JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (10): 6-10.doi: 10.6040/j.issn.1671-7554.0.2016.247

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Expression of Annexin A7 in brain tissue of pilocarpine-induced epilepsy mouse model

JIA Junwei1, ZHOU Xueying2, LI Qiuhong1, YU Kai1, ZHOU Guoyu3, ZHOU Shengnian1   

  1. 1. Department of Neurology, Qilu Hospital of Shandong University &
    Brain Science Research Institute, Shandong University;
    2. Department of Rehabilitation Medicine, Qilu Hospital of Shandong University;
    3. Department of Cadre Health Care, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2016-03-09 Online:2016-10-10 Published:2016-10-10

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Abstract: Objective To explore the expression of Annexin A7 in brain tissue of pilocarpine-induced epilepsy mouse model. Methods Thirty healthy adult male C57BL/6 mice were randomly divided into experimental group(n=20)and control group(n=10). Epilepsy mouse model was established in experimental group and behavioural characteristics of mice were observed in two groups. Expression of Annexin A7 was detected using Western blotting and immunofluorescence labeling techniques. Results Eighteen mice suffered from status epilepticus and only eight survived in the experimental group. Of which, six mice encountered spontaneous seizures in experimental group. Status epilepticus and spontaneous seizures did not occur in control group. Expression of Annexin A7 in the hippocampus and temporal cortex of experimental group were markedly higher than that of control group(P<0.05). In the brain tissue of epilepsy mouse model, Annexin A7 was expressed in the neurons rather than astrocytes. Conclusion Annexin A7 may be involved in the occurrence and development of epilepsy.

Key words: Epilepsy, Mouse, Brain tissue, Pilocarpine, Annexin A7

CLC Number: 

  • R742.1
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