jäger R, Zwacka RM. The enigmatic roles of caspases in tumor development[J]. Cancers (Basel), 2010, 2(4): 1952-1979.
[19] Yilmaz M, Christofori G. Mechanisms of motility in metastasizing cells[J]. Mol Cancer Res, 2010, 8(5): 629-642. [20] Stetler-Stevenson WG, Gavil NV. Normalization of the tumor microenvironment: evidence for tissue inhibitor of metalloproteinase-2 as a cancer therapeutic[J]. Connect Tissue Res, 2014, 55(1): 13-19.
[21] Hendrix MJ, Seftor EA, Seftor R, et al. Reprogramming metastatic tumour cells with embryonic microenvironments[J]. Nat Rev Cancer, 2007, 7(4): 246-255.
[22] Giuffrida D, Rogers IM, Nagy A, et al. Human embryonic stem cells secrete soluble factors that inhibit cancer cell growth[J]. Cell Prolif, 2009, 42(6): 788-798.
[23] Buzhor E, Leshansky L, Blumenthal J, et al. Cell-based therapy approaches: the hope for incurable diseases[J]. Regen Med, 2014, 9(5): 649-672.
[24] Schulman IH, Suncion V, Karantalis V, et al. Clinical research skills development program in cell-based regenerative medicine[J]. Stem Cells Transl Med, 2015, 4(2): 118-122.
[1] YANG Xiao-Qian, JI Jing, LIU Na, GUO Dong-Mei, CUI Xi. Effect of FeCl3 and FeEDTA on inhibiting psoriasis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 114-117.
[2] SUO Dongyang, SHEN Fei, GUO Hao, LIU Lichang, YANG Huimin, YANG Xiangdong. Expression and mechanism of Tim-3 in animal model of drug-induced acute kidney injury [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 1-6.
[3] LONG Tingting, XIE Ming, ZHOU Lu, ZHU Junde. Effect of Noggin protein on learning and memory abilities and the dentate gyrus structure after cerebral ischemia reperfusion injury in mice [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 15-23.
[4] ZHU Lin, LI Weiwei, LIU Zhikai. Human stromal vascular fraction combined with adipose stem cells promotes the healing of radiation skin damage in nude mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(9): 66-72.
[5] LIU Jingkang, YANG Jianyong, MENG Lihua, JIANG Jie. The value of serum miR-17-92 cluster in early diagnosis of HPV-positive cervical cancer [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(5): 86-90.
[6] LI Hongzhi, SHEN Yongchao, LIU Jieting, ZHAO Xiaojin, CHU Yanhui, YUAN Xiaohuan. An 11β-HSD1 inhibitor improves insulin sensitivity in db/db mice [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(10): 59-64.
[7] WANG Yafen, YANG Yongxia, LIU Ya, MA Demei, ZHU Lin. Use of Cervista HPV HR A9 group virus to identify patients with negative cytological but positive high-risk HPV [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(8): 69-71.
[8] SHI Rui, SUN Pei, WANG Lulu, DING Lin, XIA Jin, WANG Yan, PANG Shuguang. Vitamin D and mecobalamin combined with mouse nerve growth factor for diabetic peripheral neuropathy: a clinical observation [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(4): 64-67.
[9] PENG Hui, HE Junmei, SU Xuelian. Expression of serum miR-375 and its clinical significance in the diagnosis and postoperative monitoring of cervical cancer [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(3): 68-71.
[10] JIA Junwei, ZHOU Xueying, LI Qiuhong, YU Kai, ZHOU Guoyu, ZHOU Shengnian. Expression of Annexin A7 in brain tissue of pilocarpine-induced epilepsy mouse model [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(10): 6-10.
[11] LI Dumin, WANG Qian, LI Chuanfu, WANG Qing. Two cases of multpile endoncrine neoplasia type 1 with Whipple syndrome [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(1): 62-66.
[12] TIAN Xinli, JIAO Jun, ZHANG Teng, MA Daoxin, CUI Baoxia. Expression and significance of Th22 and Th17 cells in the peripheral blood of cervical cancer [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(7): 43-47.
[13] XIAO Fang, JIANG Weidong, WANG Xiaohong, QIN Aiqiong, HAN Min, CHEN Li. Extraction and identification of mouse islet microvascular endothelial cells (MS-1)-derived exosomes [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(1): 6-9.
[14] ZHANG Zhongxia, MA Xiaowei, WANG Yanyong, LI Xiaoli, WANG Mingwei. Impact of aging on the nigro-strital oxidative stress in a mice model of Parkinson's disease [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(9): 26-29.
[15] ZHANG Lu, YAN Lei, ZHANG Hui, ZHANG Xiaohui, LI Mingjiang, ZHAO Xingbo. Expression and clinical significance of sperm-associated antigen 9 in patients with cervical cancer and cervical intraepithelial neoplasia [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(6): 67-71.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd