Abstract: To investigate the expression of CyclinE in human colon carcinoma and its relation with the pathogenesis of colon carcinoma. Methods: Insitu hybridization and immunohistochemistry were used to detect the expressions of CyclinE mRNA and CyclinE protein in 40 cases of colon carcinoma in various stages, and 10 colon adenoma, and 10 normal colon mucosa. The results were analyzed combined with clinic and pathology materials. Results: No CyclinE mRNA and CyclinE protein were found in normal colon mucosa, and 62.5% and 57.5% respectively were found in colon carcinoma, which were significantly higher than the other two groups (P＜0.05). CyclinE mRNA had close relation with infiltration depth and lymphmetastasis (P＜0.05), but it had no relation with extent of histology differentiation and liver metastasis (P＞0.05). Expression of CyclinE protein was consistent with expression of CyclinE mRNA, but positive expression of CyclinE protein in liver metastasis group was higher than in nonlive metastasis group (P＜0.05). Conclusion: Expressions of CyclinE mRNA and CyclinE protein in colon carcinoma increase significantly and have close correlation with biological behavior of colon carcinoma, suggesting high expression of CyclinE has relation with pathogenesis of colon carcinoma and CyclinE may be a new assistant index for early diagnosis and prognosis for colon

Key words: Colon carcinoma, CyclinE, Insitu hybridization, Immunohistochemistry