JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2014, Vol. 52 ›› Issue (2): 29-32.doi: 10.6040/j.issn.1671-7554.0.2013.543

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The effect of Metformin on the migration and invasion of renal cell carcinoma cells in vitro and its mechanism

LAI Cheng-jun1,2, QU Hong-hua3, GUO Xiao-yu4, XING Zhao-quan2, ZHOU Zun-lin2, XU Zhong-hua2, LIU Zhao-xu2,5   

  1. 1. Department of Urology, People′s Hospital of Qingyun, Qingyun 253700, Shandong, China;
    2. Department of Urology, Qilu Hospital of Shandong University, Jinan 250012, China;
    3. Department of Gynaecology and Obstetrics, Qilu Hospital of Shandong University, Jinan 250012, China;
    4. Provincial Hospital Affiliated to Shandong University, Jinan 250012, China;
    5. School of Nursing, Shandong University, Jinan 250012, China
  • Received:2013-08-05 Online:2014-02-10 Published:2014-02-10

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Abstract:

Objective   To explore the effect of Metformin on the migration and invasion of renal cell carcinoma (RCC) cells in vitro and its mechanism. Methods  The changes in the metastasis of 786-O cells after Metformin treatment were observed with Transwell assays. The expressions of phosphorylation AMP-activated protein kinase (p-AMPK), phosphoryltion mammalian target of rapamycin (p-mTOR), matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) were detected with immunoblotting. Results  Metformin restrained the migration and invasion of 786-O cells, activated AMPK, thus suppressing mTOR. Besides, metformin inhibited the expressions of MMP-2 and MMP-9. Conclusion  Metformin can suppress metastasis of renal cancer cells in vitro, suggesting its potential as a therapeutic agent for RCC.

Key words: Renal cell carcinoma, Metastasis, Metformin, AMP-activated protein kinase

CLC Number: 

  • R737.25
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