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Table of Content

      
    10 January 2013
    Volume 51 Issue 1
    Articles
    Effects of aspirin on the growth of murine sarcoma S180 and tumor angiogenesis and lymphangiogenesis in vivo
    ZHANG Xiao-yue1,2, ZHANG Wei-dong1, WANG Zhao-peng1, WANG Zhao-xia1, ZHANG Yue-ying1, JIA Qing1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(1):  1. 
    Abstract ( 964 )   PDF (5795KB) ( 431 )   Save
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    Objective   To investigate the effects of aspirin on the growth of murine sarcoma S180 and the mechanism of the inhibitory effect. Methods   40 mice were implanted with S180 cells and randomly divided into 4 groups: the control group, the 5-fluorouracil(5-FU) group, the high-dose aspirin group(250mg/kg) and the low-dose aspirin group(50mg/kg).The effect of aspirin on tumor growth was observed by recording tumor growth curve and calculating the inhibition rate. Pathological changes of the tumors were observed by HE stainning. Immunohistochemistry was applied to detect microvessel density(MVD), lymphatic vessel density(LVD), the levels of vascular endothelial growth factor-A(VEGF-A) and vascular endothelial growth factor-C(VEGF-C). Western blot was applied to detect the expressions of VEGF-A and VEGF-C. Results   High-dose and low-dose aspirin had inhibitory effects on the growth of murine S180 sarcoma (P<0.05). The inhibition rates of the high-dose and low-dose aspirin groups were 45.4% and 22.2% separately(P<0.05). The results of immunohistochemistry showed that the expressions of VEGF-A and VEGF-C were inhibited by aspirin. The MVD was decreased in a dose-dependent manner (P<0.05). The LVD was inhibited by 5-FU and aspirin especially in the high-dose aspirin group(P<0.05). Western blot results showed that the expressions of VEGF-A and VEGF-C were inhibited by aspirin. Conclusion   Aspirin can inhibit the growth of murine sarcoma S180. The mechanism might be associated with the inhibitions of tumor angiogenesis and lymphangiogeresis, which are due to down-regulated expressions of VEGF-A and VEGF-C.

    Inflammatory response promotes the growth of fibrosarcoma in the mice model
    BI Yu-li, HAN Ming-yong, XU Xiao-ya, JIANG Man, XU Ji-ying, SHUAI Xin-yan, HAN Jue-ming
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(1):  7-11. 
    Abstract ( 909 )   PDF (2174KB) ( 464 )   Save
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    Objective   To study the effects of inflammation on the growth of tumor in fibrosarcomabearing mice and the possible mechanism. Methods   C57BL/6 mice were randomly divided into 5 groups: the normal control group (PBS group), the lipopolysaccharide group (LPS group), the fibrosarcoma group (PC group), the LPS + fibrosarcoma group (LC group), and the LPS + fibrosarcoma + celecoxib group (Cele group). PBS buffer was intraperitoneally injected to the PBS group mice, and the remaining groups were given lipopolysaccharide (LPS) to establish the inflammation model. The mice of the LPS and PBS groups were killed on the fifth day, and the rest were subcutaneously inoculated with fibrosarcoma cells. Meanwhile the Cele group mice were given celecoxib and the mice were sacrificed 21 days after the inoculation. The general conditions and tumor volumes of the mice were compared. Mice were sacrificed, and the profile changes and the pathological characteristics of the lung tissues were studied. Tumor microvascular density and vessel phenotype of PC group and LC group mice were compared by CD31 staining. Results   Compared with the PBS group, the lung vascular permeability and the tissue fluid exudation increased, and a large number of red blood cells and inflammatory cells were observed in the LPS group. Compared with the PC group, the tumors in the LC group grew faster, the microvascular density increased and the blood vessels were in disorder. Conclusion   The inflammatory response promotes the growth of fibrosarcoma possibly by the promotion of the tumor angiogenesis. Antiinflammatory treatment can inhibit the growth of fibrosarcoma.

    Qin Dan capsule inhibits ventricular hypertrophy in spontaneously hypertensive rats
    YAN Lei1, ZHANG Ji-dong1, HUANG Shan-ying2, WANG Bo1, REN Min1, QIAO Yun1, L Yi-jing1, GUO Xue-feng1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(1):  12. 
    Abstract ( 779 )   PDF (3996KB) ( 459 )   Save
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    Objective   To observe the effects of Qindan Capsule (QD) on inhibition of ventricular hypertrophy and the expression of NF-κB in spontaneously hypertensive rats (SHR) by way of activating PPARγ so as to investigate the mechanism of QD reversing hypertension ventricular hypertrophy. Methods   Spontaneously hypertensive rats(SHR)were divided into the model group(SHR group), QD high dosage group(SHR+QDH group), QD low dosage group(SHR+QDL group), and SHR+Telmisartan group(SHR+Tel group). Wistar-Kyoto group(WKY)rats of the same weeks were taken as the normal control(WKY group). All rats were administered with corresponding medicine or normal saline. Animal blood pressures were measured by tail-cuff plethysmography.Morphological changes of myocardial tissue were evaluated by HE staining. Ultramicrostructural changes of myocyte were determined by transmission electron microscopy. Expressions of PPARγ and NF-KB were detected by immunohistochemical staining. Results   Compared with those of the SHR group, the systolic blood pressures and the myocardial expressions of NF-κB in the SHR+QDH group, SHR+QDL group and SHR+Tel group were significantly reduced by QD or Tel (P<0.05). In the contrast, the myocardial expressions of PPARγ in the SHR+QDH group, SHR+QDL group and SHR+ Tel group increased by QD or Tel compared to those of the SHR group (P<0.05). The extent of ventricular hypertrophy in the SHR+QDH group was drasticly less than that in the SHR+QDL group(P<0.05). Conclusion   QD ameliorates the ventricular hypertrophy by enhancing the PPARγ expression so as to suppressing the NF-KB expression in myocyte.

    Influences of pioglitazone on the appearance of protein tyrosine phosphatase 1B in the pancreas of high fat diet induced insulin resistance rats
    YU Su-guo1, WANG Ling-ling2, SUN Ji-hua3, WANG Ying-xue1, ZHAO Hui1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(1):  17-21. 
    Abstract ( 719 )   PDF (2094KB) ( 437 )   Save
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    Objective   To observe the effect of Pioglitazone on the appearance of protein tyrosine phosphatase 1B in the pancreas of insulin resistance rats. Methods   60 SD rats were randomly divided into the control group (NC group, n=10) on regular diet, the high fat diet group (HFN group, n=15) and the high fat diet group administrated with Pioglitazone (HFP group, n=15). The last two groups (HF groups) were on high fat diet. After twelve weeks, verify the features of the models. The HFP group were treated with Pioglitazone [30mg/(kg·d), intragastric administration] daily for 2 weeks. The NC group and the HFN group received a saline intragastric administration of equal volume. The expression of PTP-1B in the pancreas of rats was detected by immunohistochemistry and Western blot. Results   The sensitivity of insulin was degraded in the HF group than that in the NC group (P<0.01). The immunohistochemistry and Western blot test showed that the expression of PTP-1B protein in the HF group was higher than that in the NC group (P<0.01). The expression of PTP-1B protein in the HFP group significantly decreased compared with that in the HFN group (P<0.01). Conclusion   The obese rats induced by high fat diet show insulin resistance. At the same time, the PTP-1B level in their pancreas increase. Pioglitazone could degrade the expression of PTP-1B in the pancreas and improve the insulin resistance.

    Changes of BDNF after treatment of traumatic brain injury with human umbilical cord blood mesenchymal stem cells in rats
    CHEN Nai-yao1, SHEN Na2, HE Jing-an3, ZHENG Li-kun4
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(1):  22. 
    Abstract ( 850 )   PDF (4854KB) ( 378 )   Save
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    Objective   To study the potential mechanism for treatment of traumatic brain injury with human umbilical cord blood mesenchymal stem cells (HUCBMSCs) in rats. Methods   HUCBMSCs were cultured in vitro and labled with 5-bromo-2-deoxyuridine(Brdu). Sprague-Dawley(SD)rats which suffered from traumatic brain injuries were randomly divided into 3 groups (30 rats in each group): sham-operated group,  control group and treatment group which was injected intravenously with the labled HUCBMSCs. The neurological severity score(NSS) were evaluated in control group and treatment group. Additionally, brain-derived neurotrophic factor (BDNF) was determined at protein level by immunohistochemistry and at mRNA level by in situ hybridization. Results   ①There was no Brdu-labeled cells in sham-operated group and control group. However, Brdu-labeled cells gathered obviously in the damage surrounding area in treatment group and the labeled cells decreased gradually with the time prolonged; ② NSS was obviously improved in treatment group since day 7 after the adminstration of HUCBMSCs; ③There were only a minimal number of nerve cells expressing BDNF in the sham-operated group. Both of control and treatment group showed remarkable increases of BDNF expression which reached a peak at day 14 after injection of HUCBMSCs and then declined gradually. However, BDNF expression increased significantly in treatment group, compared with control group at same time points. Conclusion   HUCBMSCs can survive for at least 4 weeks in brain tissues without application of immunosuppressants in rats. The intravenously injected HUCBMSCs which distribute in the damage surrounding area are more than in other areas. The current study indicates that the brain demage is improved after HUCBMSCs injection and the enhanced BDNF expression benefits in the repair of demaged brain tissues.

    Effect of medical ozone on chondrocytes in the pathological process of osteoarthritis 
    FAN Rong1,2, FU Zhi-jian1,2, XIE Jun-tian1, SHI Cheng-ying1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(1):  27. 
    Abstract ( 898 )   PDF (2236KB) ( 276 )   Save
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    Objective   To observe the effects of different concentrations of medical ozone(O3) on damaged articular cartilage cells induced by interlukin-1β(IL-1β), and discuss the rational concentration boundry of O3 in the clinical practice. Methods   Cartilaginous layers of knee were separated from seven-month old NewZealand rabbit under semi-asepsis environment and then digested under sterile environment to get the cartilage cells. The cartilage cells were cultured and subcultured after digested under sterile environment. The experimental cells were the third generation cartilage cells. Chondrocytes were divided randomly into eight groups cultured with different mediums: the normal chondrocytes group, chondrocytes induced by IL-1βas the model cell group, chondrocytes with O3(20, 40, 60μg/mL) as the control groups, chondrocytes induced by IL-1β with O3(20, 40, 60μg/mL) as the experimental groups. After 24 hours, the cells morphology was observed. Malondialdehyde (MDA), tumor necrosis factor-α (TNF-α)and matrix metalloproteinase-3 (MMP-3) content in the cartilage cell culture fluid were detected by enzyme-linked immunosorbent assay (ELISA). Results   The shape of the cells in the experimental group, the control group and the normal cartilage cells group was comparable, after induced by 20, 40μg/mL concentrations of O3. A few cartilage cells induced by low and intermediate concentration of O3 showed pyknosis, and the levels of MDA, TNF-αand MMP-3 in the cartilage cell culture fluid were significantly lower than those of the model group (P<0.05). Cartilage cells induced by 60μg/mL concentration of O3, were seriously damaged, even showed apoptosis. MDA, TNF -αand MMP-3 content in the cartilage cell culture fluid was significantly higher than that of the model group (P<0.05). Conclusion   The groups of 20, 40μg/mL concentration of O3 have a protective effect by lowing concentration of inflammatory factors significantly in chondrocytes of osteoarthritis. The 60μg/mL concentration of O3 damages either the cartilage cells in the pathological process of osteoarthritis or normal cartilage cells.

    Treatment effect of paclitaxel loaded microspheres in Skov3 tumor-bearing nude mice
    JIANG Xin1, GUO Wen-bin1, ZHU Mei1, LI Qiao1, JIANG Ting2, ZHANG Shuo1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(1):  33-36. 
    Abstract ( 1019 )   PDF (28060KB) ( 179 )   Save
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    Objective   To prepare PLGA [poly (lactic-co-glycolic acid)] as the sustained-releasing microspheres loaded with paclitaxel and to evaluate the treatment effect on the implanted tumor induced by Skov3 cells in the nude mice. Methods   Four-week-old female BALB/c-nu mice were used as tumor models by injecting Skov3 cells at the back side, and they were evenly divided into four groups. And inner tumor injection was done by using physiological saline, the PLGA microspheres, the paclitaxel and the paclitaxel loaded PLGA microspheres separately. Then the mice were treated and observed and the tumor growth curves were recorded.Direct inner tumor injection was done and the MVD and Bad was measured by immunohistochemistry. Results   After 6 weeks of treatment, tumor size and volume of tumor models treated with PLGA sustained-releasing microspheres loaded with paclitaxel were significantly decreased, compared with those of other groups(P<0.05). Conclusion   PLGA sustained-releasing microspheres loaded with paclitaxel by direct inner tumor injection has a remarkably inhibitory effect on ovarian cancer.

    Experimental study of bletilla glucomannan/nacre powder composites repairing the alveolar bone defects in rabbits
    PANG Yong-zhi1, HU Wen-ting2, PENG Feng-mei3
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(1):  37. 
    Abstract ( 915 )   PDF (2091KB) ( 417 )   Save
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    Objective   To observe the repair effect and the degradation and absorption of the bletilla glucomannan/nacre powder composites in application of the rabbit alveolar bone’s local defect. Methods   60 rabbits were divided into 3 groups. GroupⅠ: defect was filled with bletilla glucomannan / nacre powder composites; Group Ⅱ: defect was filled with nacre powder; Group Ⅲ: defect was not filled with any implants. The effects were observed by imaging, bone mineral density(BMD), gross anatomy, histological, and alkaline phosphatase(ALP) at 1,4,8,12 weeks after surgery. Results   The nacre powder and bletilla glucomannan had a good biocompatibility. Implant material of the three groups had an entirely degradation at 12 weeks after surgery. Group Ⅰ had a better osteogenesis ability than group Ⅱ and Ⅲ. The BMD(0.411±0.0188)g·cm-2of group Ⅰ was greater than that of group Ⅱ and the BMD of group II was greater than that of group Ⅲ at 12 weeks after surgery(P<0.05). At the same postoperative time points, the ALP activity of the group Ⅰwas significantly greater than that of group Ⅱ and Ⅲ. Conclusion   Chinese medicine bletilla glucomannan/pearl powder composites can repair partial alveolar bone defect quickly, and open up a new direction for Chinese medicine treatment of alveolar bone defect.

    Effect of PTEN transfection on human tongue squamous carcinoma cell line SCC-4 and its mechanism
    LIU Shuo-shuo, ZHANG Bin, GUO Ting-ting, DONG Xiao-ting
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(1):  42-45. 
    Abstract ( 872 )   PDF (1552KB) ( 186 )   Save
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    Objective   To explore the impact of PTEN transfection on the apoptosis of human tongue squamous carcinoma cell line SCC4 and its mechanism. Methods   Cells transfected with PTEN (pEGFP-PTEN-SCC-4) were set as the experimental group. Cells transfected with pEGFP-SCC-4 and normal SCC-4 were the control groups. The flow cytometry was used to detect the apoptosis of three groups. The expressions of Akt, p-Akt and Bim proteins were detected by Western blotting. Results   The apoptosis rate of the experimental group was significantly higher compared with the control groups(P<0.01). Western blotting showed there was no significant difference of the total Akt level between three groups. The expression of p-Akt was significantly decreased in the experimental group compared with the control groups, and the level of Bim was higher in the experimental group compared with the control groups. Conclusion   PTEN transfection may induce the apoptosis of human tongue squamous cell SCC-4 by down regulating PI3K/Akt signaling pathway and increasing Bim expression.

    Data analysis for the positive rate of selective coronary artery angiography and the risk factors in coronary artery stenosis
    FU Shen-shen1, LI Da-qing1, LI Ji-fu1, LIU Bin2, YANG Min1, SHAO Na1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(1):  46. 
    Abstract ( 859 )   PDF (1179KB) ( 325 )   Save
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    Objective   To evaluate the detection rate of coronary stenosis with coronary angiography (CAG) and analyze the coronary risk factors associated with the coronary stenosis, in order to provide a basis for increasing the CAG-positive detection rate. Methods   The data of patients with CAG test collected from March 2008 to December 2008 in Qilu Hospital of Shandong University were analyzed to observe the positive rate of LM, LAD, LCX and RCA. Chi-square test was used in the categorical data. Multinomial logistic regression analysis was used to evaluate the risk factors related to the coronary stenosis. A discriminate predicting score and receiver operating characteristic curve (ROC) analysis were performed to evaluate the predictive diagnostic sensitivity and specificity. Results   The diameter of coronary artery stenosis (CAS) > 25% was diagnosed as the positive event, and the positive rate of CAG was 64.21%. The positive rate of men with age ≤ 59 was 56.19%, and 32.47% for women with age ≤ 59. The negative rate of women was higher compared with the men. The risk factors associated with CAS were gender, age, diabetes, hypertension, smoking and the blood biochemical indicators including A/G, TBA, TG, HDL, GLU, Cr, FIB and HGB (P<0.05). The sensitivity and specificity were 54.40% and 89.90% based on the predicting score in the discriminate regression and ROC analysis. Conclusion   The risk factors associated with CAS can enhance the detection rate of coronary artery stenosis (CAS).

    Prediction of ankle-brachial index to all-cause and cardiovascular mortalities in patients with high cardiovascular risk
    TANG Hai-rong, YUAN Bin-bin, HU Da-yi, Buaijiaer·Hasimu
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(1):  53. 
    Abstract ( 849 )   PDF (923KB) ( 280 )   Save
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     Objective   To assess the correlation between ankle-brachial index (ABI) and 1-year all-cause and cardiovascular mortalities in patients with high cardiovascular risk. Methods   3179 patients with high cardiovascular risk were selected. The ABI indexes at resting state were detected, and ABI<0.9 was regarded as low ABI. The patients were divided into four groups: coronary atherosclerotic heart disease (CHD) group, ischemic stroke (IS) group, diabetes mellitus (DM) group and very high risk (VHR) group. Then the patients were followed up for 1 to 1.5 years, and the correlations between ABI and 1-year all-cause and cardiovascular mortalities were assessed. Results   The incidence of low ABI was 28.2%. The all-cause and cardiovascular mortalities (14.2%, 6.6%) in patients with low ABI were obviously higher than those in patients with normal ABI (6.5%, 2.5%) (P<0.01). In each group, the all-cause and cardiovascular mortalities in patients with low ABI were obviously higher than those in patients with normal ABI(P<0.01). The all-cause and cardiovascular mortality risks in DM group were higher than those in the other three groups. Conclusion   ABI is an independent predictor of mortality, and patients with low ABI have a substantial increase in the risk of all-cause and cardiovascular death.

    Meta-analysis of drug-eluting stents implantation versus vascular brachytherapy for in-stent restenosis
    FANG Hai-yang, XIANG Jian ,WU Yan-qing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(1):  56-63. 
    Abstract ( 817 )   PDF (1392KB) ( 300 )   Save
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    Objective   To compare the efficacy and safety of drug-eluting stents(DES) implantation and vascular brachytherapy(VBT) for in-stent restenosis(ISR). Methods   Controlled trials of DES versus VBT for in-stent restenosis were collected by literature search method and Meta-analysis was performed by using Review Manager software version 5.1. Results   13 controlled trials with 2312 patients were included. Of the 2312 patients, 1259 were in the DES group and 1053 were in the VBT group. Meta-analysis indicated that, the incidence of binary restenosis (P<0.00001), late lumen loss (P<0.00001), target-vessel revascularization (TVR)(P=0.03) and adverse cardiac event (MACE)(P=0.004) in the DES group were significantly lower compared with those of the VBT group. There were no significant differences in the incidence and mortality of late stent thrombosis between the two groups. Conclusion   Drug-eluting stents are markedly superior to VBT for the treatment of ISR in binary restenosis, late lumen loss,TVR reduction, but not in late stent thrombosis and mortality reduction.

    Relevant factors of postoperative complications for hepatectomy combined with splenectomy
    HU Liang, YI Yong-xiang, DING Hai, HAN Jian-bo, LIU Tao, ZHAO Liang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(1):  64-66. 
    Abstract ( 773 )   PDF (876KB) ( 296 )   Save
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    Objective   To analyze the relevant factors of postoperative complications for hepatectomy combined with splenectomy. Methods   68 patients with hepatocelluar carcinoma and portal hypertension who underwent hepatectomy and splenectomy were reviewed. The relevant factors of postoperative complications were analyzed.  Results   The postoperative complication rate was 32.4%(22/68). The main complications consisted of the splenic recess  and pleural effusion, infection of the effusion, bile leakage and so on. There was no relevance between the postoperative complication rate and the age, sex, serum AFP, HBV-DNA quantity and tumor location(P>0.05). The postoperative complication rate was positively correlated to the tumor size, Child-pugh classification and severe degree of liver cirrhosis(P<0.05). Conclusion   It can reduce complications after hepatectomy combined with splenectomy by perioperative management to improve liver function and liver functional reserve.

    Expressions of Ki-67, PCNA and Survivin in gastrointestinal stromal tumor tissues
    ZHANG Yan-xia1, ZHANG Pei-jun2, YAN Yan-yan3, DUAN Ai-xu4, LI Jia-shan2, NIU San-qiang5
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(1):  67-70. 
    Abstract ( 713 )   PDF (897KB) ( 297 )   Save
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    Objective   To investigate expressions of Ki-67, PCNA and Survivin in the gastrointestinal stromal tumor(GIST) tissues and the relationship with clinic pathological factors. Methods   The SP immunohistochemical assay was used to detect the expressions of Ki-67, PCNA and Survivin in 103 cases with GIST. Results   The Ki-67, PCNA and Survivin positive expression rates were 56.3%, 68.0% and 72.8%. There was no correlation between Ki-67, PCNA, Survivin expressions and the age, sex,  location and histological type. The expressions of Ki-67, PCNA and Survivin were related to the NIH grade. There were significant positive correlations among Ki-67, PCNA and Survivin expressions. Conclusion   Ki-67,  PCNA and Survivin can be used as the predictor for the malignant degree and prognosis of GIST.

    Observation of the reflux characteristics of reflux esophagitis in patients with different body mass indexes using multichannel intraluminal impedance
    SONG Xuan, HAO Hong-sheng, L Guo-ping, SUN Li-juan, LU Xiao-heng
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(1):  71-74. 
    Abstract ( 855 )   PDF (895KB) ( 292 )   Save
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    Objective   To observe the reflux characteristics of patients with reflux esophagitis in different body mass indexes using combined multichannel intraluminal impedance-pH (MII-pH) monitoring.  Methods   56 patients with reflux esophagitis were enrolled and divided into the normal group, overweight group and obesity group according to their body mass indexes. We observed reflux-related indicators of individuals with MII-pH monitoring, and then performed statistical analysis on pH monitoring and impedance results. Results   Compared with normal group, there were significant differences in overweight group and obesity group on percent time pH<4 (total, upright and recumbent), acid reflux and liquid reflux (P<0.05 or 0.01),while no significant differences were found between overweight and obesity groups (all P>0.05). There were no significant differences in weakly acid reflux, non-acid reflux, mixed reflux, gas reflux and proximal reflux episodes among the three groups (all P>0.05). Conclusion   There are more acid exposure, acid reflux and liquid reflux episodes in overweight and obese patients; however, the effect of the extent of obesity on acid exposure, acid reflux and liquid reflux has no significant difference.

    Clinical significance of ultrasoundguided percutaneous nephrostomy
    LIU Cong-cong, XIAO Jing, SHI Dan-dan, LIU Yan, LI Hai-ying, ZHOU Wei, ZHANG Kai-ning, WANG Meng, LI Jie
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(1):  75-78. 
    Abstract ( 901 )   PDF (1096KB) ( 385 )   Save
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    Objective   To investigate the clinical significance and experience of ultrasound-guided percutaneous nephrostomy (PCN). Methods   Ultrasound-guided PCN was performed on 71 patients with hydronephrosis. 11 patients of them underwent bilateral ultrasound-guided PCN. The clinical efficacy of PCN was observed and analyzed. The renal function of the patients was checked and the urine volume was observed 1 week before and 1 week after percutaneous nephrostomy. Results   Totally 82 PCN procedures were performed on the 71 patients with the primary successful rate of 96.34% (79/82). The renal function of all the patients was improved greatly in 1 week. No one had severe complications or died due to operation complications. Conclusion   Ultrasound-guided PCN is claimed to be a safe, simple, effective procedure with almost no serious complications. This technique has important clinical significance because it can improve renal function definitely.

    Clinical observation on intravesical instillation of sodium hyaluronate for treatment of non-bacterial cystitis
    ZHANG De-qing, ZHU Yao-feng, MENG Hui, WANG Jun, LI Yan, SHI Ben-kang
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(1):  79-82. 
    Abstract ( 1004 )   PDF (891KB) ( 556 )   Save
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    Objective   To observe the clinical efficacy and safety on intravesical instillation of sodium hyaluronate for treatment of non-bacterial cystitis. Methods   Totally 28 patients with non-bacterial cystitis were included. They received intravesical instillation(sodium hyaluronate solution of 50ml/40mg) once a week for 4 weeks, then once every two weeks for the next 4 weeks and at last once a month for the next 2 months. The follow-up visited was performed once every two weeks, ended at the 16th week. Evaluate the efficacy and safety of the sodium hyaluronate according to patients’ symptom assessment scores(VAS, LUTS) and the micturition diary. Results   28 patients all completed the instillation and follow-up. At the 16th week, the VAS score dropped from 6.86±1.74 to 2.71±0.90(P<0.001), the LUTS score dropped from 41.07±11.96 to 23.25±6.07(P<0.001) and the mean BC(bladder capacity) rose from (106.71±22.65)mL to (163.93±32.00)mL(P<0.001). Conclusion   The short-term curative effect and safety of intravesical instillation of sodium hyaluronate to treat the non-bacterial cystitis is worth affirmation. It can significantly relieve symptoms in order to improve the quality of life.

    The correlation between features of DTI and JOA scores in patients with cervical spinal cord injury without radiographic abnormality
    LI Xu, QIN Dong-jing, CAO Xin-shan, JIANG Xing-yue, ZHANG Di, WANG Jing
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(1):  83-87. 
    Abstract ( 1051 )   PDF (2958KB) ( 450 )   Save
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    Objective   To observe the correlation between features of diffusion tension imaging (DTI) and Japanese Orthopaedic Association (JOA) scores in patients with cervical spinal cord injury without radiographic abnormality (CSCIWORA). Methods   31 CSCIWORA patients were assessed and divided into 4 stages according to JOA scores. The score of stage Ⅰ/Ⅱ/Ⅲ/Ⅳ was 16-13, 12-9, 8-5 and less than 4 respectively. All the patients accepted conventional scan and DTI. The Fractional Anisotropy (FA) and Apparent Diffusion Coefficient (ADC) were measured respectively, and the correlation to JOA scores was observed. Results   Stage Ⅰ: FA was slightly lower, ADC was slightly higher; Stage Ⅱ: FA was lower, ADC was higher; Stage Ⅲ: FA was significantly lower, ADC was confounding; Stage Ⅳ: FA was the lowest, ADC was lower. FA was related to JOA score of patients with CSCIWORA(P=0.000 ), ADC was not evidently related to JOA scores of patients with CSCIWORA(P=0.802 ). Conclusion   DTI could show the pathophysiology process of CSCIWORA more directly and more efficiently. It is suggested that DTI can provide valuable information about injury degree of cervical spinal cord and prognosis evaluation.

    Clinical effect of 32P applicator combined with needleless skin instrument in the treatment of chest keloid
    LI Zhong-hua1, WANG Hai-qin2, WANG Yi-min1, YANG Bo1, SUN Yu-sheng1
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(1):  88-92. 
    Abstract ( 914 )   PDF (1342KB) ( 458 )   Save
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    Objective   To investigate the clinical efficacy of 32P applicator combined  with needleless skin instrument for treating chest keloid. Methods   Of all the 180 chest keloid patients, 60 with lesion thickness ≤0.2cm were randomly divided into three groups: 20 received compound betamethasone injection therapy, 20 received 32P applicator therapy, others were treated with 32P applicator combined with needleless skin instrument. 120 ones with lesion thickness >0.2cm were randomly divided into four groups: 30 received compound betamethasone injection therapy, 30 received 32P applicator therapy, 30 received operation combined with 32P applicator therapy, others were treated with operation combined with 32P applicator and needleless skin instrument. Results   ① The cure rate and effective rate in the compound betamethasone injection group were 35.0% and 55.0%, which were significantly lower than those(75.0%, 85.0%)in the simple 32P application group and (80.0%, 90.0%)in the 32P applicator combined with needleless skin instrument group(P<0.05); ② The cure rate and effective rate in the compound betamethasone injection group were 3.3% and 23.3%, which were significantly lower than those in the other groups(P<0.05); The cure rate and effective rate in the simple 32P application group were 20.0% and 53.3%, which were significantly lower than those(86.7%, 96.7%) in the operation combined with 32P applicator and needleless skin instrument group and in the operation combined with 32P applicator group(P<0.05); The cure rate in the operation combined with 32P applicator and needleless skin instrument group was significantly higher than that in the operation combined with 32P applicator group(P<0.05). Conclusion   For patients with lesion thickness ≤0.2cm, 32P applicator therapy only is enough. Otherwise, patients are suggested to use operation combined with 32P applicator and needleless skin instrument therapy.

    Mutation screening of candidate genes causing disseminated superficial porokeratosis
    LIU Chen-fan1, WANG Zhen-ying2, SONG Huai-dong3, PAN Chun-ming3, ZHANG Li2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(1):  93-97. 
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    Objective   To identify the candidate genes causing disseminated superficial porokeratosis(DSP) by large-scale mutation screening and analysis of the located segments. Methods   We collected the pedigree members’ clinical data and blood samples. Afterwards the genomic DNA of peripheral blood and the RNA of the skin tissue from healthy persons were extracted. First of all, we processed RT-PCR with RNA by classical method. Then, we designed PCR primers and selected the genes expressed in the skin to performe PCR amplification with genomic DNA of individuals of the pedigree. Afterwards, the PCR products were purified and sequenced with 2μL purified product. The mutation analysis was performed for the sequence in order to find the disease gene causing DSP. Results   We found 24 genes were expressed in the candidate region between D12s1671 to D12s1605 by RT-PCR.  We screened 56 candidate genes. A c.4242T>G nonsynonymous mutation in UHRF1BP1L gene that resulted in a substitution phenylalanine for leucine at codon 1414 (p.F1414L) and a c.2487G>A synonymous mutation at the codon 829 in ANO4 gene, were found in all of the patients in the DSP family. Conclusion   2 SNPs are found in all of the patients in the DSP family and 56 candidate genes are excluded.

    Epidemiological investigation of hypertensive retinopathy in residents of Ningyang county in the Shandong province
    LI Ying-mei1,2, WU Xin-yi1, WANG Yu2, FAN Chuan-feng2, SHEN Yan-juan2, SHU Xiang-wen2, CHEN Xuan2, WU Chang-long2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(1):  98-102. 
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    Objective   To explore the morbidity of hypertensive retinopathy (HR)in the residents of Ningyang county in the Shandong province and to analyze its related risk factors. Methods   Some residents over 35 years old in the Ningyang county were selected by a cluster random sampling method and investigated with questionnaire surveys, physical  examination  and venous blood tests. Results   1086(81.78%) hypertension patients completed all of the questionnaire, physical examination and laboratory detection. There were 313 patients with normal retinal and 783 with hypertensive retinopathy in varying degrees, including 532 cases of gradeⅠ(68.82%),159 cases of grade Ⅱ(20.57%), 79 cases of grade Ⅲ (10.22%) and none of grade Ⅳ, and  only  53 cases occurred ocular complications (6.86%). The morbidity of HR in Ningyang county was 71.18%. 859 patients knew that they were suffering from high blood pressure diseases (79.1%) and 342 had been treated with drug therapy (31.5%). The morbidity of HR in the rural residents was 73.68%, which was clearly higher than that in county residents (64.82%). With the increase of patient’s age, blood pressure value and the course of the  disease, the morbidity of HR was increased, but there was no significant correlation with the gender of the patients. By ordinal logistic regression analysis, age, rural area, and the course of hypertension were risk factors of HR. The severity of HR in patients with mix-type hypertension(sbp≥140 and dbp≥90) was 2.44 times than that of the normal blood pressure group. The severity of HR with the group over 10-years hypertension course was 4.15 times than that of the group of the course <1 year. Conclusion   The morbidity of hypertensive retinopathy in Ningyang county is relatively high. The risk factors include blood pressure, age, residential area,and the course of hypertension. And it is characterized with low awareness rate , low treatment rate and low control rate. Ophthalmologists need close cooperation with medical institutions at all levels to establish  the  prevention and control system of HR.

    Associations of chromosomes 17q22, 10q25.3 and ABCA4 gene polymorphisms with nonsyndromic cleft lip/palate in Ningxia Hui and Han population
    ZHOU Zhong-wei1, YANG Xiong1, WAN Ying-biao2, XIN Yan-hua1, ZHAI Kun1, MA Jian2, HUANG Yong-qing2, JIANG Min3, WANG Yi-rui4
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(1):  103-108. 
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    Objective   To explore if genetic variation in chromosome 17q22(rs22731),ABCA4 (rs560426) and 10q25.3(rs7078160)genes contributes to non-syndromic cleft lip with or without cleft palate (NSCL/P) in Ningxia Hui and Han population. Methods   The study consisted of 415 NSCL/P patients(Han=191,Hui =224)and their parents (303 fathers and 311 mothers, including 158 complete families). They were divided into 3 groups: the cleft lip only(CLO), the cleft palate only(CPO) and both the cleft lip and palate(CLP)  groups, and we merged the CLO and CLP groups into the cleft lip with or without palate (CL/P) group.  In addition, 385 healthy  college students (Han=261,Hui =104)consisted of the control group. The single nucleotide polymorphisms(SNPs) were genotyped by TaqMan SNPs genotyping and the data were analyzed by case-control analysis, transmission disequilibrium test and family based association test. Results   ① The distribution of alleles at rs227731 on chromosome 17q22 was significantly different between CLO and control groups in the Hans (P<0.05), and significant difference was found between CL/P and control groups in the Hans (P<0.05). There were significant differences in the genotypic and allelic frequencies of CLP and CL/P groups between the two ethnics. ② The distributions of both genotypes and alleles at rs560426 in ABCA4 gene were also significantly different between CLP and control groups in the Hans (P<0.05), and significant difference was found between CL/P and control groups in the Hans (P<0.05). There were significant differences in the genotypic and allelic frequencies of CLO groups between the two ethnics. ③ No differences were found in genotypic and allelic frequencies of rs7078160 on chromosome 10q25 between the patients and their parents and the controls, and between the two ethnics. Conclusion   rs227731 and rs560426 SNPs are associated with NSCL/P in Ningxia Han population, and rs7078160 SNPs are not associated with NSCL/P in Ningxia population.

    The retrospective analysis of 53 patients with bisphosphonate-related osteonecrosis of the jaw
    LI Yin-zhu1, XU Wen-jun2, CUI Jing-ying2, REN Cui-ai2
    JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2013, 51(1):  109-112. 
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    Objective   To explore the clinical manifestations and treatments of bisphosphonate-related osteonecrosis of the jaw. Methods   The clinical manifestations、 diagnostic and treatments of 53 multiple myeloma cases of bisphosphonate-related osteonecrosis of the jaw admitted to our hospital between 2005-2011 were analyzed retrospectively. Results   53 patients were all treated with antibiotics. 12 patients of them were performed with bone debridement/palliative surgeries, 12 patients were performed with bone debridement/palliative surgeries in association with ozone treatment and 14 patients were performed with ozone treatment. 26 patients were performed in association with ozone treatment,27 patients weren’t performed in association with ozone treatment.The efficient treated with antibiotics alone was 60%, the antibiotic therapy in association with surgeries was 58.4%, in association with ozone treatment was 92.3%, no ozone treatment was 59.2%. Conclusion   Antibiotics can control disease progression in BRONJ patients, the remission rate treated by antibiotics combined with surgeries is not significantly higher than that treated by antibiotics alone, and will affect the quality of life of patients. We recommend conservative treatment in BRONJ patients.In addition, ozone treatment, compared with other treatments, has significantly higher efficiency, so its clinical application is proposed.