Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (4): 91-96.doi: 10.6040/j.issn.1671-7554.0.2019.028

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Diagnostic value of serum exosomal miR-20b-5p for non-small cell lung cancer

WEI Ping1, DU Lutao1, 2, WANG Qing1, ZHAN Yao1, XIE Yujiao1, ZHANG Shujun1, DUAN Weili1, WANG Chuanxin1, 2   

  1. 1. Department of Clinical Laboratory, The Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    2. Tumor Marker Detection Engineering Laboratory of Shandong Province, Jinan 250033, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To explore the diagnostic value of serum exosomal miR-20b-5p for non-small cell lung cancer(NSCLC). Methods Serum samples of 144 NSCLC patients(NSCLC group)and 138 healthy controls(healthy group)were collected. Exosomes in the serum were extracted and characterized, and miRNA was abstracted. The expression of exosomal miR-20b-5p in the NSCLC group and healthy control group were compared with RT-qPCR. The diagnostic value of exosomal miR-20b-5p was evaluated with receiver operating characteristic(ROC)curve. Results Purified exosomes had typical features. The expression of miR-20b-5p was significantly higher in the NSCLC group than in the healthy control group (P<0.001). The area under the ROC curve(AUC)was 0.849(95%CI:0.802~0.889). The diagnostic sensitivity was 73.61%, and the specificity was 70.29%. Conclusion Serum exosomal miR-20b-5p is highly expressed in NSCLC with highly clinical diagnostic value. It can serve as a new biomarker to assist the diagnosis of NSCLC.

Key words: Serum exosomal, MiR-20b-5p, Non-small cell lung cancer, Diagnose

CLC Number: 

  • R734.2
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