Journal of Shandong University (Health Sciences) ›› 2023, Vol. 61 ›› Issue (10): 9-16.doi: 10.6040/j.issn.1671-7554.0.2023.0050

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Effects of Luling Anshen Granule on the inflammatory response and cell apoptosis in rats with chronic renal failure

YANG Yuanfeng1, XIONG Gaocai2, LI Yuchuan1, LUO Yuling1, ZHANG Jingjie3   

  1. 1. Department of Pharmacy, The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang 550003, Guizhou, China;
    2. Department of Respiratory and Critical Care, The Third Peoples Hospital of Guizhou Province, Guiyang 550000, Guizhou, China;
    3. School of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, Guizhou, China
  • Published:2023-11-08

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Abstract: Objective To investigate the effects of Luling Anshen Granule on adenine-induced chronic renal failure(CRF)rats and its potential mechanism. Methods Male SD rats were divided into 6 groups: control group, model group, positive drug group(Niaoduqing Granule, 2.25 g/kg), low-, medium- and high-dose Luling Anshen Granule groups(2.5, 5, 10 g/kg), with 10 rats in each group. Except the control group, the other groups were given 2.5% adenine suspension by gavage to establish CRF models, and corresponding drugs were given by gavage once a day for a consecutive of 28 days after successful modeling. The general condition of rats was observed and recorded. Blood was sampled from the abdominal aorta to measure the levels of blood urea nitrogen(BUN)and serum creatinine(Scr), and the level of 24-hour urinary protein was also measured. The levels of interleukin-1β(IL-1β), interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in serum were determined with ELISA. Histopathological changes of kidney tissues were observed with HE staining. The level of basic fibroblast growth factor(bFGF)in renal tissues was detected with immunohistochemistry. The mRNA expressions of Bax, Bcl-2 and Caspase-3, and the protein expressions of Bax, Bcl-2 and Cleaved-Caspase-3 in renal tissues were detected with qRT-PCR or Western blotting. Results Compared with the control group, the model group had more serious renal injury, and significantly increased levels of 24-hour urinary protein, BUN, Scr, IL-1β, IL-6, TNF-α, mRNA expressions of Bax/Bcl-2 and Caspase-3, protein expressions of Bax/Bcl-2 and Cleaved-Caspase-3, and bFGF(P<0.05). Compared with the model group, the Luling Anshen Granule intervention groups and positive drug group had significantly alleviated renal injury, and significantly decreased levels of the above indexes in a dose-dependent manner(P<0.05). Conclusion Luling Anshen Granule can inhibit the inflammatory response, cell apoptosis and fibrosis in kidney tissues of CRF rats and improve the renal function, thus having a protective effect on the kidney.

Key words: Chronic renal failure, Luling Anshen Granule, Inflammatory response, Cell apoptosis, Renal fibrosis

CLC Number: 

  • R285.5
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