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Lifestyle medicine Xin MA Journal of Shandong University (Health Sciences)    2020, 58 (10): 1-6, 12.   DOI: 10.6040/j.issn.1671-7554.0.2020.1151 Abstract （8834）   HTML （0）    PDF（pc） （1552KB）（97）       Save Lifestyle medicine (LM), an emerging new medical discipline, uses evidence-based lifestyle therapeutic approaches (non-drug, non-surgery modalities) to prevent, treat and oftentimes reverse lifestyle-related chronic disea- ses. Board Certification of LM was first initiated by American Society of Lifestyle Medicine in 2017 and followed by countries in Europe and Asia. LM has been considered a global movement and the future of healthcare reform. "Healthy China 2030 Plan" and " Healthy China Action Plan" issued by China government in 2016 and 2019 respectively have emphasized the importance of "people?s healthy lifestyle" and "everyone as the first responder of his/her own health". The establishment and development of LM in China will play a significant role in the "Healthy China 2030 Plan" and be an indispensable component of "Healthy China Action Plan". Reference | Related Articles | Metrics

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Assessing the general mental health of college students:psychometric properties of GHQ-28 CHEN Cui, ZHANG Hongjing, JIANG Hong, LI Wenjie, Lv Li JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2010, 48 (3): 159-162.   Abstract （8338）      PDF（pc） （330KB）（993）       Save Objective   To check the psychometric properties of GHQ-28 in college students. Methods   Three questionnaires, GHQ-28, SAS, and SDS, were used to assess 248 college students. Then the item-analysis was used to analyze each item of the GHQ-28,  the Cronbach′s α of the GHQ-28 was checked,  factor analysis was used to check the structure validity of the GHQ-28 and the SAS and SDS was applied to check the criterion validity of the GHQ-28. Results    The correlations between each item of the GHQ-28 and its scales were positive (P<0.01). The reliabilities of the whole scale and the part scales were 0.872, 0.767, 0.667, 0.756 and 0.700. The GHQ28 contains four factors, and the aberration rate of them was 48.381% of the total. The GHQ-28 factors′ levels of students with high scores in SAS were higher than those low in SAS, as well as in SDS (P<0.001). The correlations of the GHQ-28 and SAS, SDS were 0.707 and 0.749. Conclusion    The GHQ-28 has good reliability and validity when it measures the general mental health of college students. Related Articles | Metrics

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Screening of molecular markers for epidermal stem cells in mice by the double immunofluorescence labeling technique CHEN Meng1, REN Ning2, LIU Wen-jun1, QIN Xiao-min1, LI Jin-song1 JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2011, 49 (4): 65-69.   Abstract （7717）      PDF（pc） （1781KB）（403）       Save Objective    To screen reliable molecular markers for epidermal stem cells(ESCs) in mice by using the double immunofluorescence labeling technique and labelretaining cells(LRCs). Methods     The epidermis was labeled for 7 days with 5-bromodeoxyuridine(BrdU) in postnatal day 5(P5) mice, followed by a trace period of up to 12 weeks. The tissue of the mice was frozen and sliced in a frozen microtome after 90 days. Slices were stained with polyclonal antibodies of anti-β1 integrin, P63 and CD34 and monoclonal antibodies of anti-Brdu, and then observed under a fluoroscope. Results    After 90 days, few LRCs were found in a region of the outer root sheath adjacent to the insertion of the arrector pili muscle, known as the bulge region. The percentage of double immunofluorescence cytochemically stained β1 integrin-and BrdU-positive cells was 81% in all β1-positive cells, and 94% in all BdrU-positive cells. The percentage of P63-and BrdU-positive cells was 65% in all P63-positive cells, and 86% in all BdrU-positive cells. The percentage of CD34- and BrdU-positive cells was 30% in all CD34-positive cells, and 62% in all BdrU-positive cells. Conclusion    Among these molecular markers, β1 integrin, with highest accordance with BrdU, is the best molecular marker for ESCs, and P63 is the next best. Related Articles | Metrics

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Biodistribution and tumor targeting of 131I-rMIF in tumor-bearing mice JIANG Shi-qin, ZHANG Chao, LIANG Ting, SONG Jing, SUN Hu-kui, HOU Gui-hua JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2012, 50 (1): 81-.   Abstract （7661）      PDF（pc） （3469KB）（309）       Save Objective   To evaluate biodistribution and tumor targeting of 131I-labeled recombinant macrophage migration inhibition factor(rMIF) in tumor-bearing mice, and to provide an imaging marker for early diagnosis of hepatocellular carcinoma. Methods   MIF was labeled with 131I using Iodogen method and the labeled rate and stability were identified with paper chromatography. The specific binding with hepatocellular carcinoma H22cells in vitro was analyzed by cells uptake assay. Biodistribution and tumor targeting were analyzed after injection of 131I-rMIF through the tail vein in tumorbearing mice. Results   131I-rMIF, with a good bioactivity, was successfully prepared. The labeled rate of 131I-rMIF was 87.34%, radiochemical purity was 94.95%, and the stability was good. The uptake of 131I-rMIF by tumor cells was much higher than that of the controls (free Na131I) in 0.5, 1, 2 and 4h (P<0.05). 131I-rMIF was mainly metabolized through the liver and kidney. And higher radioactivity was detected within tumors (target). T/NT (target-to-non-target) ratios were 2.701±0.230, 3.931±0.281, 4.242±0.111 and 3.587±0.241 at 1, 3, 6 and 24h after injection, separately(P<0.05). The result of autoradiography showed that 131I-rMIF could be specifically localized in tumors after 6h. Conclusion   131I-rMIF, with good stability, good labeled ratios and rapid targeted distribution in tumor cells, may be used for detection of hepatocellular carcinoma. Related Articles | Metrics

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Expression and significance of RhoE／Rnd3 protein in adenomyosis LIU Jing, ZHANG Hui, YAN Lei, ZHAO Xing-bo, LI Ming-jiang JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2012, 50 (5): 116-.   Abstract （6461）      PDF（pc） （1685KB）（339）       Save Objective   To investigate expression of RhoE／Rnd3 protein in human adenomyosis and its potential clinical effect． Methods   The immunohistochemical method was employed to detect RhoE／Rnd3 protein expression of the eutopic endometrium obtained from 32 patients without adenomyosis(the control group), and the eutopic(A group) and ectopic (B group)endometrial tissues surgically obtained from 38 patients with adenomyosis(the experimental group). Expression was detected in endometrial glandular cells, stromal cells and smooth muscle cells of the normal and the adenomyosis groups． Results   ① In endometrial glandular cells： expressions of RhoE／Rnd3 in the control group were significantly higher than those of the eutopic(t=11.3, P<0.05) and the ectopic (t=9.33，P<0.05) endometria of the experimental groups, but there was no significant difference between the latter two groups(t=1.63， P>0.05). ②In stromal and smooth muscle cells there was no significant variance of RhoE /Rnd3 in the stromal cells of the three groups, and there was no significant difference in the smooth muscle cells between the control group and the experimental group (P>0．05).  ③ Expression of RhoE／Rnd3 in endometrial glandular cells was related to the course of adenomyosis. Expression levels in tissues from patients whose clinical courses exceeded two years were obviously lower than those from patients whose clinical courses were less than two years(P＜0.05). Conclusion   The RhoE／Rnd3 protein may play a considerable role in the development of adenomyosis, and it could be an important tumorsuppressing gene. Related Articles | Metrics

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Differential diagnosis of Graves′ disease and Hashimoto′s thyroiditis by different types peak systolic velocity of the superior thyroid artery XU Ping, YU Guo-Fang, LI Xia JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2009, 47 (6): 62-64.   Abstract （4705）      PDF（pc） （379KB）（181）       Save Objective To explore the values of peak systolic velocity (PSV) （PSV left, PSV right, PSV max, PSV min, and PSV mean）of the superior thyroid artery in differential diagnosis of Graves′ disease (GD) and Hashimoto thyroiditis (HT). Methods 19 patients with GD, 18 patients with HT and 20 normal people were subjected to 2D and color Doppler ultrasound examination to obtain PSV of bilateral superior thyroid arteries and conclude the mean PSV of the two sides. Sensitivities and specificities of different types PSV in differential diagnosis of GD and HT were analyzed using ROC curves. Results There were statistic differences between any two groups in the value of PSVleft, PSVright,  PSVmax, PSVmin and PSVmean, but only the area under the curve of PSVmean and PSVmax was more than 0.9. Their cut-off points were 71cm/s and 85cm/s, and sensitivities and specificities were as follows: PSVmean：94.7%, 83.3%; PSVmax：89.5%, 88.9%. Conclusions PSVmax and PSVmean have high diagnostic accuracies. We can only measure one side PSV value when the first side is over 85cm/s and must measure both when the first side is below 85cm/s. Related Articles | Metrics

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Construction of a hTRX-PR39 recombinant adeno-associated virus vector RUAN Xi-yun1, WEI Min2, BI Jian-zhong3, YANG Guang-xiao4, WANG Quan-ying4 JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2011, 49 (7): 61-64.   Abstract （4617）      PDF（pc） （701KB）（245）       Save Objective     To construct a vector of recombinant adeno-associated virus(AAV) containing the fusion gene hTRX-PR39, and explore the role of fusion gene hTRX-PR39 as a target gene in the treatment of cerebral ischemic disease. Methods       The constructed fusion gene hTRXPR39 was inserted into the EcoRIBamHI site of vector plasmid pSSCMV, and the vector of hTRX-PR39 recombinant AAV was constructed. The recombinant AAV stock was packaged. Renal embryo 293 cells were co-transfected with the recombinant AAV vector of plasmid pSSCMV/hTRX-PR39,packaging plasmid pAAV/Ad and helper adenovirus plasmid pFG140. Recombinant AAV was produced by homologous recombination of the 3 plasmids in renal embryo 293 cells and its titer was measured by quantitative dot blot hybridization. Results      The vector of hTRXPR39 recombinant AAV was successfully constructed. High titer of recombinant AAV was obtained by homologous recombination in renal embryo 293 cells(3.46×1012-3.46×1013PFU/mL). Conclusion        The recombinant AAV vector encoding the gene hTRX-PR39 was successfully constructed in this study by molecular cloning and in vitro recombination techniques, laying the foundation for further research into genetic therapy of cerebral ischemic disease. Related Articles | Metrics

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Application of geographic information system in the control of COVID-19 epidemic Xiujun LI,Xinlou LI,Kun LIU,Xiaobo ZHAO,Meng MA,Bo SUN Journal of Shandong University (Health Sciences)    2020, 58 (10): 13-19.   DOI: 10.6040/j.issn.1671-7554.0.2020.0891 Abstract （4294）   HTML （0）    PDF（pc） （1293KB）（98）       Save Since the outbreak of coronavirus disease 2019 (COVID-19) epidemic, the geographic information system (GIS) has played an important role in explaining the epidemic distribution, characteristics of regional transmission, risk assessment, and early prediction and warning, which greatly helped the disease control and prevention. In this study, the application of GIS in COVID-19 prevention and control was reviewed, hoping to provide reference for future improvement in the prevention and control measures. Reference | Related Articles | Metrics

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A general overview of neuromodulation Di ZHANG,Meng YU,Xia LIU Journal of Shandong University (Health Sciences)    2020, 1 (8): 50-60.   DOI: 10.6040/j.issn.1671-7554.0.2020.0602 Abstract （4152）   HTML （164）    PDF（pc） （4269KB）（1436）       Save Neuromodulation refers to a series of biomedical engineering techniques which normalize or modulate the function of the nervous system through invasive or non-invasive delivery of physical or chemical stimulus. In the past thirty years, with the in-depth understanding of the nervous system and its mechanisms of action, as well as the rapidly developing modern techniques, neuromodulation has evolved from basic concept to clinical applications. At the same time, new concepts and alternative methods of neuromodulation are constantly being innovated, tested and put into practice. The advancing of neuromodulation techniques will not only provide new tools for basic neuroscience research but also offer novel therapeutic options for neurological and psychiatric disorders. In this overview, we will briefly introduce the current status of different types of neuromodulation techniques, including the clinical applications, underlying mechanisms of their therapeutic effects and advantages/disadvantages, as well as the prospects for the future. Table and Figures | Reference | Related Articles | Metrics

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Role of ET-1receptors in pulmonary hypertension due to left to right shunt WANG Li-juan,ZHAO Cui-fen,XIA Wei JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    Abstract （3991）      PDF（pc） （311KB）（630）       Save Objective: To investigate the mechanism of pulmonary hypertension, the effect of ET-1 receptors in pulmonary hypertension due to left to right shunt was studied. Methods: Thirtyfour 150-200g male Wistar rats were randomly selected and divided into three groups: experiment groupⅠ(n=15), experiment groupⅡ (n=9) and control group (n=10). A cervical left to right shunt pulmonary hypertension model was established by the cuff technique in the experiment groups. After four weeks, the rats of experiment group Ⅱ underwent a 12-week intravenous administration of BQ123 ［cyclopropane (Trp-Asp-Pro-Val-Leu)］. The systolic pulmonary artery pressures (sPAPs), ET-1 concentrations and the expressions of the prepro ET-1(PPET-1) mRNA, ETA receptor mRNA and ETB receptor mRNA of pulmonary tissues in the three groups were measured. Results: The sPAP and ET-1 concentrations were significantly higher in the experiment groups than in the control group (P＜0.01), and they were higher in experiment groupⅠthan in experiment groupⅡ. The mRNA expression of ET-1 and ETA receptors were distinctly elevated in the experiment groups (P＜0.01), and they were higher in experiment groupⅠthan in experiment groupⅡ(P＜0.05). ETB receptors changed inversely. Conclusions: The above results indicate that different ET receptors may exhibit different effects on pulmonary hypertension progression due to left to right shunt. Related Articles | Metrics

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Clinical value of serum CYFRA 21-1, NSE and CEA in the  diagnosis and prognosis of lung cancer SHANG Guan-Hong1，XIAO Wei1, DONG Liang1, LI Yu1, TIAN Hui2, ZHENG Tian-ying3 JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2010, 48 (12): 134-137.   Abstract （3943）      PDF（pc） （532KB）（382）       Save Objective    To investigate the clinical significance of serum cytokeratin19 fragments (CYFRA 21-1), neuron specific enolase ( NSE) and carcinoembryonic antigen (CEA) in the diagnosis and prognosis of lung cancer． Methods     Serum CYFRA21-1, NSE and CEA were measured with chromatometrychemoluminescence method in 68 patients with lung cancer at different TNM stages(the lung cancer group)， 36 patients with benign lung diseases( the lung benign group)， and 28 healthy people(the healthy group), and the relationship of CYFRA21-1, NSE and CEA with TNM stages were analyzed．  Survival curves were estimated to analyze the relationship of CYFRA21-1, NSE and CEA with survival time using Kaplan-Meier analysis. Results    Serum levels of CYFRA 211, NSE and CEA were significantly higher in the lung cancer group than in lung benign and healthy groups(both P<0.01). There were positive relations of CYFRA 21-1, NSE and CEA with the TNM stage, and negative relations with survival time（P<0.01）. CYFRA21-1 had the highest sensitivity(78.72%) in non-small cell lung cancer, and NSE had the highest sensitivity(91.23%)in small cell lung cancer. Conclusion     Serum levels of CYFRA 21-1, NSE and CEA are significantly raised in patients with lung cancer， which suggests that they are important markers for the early diagnosis, development and prognosis of lung cancer． Related Articles | Metrics

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Comparison of clinical efficacy of Qingfei Paidu decoction combined with western medicine in 43 cases and single western medicine in 46 cases in the treatment of COVID-19 YU Xueyuan, ZHANG Shuo, YAN Fangfang, SU Dezhen Journal of Shandong University (Health Sciences)    2020, 58 (12): 47-53.   DOI: 10.6040/j.issn.1671-7554.0.2020.0870 Abstract （3824）      PDF（pc） （2409KB）（499）       Save Objective To retrospectively evaluate the clinical efficacy of Qingfei Paidu decoction in the treatment of coronavirus disease 2019(COVID-19)and to explore the possible mechanism. Methods A total of 89 COVID-19 patiests whose date of discharge ranged from Feb. 10 to Apr. 1, 2020 were selected in the infection ward of the East Hospital of Renmin Hospital of Wuhan University. According to whether Qingfei Paidu decoction was used, 89 cases were divided into basic treatment group(n=46)and combined treatment group(n=43). The paitents in basic treatment group were only given western medicine treatment, while those in combined treatment group were treated with modified Qingfei Paidu decoction on the basis of western medicine treatment plan. The changes of blood routine, C reactive protein(CRP), interleukin-6(IL-6), cellular immunity, biochemical series and coagulation series were compared between the two groups before and after treatment. The changes of lung CT, the time of nucleic acid turning negative, and the patients hospitalization time were collected. A pairwise or non-paired t test was used to compare numerical data with normal distribution; the counting data were described by relative numbers, and the differences between groups were tested by chi-square test. Results (1) After the treatment, the expression levels of neutrophil ratio(NEU%), CRP, IL-6, creatine kinase(CK), lactate dehydrogenase(LDH), fibrinogen(Fib)and fibrinogen degradation product(FDP)were decreased in the two groups(P<0.05); the counts of lymphocyte ratio(LYM%), CD3, CD4 and CD8 T cells were significantly increased in the two groups(P<0.05); the levels of serum creatinine(SCr)and D-dimer(DD)were decreased while the estimated glomerular filtration rate(eGFR)was increased in the combined treatment group(P<0.05). (2) Compared with the basic treatment group, the difference of CD3 and IL-6 before and after treatment in the combination treatment group was statistically significant(P<0.05). There was no difference in the degree of improvement of chest CT between the two groups. The length of hospital stay in the combined treatment group was shorter than that in the basic treatment group(P<0.01), and the nucleic acid conversion time was shorter without statistical difference. Conclusion (1) The level of inflammation is reduced, cellular immunity is enhanced, renal function is improved, and hypercoagulability is reduced in both the basic treatment group and combined treatment group. (2) Compared with the basic treatment group, modified Qingfei Paidu decoction can significantly reduce the level of IL-6 and increase the level of CD3 of COVID-19 patients. The treatment of integrated traditional Chinese medicine and western medicine can significantly inhibit the inflammatory reaction, enhance the bodys immunity, and shorten the length of hospitalization and nucleic acid negative time, which help to get a better clinical efficacy. Reference | Related Articles | Metrics

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JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2008, 46 (12): 1207-1208.   Abstract （3620）      PDF（pc） （205KB）（214）       Save Related Articles | Metrics

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Advances on the etiology and pathogenesis of osteoporosis LUO Xianghang, ZHOU Ruoyu Journal of Shandong University (Health Sciences)    2021, 59 (6): 10-14.   DOI: 10.6040/j.issn.1671-7554.0.2021.0324 Abstract （3315）   HTML （233）    PDF（pc） （762KB）（944）       Save Osteoporosis is the most common metabolic bone disease. The incidence of osteoporosis as well as osteoporotic fracture increases gradually with age, seriously endangering patients health. The core pathogenesis of osteoporosis is that various factors act on some stages of bone remodeling, resulting in stronger bone resorption than bone formation. These factors include genetic factors, age, endocrine and lifestyle. It is of great significance to study the pathogenesis and etiology of osteoporosis for its prevention and treatment. Reference | Related Articles | Metrics

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A new method to identify parathyroid glands in surgery: parathyroid autofluorescence imaging(a report of 6 cases) ZHUANG Dayong, HE Qingqing, ZHOU Peng, YUE Tao, LI Xiaolei, XU Jing Journal of Shandong University (Health Sciences)    2020, 58 (11): 98-102.   DOI: 10.6040/j.issn.1671-7554.0.2020.1168 Abstract （3238）      PDF（pc） （3599KB）（794）       Save Objective To introduce a new method to identify and protect the parathyroid glands in thyroid surgery with autofluorescence imaging. Methods A total of 6 patients who underwent thyroid surgery at our department in June 2020 were enrolled. The preoperative parathyroid hormone, serum calcium and phosphorus measurements were within the normal range. For the 3 patients with nodular goiter, 1 received thyroid lobectomy, and 2 received total thyroidectomy. Three patients with papillary thyroid carcinoma underwent total thyroidectomy, 2 of whom received simultaneous central lymph nodes dissection, and 1 received simultaneous lymph nodes dissection of central area + unilateral neck. Fluorescence surgical navigation system was used to perform autofluorescence imaging of parathyroid glands at the following time points: after the thyroid glands were exposed, after the parathyroid glands were exposed, after thyroidectomy, and after the completion of central lymph node dissection. Results Twenty-two parathyroid glands in 6 patients were not detected with autofluorescence before being exposed, but could be detected with obvious autofluorescence after being exposed. The autofluorescence imaging rate was 100%. The thyroids, lymph nodes and adipose tissues did not show substantial autofluorescence. One parathyroid gland still showed autofluorescence after being isolated. Conclusion Parathyroid glands show autofluorescence characteristics in the near-infrared spectrum. This can help to identify the parathyroid glands during thyroidectomy, but cannot be used as a navigation tool for parathyroid recognition. Reference | Related Articles | Metrics

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SARS-CoV-2 infection, innate immunity and inflammatory response Xi ZHOU,Muhan HUANG,Yujie REN,Yang QIU Journal of Shandong University (Health Sciences)    2021, 59 (5): 15-21.   DOI: 10.6040/j.issn.1671-7554.0.2021.0368 Abstract （3191）   HTML （188）    PDF（pc） （1512KB）（955）       Save Coronavirus disease 2019 (COVID-19), which is caused by a novel coronavirus named severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), has become the worst pandemic and public health crisis across the globe once a century. This pandemic has caused huge losses in both human lives and global economy. Innate immunity is the first line of defense against pathogenic invasions. Extensive studies by scientists in China and the world have reported that SARS-CoV-2 can employ multiple strategies to evade host innate immunity, and such immune evasion mechanisms have become critical contributing factors for the pathogenicity of SARS-CoV-2. On the other hand, the pathogenesis of COVID-19 has been found to be closely relevant with the pro-inflammatory responses induced by SARS-CoV-2 infection in humans. This paper provides a brief review to the relationship between SARS-CoV-2 infection and innate immunity as well as inflammation. Reference | Related Articles | Metrics

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Current status and prospect of viral vaccines research and development Kai WANG Journal of Shandong University (Health Sciences)    2021, 59 (5): 8-14.   DOI: 10.6040/j.issn.1671-7554.0.2021.0273 Abstract （3017）   HTML （174）    PDF（pc） （1285KB）（1237）       Save Virus infection can cause several diseases in human. In the past decades, outbreaks and epidemics of respiratory infectious diseases caused by coronary viruses, especially coronavirus disease 2019 (COVID-19), have become serious health hazards and posed huge challenges to public health. The development and application of viral vaccines have been an important means to counteract the spread of viruses. Historically, poliovirus and hepatitis B virus vaccines have successfully suppressed the transmission of diseases and provided a model for the development of vaccines. Recently, the emergence of novel technologies and platforms such as recombinant viral vector and mRNA vaccine has provided new approaches to the development of vaccines against emerging infectious diseases including COVID-19. This paper reviews the current situation and progress of platforms, summarizes problems to be solved, and forecasts the directions of viral vaccines development. Reference | Related Articles | Metrics

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Frontier research hotspot and progress in assisted reproductive technology SHI Yuhua, WANG Qiumin, QI Dan Journal of Shandong University (Health Sciences)    2021, 59 (9): 97-102.   DOI: 10.6040/j.issn.1671-7554.0.2021.0826 Abstract （2903）   HTML （107）    PDF（pc） （1425KB）（1491）       Save Assisted reproductive technology(ART), as an emerging discipline, has developed rapidly in recent decades, and has continuously made breakthroughs in the field of human reproductive health. The development and application of new technologies should be based on laws and ethics, with safety as the prerequisite, and continuously improve the effectiveness of the technology. In recent years, in order to improve the level of reproductive health and quality of newborn population, preimplantation genetic testing(PGT), mitochondrial replacement(MRT), freeze-thaw embryo transfer(FET)and cell therapy have become the focus of reproductive experts. This paper analyzes the research progress on hot issues in the field of ART. Reference | Related Articles | Metrics

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Contents and ratios of type I and type III collagens in normal skin and hypertrophic scar in people of different ages WANG Cheng, RONG Yanhua, SHEN Yuming, ZHANG Guoan JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2016, 54 (11): 64-67.   DOI: 10.6040/j.issn.1671-7554.0.2015.1043 Abstract （2858）      PDF（pc） （1915KB）（1287）       Save Objective To quantify the contents of type I and III collagens and their ratios in normal skin and hypertrophic scar in people of different ages, and to explore the rule and cause of changes. Methods Normal human skin specimens and hypertrophic scar specimens were obtained from spontaneously aborted fetus and people of different ages, including the children and adolescent group(≤18 years), adult group(19-50 years), elderly group(>50 years). The total collagen contents were determined by hydroxyproline method. The ratios of types I and III collagens were examined by immunohistochemistry, and their contents were calculated. Results The difference of the contents of types I and III collagens and their ratios in normal skin were statistically significant among different age groups(P=0.01). Their ratios increased with the increase of age, and the differences were statistically significant among different age groups(P=0.02). The difference of contents of types I and III collagens and their ratios in hypertrophic scars were statistically significant among all groups, also between any two groups(P=0.03). In every age group, the difference of the contents of types I and III collagens and their ratios between normal skin and hypertrophic scar was statistically significant (P=0.02). Conclusion With age increasing, the changes of the ratios of types I and III collagens in the normal skin changes, which results in changes of the tension and elasticity of skin and the healing process after injury. The contents of types I and III collagens and their ratios significantly changes in hypertrophic scars, resulting in different structure of scar from normal skin. Reference | Related Articles | Metrics

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Relation between CD3+CD16+CD56+ NKT cells and immune  status in patients after renal transplantation XIAN Wan-hua1， TIAN Jun1， GUAN Guang-ju2， BI Zheng3， CUI Xian-quan1， SUN Huai-bin1，LI En-gang1， PENG Zhi-guo1， GENG Li-na1 JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)    2010, 48 (11): 54-57.   Abstract （2824）      PDF（pc） （375KB）（429）       Save Objective    To explore the relation of expression of peripheral blood CD3+CD16+CD56+ natural killer T（NKT） cells with immune status and different concentrations of calcineurin inhibitor (CNI) in patients after renal transplantation. Methods    92 patients after renal  transplantation were divided into three groups according to rejection and  concentrations of CNI：patients without rejection in normal concentration(group A，58 cases), patients without rejection in lower concentration (group B，8 cases), and patients with rejection (group C，26 cases). 10 healthy individuals were selected as the controls. CD3+，CD3+CD4+，CD3+CD8+，CD3-CD16+CD56+NK and CD3+CD16+CD56+ NKT cells and human leucocyte antigen(HLA) antibodies were analyzed by flow cytometry.  Results    Percentages of CD3+CD16+CD56+ NKT cells in groups A,B,C and D were (4.29±2.57)%,(4.31±2.08)%,(1.23±1.06)% and (3.98±2.26)%，respectively. Percentages of NKT and NK cells，CD4+/CD8+，and the HLA antibodypositive rate in groups A and B had no significant difference compared with those in group D. The percentage of CD3+CD16+CD56+NKT  cells was lower in group C compared with that in group D (P<0.05). Percentages of NK cells and CD4+/CD8+, and the HLA antibodypositive rate in group C were significantly higher than in group D(P<0.05).   Conclusion     CD3+CD16+CD56+ NKT cells are lowly expressed when rejection occurs，and HLA antibodies occur in these patients. Expression of NKT cells is normal and stable in patients without rejection, and is not affected by CNI concentrations. NKT cells play an important role in immunosuppression in patients after renal transplantation. Related Articles | Metrics