Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (10): 79-85.doi: 10.6040/j.issn.1671-7554.0.2017.985

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Effects of TAZ knockdown on the proliferation and apoptosis of TSCC cell line CAL27 and the molecular mechanism

CHEN Xiyan1,2, WANG Qi1,2, GU Weiting3, WEN Yong1,2   

  1. 1. Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan 250012, Shandong, China;
    2. School of Stomatology, Shandong University, Jinan 250012, Shandong, China;
    3. Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2017-10-17 Published:2022-09-27

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Abstract: Objective To explore the effects of TAZ knockdown on the proliferation and apoptosis of tongue squamous cell carcinoma(TSCC)cell line CAL27, and the possible molecular mechanism. Methods CAL27 cells were divided into the interference group and control group. The former was transfected with targeting TAZ shRNA, and the latter with non-specific targeting sequence. The interference efficiency was detected with real-time fluorescent quantitative PCR(RT-PCR)and Western blotting. The changes of proliferation were detected with CCK-8 assay and EdU staining assay. The cell cycle distribution and cell apoptosis were tested with flowcytometry. The expressions of proteins related to cell cycle and apoptosis were determined with Western blotting. Results The results of RT-PCR and Western blotting showed 山 东 大 学 学 报 (医 学 版)56卷10期 -陈希彦,等.干扰TAZ基因对舌鳞癌细胞CAL27增殖凋亡的影响及其机制 \=-that the mRNA and protein expressions of the interference group decreased sufficiently. The CCK-8 and EdU staining assay indicated that knockdown of TAZ led to the inhibition of proliferation activity. Flowcytometry results showed that cell cycle arrested in G0/G1 phase and cell apoptosis increased after TAZ knockdown. Meanwhile, knockdown of TAZ decreased the expressions of CYCLIN D1, CDK4, and CDK6. By contrast, the expressions of proapoptotic proteins like Bax and cleaved Caspase9 increased, while the expression of anti-apoptotic protein like Bcl-2 decreased. Conclusion Knockdown of TAZ can affect the proliferation and apoptosis of TSCC cell line CAL27 by modulating the expression of proteins related to cell cycle and apoptosis.

Key words: TAZ gene, Tongue squamous cell carcinoma CAL27 cell line, Cell proliferation, Cell apoptosis

CLC Number: 

  • R739.8
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