Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (3): 54-59.doi: 10.6040/j.issn.1671-7554.0.2017.1202

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Berberines effects on the proliferation, apoptosis and DNA damage and repair of human renal cell carcinoma cells

LI Xiaofeng1,2, DU Xiaoyi1, LIU Hainan1, LIU Cheng3, FAN Yidong1   

  1. 1. Department of Urology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    2. Central Laboratary, the Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    3. Department of Urology, Peking University Third Hospital, Beijing 100191, China
  • Received:2017-12-06 Published:2022-09-27

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Abstract: Objective To determine the effects of berberine(BBR)on the growth and DNA damage of human renal cell carcinoma(RCC)cells. Methods The effects of BBR on the proliferation of A498 and 786-O RCC cells at concentrations of 0-240 μmol/L were tested with CCK-8 testing kit. Based on the results of CCK-8 test, RCC cells were divided into control group(0 μmol/L), 30 μmol/L group and 60 μmol/L group. The cell apoptosis after BBR treatment was evaluated with Annexin V-FITC/PI Apoptosis Detection Kit. The expressions of proteins involved in the apoptotic pathway and DNA damage repair pathways, including pro-caspased3, cleaved-caspased3, γH2A.X and KU70, were assessed with Western blotting. Results The proliferation of RCC cells was significantly suppressed by BBR in a time- and dose-dependent manner(A498: P<0.001; 786-O: P=0.002). Cell apoptosis was obviously increased after BBR 山 东 大 学 学 报 (医 学 版)56卷3期 -李孝峰,等. 小檗碱对人肾细胞癌细胞增殖、凋亡、DNA断裂及损伤修复的影响 \=-treatment at an optimal concentration and time point. In comparison with the control group, the 30 and 60 μmol/L groups showed increased expressions of cleaved-caspase3(A498: P=0.018, P<0.001; 786-O: P=0.038, P<0.001), and γH2A.X(A498: P<0.001, P<0.001; 786-O: P<0.001, P<0.001), but decreased expression of KU70(A498: P=0.002, P<0.001; 786-O: P<0.001, P<0.001). Compared with the 30 μmol/L group, the 60 μmol/L group showed increased expressions of cleaved-caspase3(A498: P=0.020; 786-O: P=0.010), and γH2A.X(A498: P=0.002; 786-O: P<0.001), but decreased expression of KU70(A498: P<0.001; 786-O: P=0.005). Conclusion BBR can effectively suppress RCC cell proliferation, induce RCC cell apoptosis and DNA damage, and inhibit DNA damage and repair.

Key words: Renal cell carcinoma, Berberine, Cell apoptosis, Cell proliferation, DNA damage and repair

CLC Number: 

  • R737.11
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