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山东大学学报 (医学版) ›› 2021, Vol. 59 ›› Issue (5): 40-45.doi: 10.6040/j.issn.1671-7554.0.2021.0308

• 医学病毒的基础与临床研究进展专题 • 上一篇    下一篇

新发突发病毒性传染病防控与生物安全

温红玲*(),徐小莹   

  1. 山东大学齐鲁医学院公共卫生学院微生物检验学系,山东省“十三五”高等学校感染性疾病防控重点实验室,山东 济南 250012
  • 收稿日期:2021-03-22 出版日期:2021-05-10 发布日期:2021-06-01
  • 通讯作者: 温红玲 E-mail:wenhongling@sdu.edu.cn
  • 作者简介:温红玲,医学博士,山东大学公共卫生学院教授,博士研究生导师。主要研究方向为分子病毒学与传染病流行病学。作为课题负责人主持科技部国家重点研发计划课题、国家自然科学基金项目、教育部博士点基金、山东省自然科学基金、山东省重点研发计划等10项,主持教研项目6项。获山东大学教学成果二等奖,山东大学青年教师教学比赛三等奖,两次获评山东大学课堂质量优秀教师。指导学生参加大学生公共卫生技能大赛,获全国团体二等奖和2项单项奖、山东省团体一等奖和多项单项奖。在LANCET INFECTIOUS DISEASES、Emerging Infectious Diseases、PLoS Neglected Tropical Diseases等国内外权威期刊发表学术论文100余篇,获国家发明专利1项。兼任中华医学会医学病毒学分会委员、中华预防医学会食品卫生分会青年委员、中国微生物学会微生物生物安全专业委员会青年委员、山东省医学会医学病毒学分会副主任委员、山东预防医学会理事、山东省微生物学会理事、山东省生物医药技术学会理事、山东预防医学会公共卫生教育分会委员和秘书、山东预防医学会卫生检验分会青年委员、山东省预防医学会艾滋病预防控制分会检测专业委员会委员、《中华实验和临床病毒学杂志》通讯编委、《中国病毒病杂志》青年编委等
  • 基金资助:
    山东省自然科学基金(ZR2018MH035);山东省重点研发计划(2019GSF107067)

Prevention and control of emerging viral infectious diseases and biosafety

Hongling WEN*(),Xiaoying XU   

  1. Department of Microbiological Laboratory Technology, School of Public Health, Cheeloo College of Medicine, Shandong University, Key Laboratory of Infectious Disease Control and Prevention in Universities of Shandong, Jinan 250012, Shandong, China
  • Received:2021-03-22 Online:2021-05-10 Published:2021-06-01
  • Contact: Hongling WEN E-mail:wenhongling@sdu.edu.cn

摘要:

近年来,新发突发病毒性传染病频发,尤其是动物源性病毒的跨种传播,给传染病防控和生物安全(如实验室生物安全、国境口岸生物安全、生物技术两用性等)带来了巨大挑战。目前,国际生物安全形势非常严峻,具有影响国际化、危害极端化、发展复杂化等特点,生物安全问题已成为危害国家安全的重大问题,各界对于病毒学和生物威胁的关注也达到一个前所未有的高度。本文通过对新发突发病毒性传染病的发生、防控及基础研究中的生物安全现状进行综述,总结了当前我国生物安全防御领域亟待解决的诸多问题,并对未来的生物安全领域发展方向进行展望。

关键词: 新发突发病毒性传染病, 生物技术两用性, 生物安全

Abstract:

In recent years, the frequent occurrences of emerging infectious diseases, especially the interspecies transmission of animal-borne viruses, have brought immense challenges to the prevention and control of infectious diseases and biosafety, such as laboratory biosafety, frontier port biosafety and dual-use biotechnology. Currently the international biosafety situation is serious, characterized with internationalization, extreme harm and complicated development. Biosafety has become a major issue endangering national security, which has aroused great concern about virology and biological threats. In this paper, the occurrence, prevention and control of emerging viral infectious diseases and the status quo of biosafety in basic research were reviewed, various problems were summarized, and the future development of biosafety was prospected.

Key words: Emerging viral infectious diseases, Dual-use biotechnology, Biosafety

中图分类号: 

  • R373
1 Liu WJ , Wu Y , Bi Y , et al. Emerging HxNy influenza a viruses[J]. Cold Spring Harb Perspect Med, 2020, 14, a038406.
doi: 10.1101/cshperspect.a038406
2 Petersen LR , Jamieson DJ , Powers AM , et al. Zika virus[J]. N Engl J Med, 2016, 374 (16): 1552- 1563.
doi: 10.1056/NEJMra1602113
3 Huff HV , Singh A . Asymptomatic transmission during the coronavirus disease 2019 pandemic and implications for public health strategies[J]. Clin Infect Dis, 2020, 71 (10): 2752- 2756.
doi: 10.1093/cid/ciaa654
4 Getz LJ , Dellaire G . Angels and devils: dilemmas in dual-use biotechnology[J]. Trends Biotechnol, 2018, 36 (12): 1202- 1205.
doi: 10.1016/j.tibtech.2018.07.016
5 王小理. 生物安全时代:新生物科技变革与国家安全治理[J]. 中国生物工程杂志, 2020, 40 (9): 95- 109.
6 孙琳, 杨春华. "美国优先"思维在生物安全领域的体现及影响[J]. 军事医学, 2019, 43 (2): 81- 88.
SUN Lin , YANG Chunhua . Embodiment and impact of "America First" strategy in the field of biosecurity[J]. Military Medical Sciences, 2019, 43 (2): 81- 88.
7 郑涛, 黄培堂, 沈倍奋. 当前国际生物安全形式与展望[J]. 军事医学, 2012, 36 (10): 721- 724.
ZHENG Tao , HUANG Peitang , SHEN Beifen . Status and prospect of global biosecurity[J]. Military Medical Sciences, 2012, 36 (10): 721- 724.
8 Jain S , Kamimoto L , Bramley AM , et al. Hospitalized patients with 2009 H1N1 influenza in the United States, April-June 2009[J]. N Engl J Med, 2009, 361 (20): 1935- 1944.
doi: 10.1056/NEJMoa0906695
9 Shi J , Deng G , Ma S , et al. Rapid evolution of H7N9 highly pathogenic viruses that emerged in China in 2017[J]. Cell Host Microbe, 2018, 24 (4): 558- 568.
doi: 10.1016/j.chom.2018.08.006
10 Yu XJ , Liang MF , Zhang SY , et al. Fever with thrombocytopenia associated with a novel bunyavirus in China[J]. N Engl J Med, 2011, 364 (16): 1523- 1532.
doi: 10.1056/NEJMoa1010095
11 Alaskar A , Shaheen NA , Bosaeed M , et al. Outcome of Middle East Respiratory Syndrome (MERS) in hematology and oncology patients: a case series in Saudi Arabia[J]. J Infect Public Health, 2020, 14 (3): 353- 357.
12 Xiao K , Zhai J , Feng Y , et al. Isolation of SARS-CoV-2-related coronavirus from Malayan pangolins[J]. Nature, 2020, 583 (7815): 286- 289.
doi: 10.1038/s41586-020-2313-x
13 Wacharapluesadee S , Tan CW , Maneeorn P , et al. Evidence for SARS-CoV-2 related coronaviruses circulating in bats and pangolins in Southeast Asia[J]. Nat Commun, 2021, 12 (1): 972.
doi: 10.1038/s41467-021-21240-1
14 Fang LQ , Sun Y , Zhao GP , et al. Travel-related infections in mainland China, 2014-16: an active surveillance study[J]. Lancet Public Health, 2018, 3 (8): e385- e394.
doi: 10.1016/S2468-2667(18)30127-0
15 Yao M , Jiang F , Zhang Y , et al. SARS-CoV-2 variant of concern 202 012/01 (B.1.1.7) in a Traveler from the United Kingdom to China[J].J Travel Med, 2021, 8, taab032.
doi: 10.1093/jtm/taab032
16 Normile D . Infectious diseases SARS experts want labs to improve safety practices[J]. Science, 2003, 302 (5642): 31.
17 Enserink M . Infectious diseases. Singapore lab faulted in SARS case[J]. Science, 2003, 301 (5641): 1824.
18 科学网.实验室SARS病毒泄漏事故回顾[ER/OL]. (2014-7-25) [2021-3-22]. http://news.sciencenet.cn/htmlnews/2014/7/299630.shtm.
19 王宇. 做好传染病防治人员安全防护提升传染病防控体系水平—中国疾控中心主任王宇解读《国务院办公厅关于加强传染病防治人员安全防护的意见》[J]. 首都公共卫生, 2015, 9 (2): 93- 94.
20 Centers for Disease Control and Prevention. Report on the inadvertent crosscontamination and shipment of a laboratory specimen with influenza virus H5N1 centers for disease control and prevention. [ER/OL]. (2014-08-15) [2021-3-22]. http://www.chinacdc.cn/zxdt/201409/t20140911_104332.htm.
21 Dawood FS , Iuliano AD , Reed C , et al. Estimated global mortality associated with the first 12 months of 2009 pandemic influenza A H1N1 virus circulation: a modelling study[J]. Lancet Infectious Diseases, 2012, 12 (9): 687- 695.
doi: 10.1016/S1473-3099(12)70121-4
22 David Grimm. Major coronavirus variant found in pets for first time [ER/OL]. (2021-03-19) [2021-3-22]. https://www.sciencemag.org/news/2021/03/major-coronavirus-variant-found-pets-first-time.
23 Garcia-Beltran WF , Lam EC , St Denis K , et al. Multiple SARS-CoV-2 variants escape neutralization by vaccine-induced humoral immunity[J]. Cell, 2021, S0092-8674 (21): 00298- 1.
24 Cello J , Paul AV , Wimmer E . Chemical synthesis of poliovirus cDNA: generation of infectious virus in the absence of natural template[J]. Science, 2002, 297 (5583): 1016- 1018.
doi: 10.1126/science.1072266
25 Noyce RS , Lederman S , Evans DH . Construction of an infectious horsepox virus vaccine from chemically synthesized DNA fragments[J]. PLoS One, 2018, 13 (1): e0188453.
doi: 10.1371/journal.pone.0188453
26 Kupferschmidt K. How Canadian researchers reconstituted an extinct poxvirus for $100, 000 using mail-order DNA [ER/OL]. (2017-07-07) [2021-3-22]. https://www.sciencemag.org/news/2017/07/how-canadian-researchers-reconstituted-extinct-poxvirus-100000-using-mail-order-dna.
27 Enserink M. Scientists brace for media storm around controversial Flu studies [ER/OL]. (2011-11-23)[2021-3-22]. https://www.sciencemag.org/news/2011/11/scientists-brace-media-storm-around-controversial-flu-studies.
28 Kaiser J. EXCLUSIVE: Controversial experiments that could make bird flumore risky poised to resume [ER/OL]. (2019-02-08) [2021-3-22]. https://www.sciencemag.org/news/2019/02/exclusive-controversial-experiments-make-bird-flu-more-risky-poised-resume.
29 Xie X , Muruato A , Lokugamage KG , et al. An infectious cDNA clone of SARS-CoV-2[J]. Cell Host Microbe, 2020, 27 (5): 841- 848.
doi: 10.1016/j.chom.2020.04.004
30 Xie X , Lokugamage KG , Zhang X , et al. Engineering SARS-CoV-2 using a reverse genetic system[J]. Nat Protoc, 2021, 16 (3): 1761- 1784.
doi: 10.1038/s41596-021-00491-8
31 Shan C , Xie X , Muruato AE , et al. An infectious cDNA clone of zika virus to study viral virulence, mosquito transmission, and antiviral inhibitors[J]. Cell Host Microbe, 2016, 19 (6): 891- 900.
doi: 10.1016/j.chom.2016.05.004
32 Xu M , Wang B , Deng F , et al. Establishment of a reverse genetic system of severe fever with thrombocytopenia syndrome virus based on a C4 strain[J]. Virol Sin, 2021, 15
doi: 10.1007/s12250-021-00359-x
33 Lee CW . Reverse genetics of influenza virus[J]. Methods Mol Biol, 2014, 1161, 37- 50.
doi: 10.1006/viro.2001.1008
34 Nogales A , Perez DR , Santos J , et al. Reverse genetics of influenza B viruses[J]. Methods MolBiol, 2017, 1602, 205- 238.
doi: 10.1007/978-1-4939-6964-7_14
35 Lee CW , Suarez DL . Reverse genetics of the avian influenza virus[J]. Methods Mol Biol, 2008, 436, 99- 111.
doi: 10.1007/978-1-59745-279-3_12
36 Liu Q , Dan H , Zhao X , et al. Construction and characterization of an infectious cDNA clone of coxsackievirus A 10[J]. Virol J, 2019, 16 (1): 98.
doi: 10.1186/s12985-019-1201-1
37 Ma YW , Hao SB , Sun LL , et al. Construction and characterization of infectious cDNA clones of enterovirus 71 (EV71)[J]. Virol Sin, 2015, 30 (4): 305- 308.
doi: 10.1007/s12250-015-3614-3
38 Ebina H , Misawa N , Kanemura Y , et al. Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus[J]. Sci Rep, 2013, 3, 2510.
doi: 10.1038/srep02510
39 Dupont L , Bloor S , Williamson JC , et al. The SMC5/6 complex compacts and silences unintegrated HIV-1 DNA and is antagonized by Vpr[J]. Cell Host Microbe, 2021, 29 (5): 792- 805.
doi: 10.1016/j.chom.2021.03.001
40 Langer S , Yin X , Diaz A , et al. The E3 ubiquitin-protein ligase cullin 3 regulates HIV-1 transcription[J]. Cells, 2020, 9 (9): 2010.
doi: 10.3390/cells9092010
41 Sullivan NT , Allen AG , Atkins AJ , et al. Designing safer CRISPR/Cas9 therapeutics for HIV: defining factors that regulate and technologies used to detect off-target editing[J]. Front Microbiol, 2020, 11, 1872.
doi: 10.3389/fmicb.2020.01872
42 Passos V , Zillinger T , Casartelli N , et al. Characterization of endogenous SERINC5 protein as anti-HIV-1 factor[J]. J Virol, 2019, 93 (24): e01221- 19.
43 Khabir M , Aliche AZ , Sureau C , et al. Hepatitis delta virus alters the autophagy process to promote its genome replication[J]. J Virol, 2020, 94 (4): e01936- 19.
44 Hatterschide J , Brantly AC , Grace M , et al. A conserved amino acid in the C terminus of human papillomavirus E7 mediates binding to PTPN14 and repression of epithelial differentiation[J]. J Virol, 2020, 94 (17): e0124- 20.
45 Lafaille FG , Harschnitz O , Lee YS , et al. Human SNORA31 variations impair cortical neuron-intrinsic immunity to HSV-1 and underlie herpes simplex encephalitis[J]. Nat Med, 2019, 25 (12): 1873- 1884.
doi: 10.1038/s41591-019-0672-3
46 Akidil E , Albanese M , Buschle A , et al. Highly efficient CRISPR-Cas9-mediated gene knockout in primary human B cells for functional genetic studies of Epstein-Barr virus infection[J]. PLoS Pathog, 2021, 17 (4): e1009117.
doi: 10.1371/journal.ppat.1009117
47 Tai-Schmiedel J , Karniely S , Lau B , et al. Human cytomegalovirus long noncoding RNA4.9 regulates viral DNA replication[J]. PLoS Pathog, 2020, 16 (4): e1008390.
doi: 10.1371/journal.ppat.1008390
48 Daniloski Z , Jordan TX , Wessels HH , et al. Identification of required host factors for SARS-CoV-2 Infection in human cells[J]. Cell, 2021, 184 (1): 92- 105.
doi: 10.1016/j.cell.2020.10.030
49 Kellen JS , Strtak AC , Sastri NP , et al. Rotavirus induces intercellular calcium waves through ADP signaling[J]. Science, 2020, 370 (6519): eabc3621.
doi: 10.1126/science.abc3621
50 Karakus U , Thamamongood T , Ciminski K , et al. MHC class Ⅱ proteins mediate cross-species entry of bat influenza viruses[J]. Nature, 2019, 567 (7746): 109- 112.
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