Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (10): 33-41.doi: 10.6040/j.issn.1671-7554.0.2022.0169

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Proteomic analysis of serum exosomes in pancreatic cancer with different stages of progress

LI Yanru, LI Juan, LI Peilong, DU Lutao, WANG Chuanxin   

  1. Department of Clinical Laboratory, The Second Hospital of Shandong University, Jinan 250033, Shandong, China
  • Published:2022-09-30

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Abstract: Objective To screen the differential proteins of serum exosomes in healthy controls and pancreatic cancer patients with different degrees of progression, draw the profile, and analyze their functions and biological processes. Methods Exosomes were quantitatively analyzed with label-free quantitative proteomic analysis technology. The differential proteins were screened and bioinformatic analysis was performed. The proteins associated with pancreatic cancer progression were serologically verified with Western blotting. Results A total of 2 242 proteins were detected in the control, non-metastatic and metastatic pancreatic cancer samples with label-free quantitative proteomic analysis and a total of 664 proteins with statistically significant differences were identified, which were mainly involved in vesicle-mediated transport, focal adhesion and extracellular cortex matrix(ECM)receptor interaction. Moreover, there were 5 proteins with statistically significant differences among the three groups, of which leucine-rich alpha-2-glycoprotein 1(LRG1)was highly expressed in serum exosomes of metastatic pancreatic cancer. Conclusion There are differences in protein expression patterns among healthy controls, non-metastatic and metastatic pancreatic cancer patients, and the differential proteins are mainly enriched in focal adhesion and ECM-receptor interaction and other signaling pathways. LRG1, which is different in all three groups, may be a serological marker for monitoring the progression of pancreatic cancer.

Key words: Pancreatic cancer, Exosome, Proteomics, Bioinformatics, Leucine-rich alpha-2-glycoprotein 1

CLC Number: 

  • R735.9
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