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山东大学学报 (医学版) ›› 2021, Vol. 59 ›› Issue (12): 33-41.doi: 10.6040/j.issn.1671-7554.0.2021.0890

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集约化动物养殖区饲料-粪便-饮用水中β受体激动剂的残留特征及其健康风险

夏慧禹1,刘仲2,刘树民3,李学文1   

  1. 1. 山东大学齐鲁医学院, 山东 济南 250012;2. 济南市疾病预防控制中心理化检验所, 山东 济南 250021;3. 济南市长清区张夏镇畜牧兽医站, 山东 济南 250308
  • 发布日期:2021-12-29
  • 通讯作者: 李学文. E-mail:lxw@sdu.edu.cn

Residue characteristics and health risk of beta-agonists in fodder-faeces-drinking water in intensive animal breeding area

XIA Huiyu1, LIU Zhong2, LIU Shumin3, LI Xuewen1   

  1. 1.Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, China;
    2. Physical and Chemical Laboratory, Jinan Center for Disease Control and Prevention, Jinan 250021, Shandong, China;
    3. Animal Husbandry and Veterinary Station, Zhangxia Town, Changqing District, Jinan 250308, Shandong, China
  • Published:2021-12-29

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摘要： 目的 以中国东部某养殖区为例,探讨β受体激动剂的饲料添加情况和粪便残留特征,评估饮用水中β受体激动剂的人体健康风险。 方法 利用高效液相色谱-质谱联用(HPLC-MS/MS)检测不同养殖场饲料(n=70)、粪便(n=75)和饮用水(n=59)中12种β受体激动剂的含量,利用Kruskal-Wallis检验分析其在不同畜种粪便间的差异性,通过每日允许摄入量(ADI)评估其健康风险。 结果 该地区各类样品中均存在β受体激动剂残留。饲料中12种β受体激动剂的检出率均高于50%,其中溴布特罗检出率高达100%,非诺特罗浓度最高(102.94 μg/kg)。粪便中β受体激动剂的检出率高于80%,其中溴布特罗和溴代克伦特罗的检出率达到100%,浓度最高为非诺特罗(395.84 μg/kg)。不同畜禽粪便的β受体激动剂存在浓度差异,除特布他林外,其他11种物质均以牛粪中残留浓度最高(P<0.05)。饮用水中亦检出所有12种β受体激动剂,且检出率大部分在80%以上,其中溴布特罗检出率达到100%,非诺特罗浓度最高(30.09 ng/L)。通过单一饮用水暴露,克伦特罗和莱克多巴胺非致癌健康风险较低。 结论 该养殖区饲料中存在添加β受体激动剂的情况并导致较高的粪便残留,已通过环境扩散引起饮用水的污染,需引起相关部门的高度重视。

关键词: 畜禽, 集约化养殖, β受体激动剂, 饮用水, 健康风险

Abstract: Objective To investigate the residual characteristics of beta-agonists in fodder and faeces in an animal breeding area in eastern China, and to evaluate the human health risk of them in drinking water. Methods High performance liquid chromatography-mass spectrometry(HPLC-MS)was used to detect 12 beta-agonists in fodder(n=70), faeces(n=75)and drinking water(n=59)samples of different farms. Differences among faeces of different animal species were analyzed by Kruskal-Wallis test, and the health risks were evaluated by allowable daily intake(ADI). Results Beta-agonists were detected in all kinds of samples. The detection rate of 12 beta-agonists in fodder was higher than 50%. Detection rate of brombuterol was as high as 100%, and the concentration of fenoterol was the highest(102.94 μg/kg). Detection rate of beta-agonists in faeces was higher than 80%, and the detection rates of brombuterol and bromocreentrol were 100%; fenoterol was predominated(395.84 μg/kg)in faeces samples. The concentrations of beta-agonists in faeces of different livestock and poultry were statistical significant. Except terbutaline, the residual levels of other 11 substances in faeces were the highest in cattle farms(P<0.05). All beta-agonists were also detected in drinking water, and the detection rate of them was mostly more than 80%, of which brombuterol reached 100%, and the median concentration of fenoterol was the highest(30.09 ng/L). Through drinking water exposure, the non-carcinogenic health risk to human body of clenbuterol and ractopamine was relatively low. Conclusion A variety of beta-agonists were detected in the animal fodder and animal faeces in this study area. Beta-agonists in animal faeces can cause drinking water pollution through environmental diffusion. Thus, beta-agonists administrated in fodder needs to be paid great attention by local government.

Key words: Livestock and poultry, Intensive breeding, Beta-agonists, Drinking water, Health risk

中图分类号: 

  • R127
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