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10 April 2019
Volume 57 Issue 4

Roles and mechanisms of TCONS_00016478 in atrial energy metabolic remodeling by regulating PGC-1α/ PPARγ pathway in rabbit models of atrial fibrillation

LI Jianhua, LI Zhan, JIA Xiaomeng, DU Juanjuan, MA Shenzhou, LIU Donglu, ZHANG Yong, ZHANG Yujiao, HOU Yinglong

Journal of Shandong University (Health Sciences). 2019, 57(4):  1-8.  doi:10.6040/j.issn.1671-7554.0.2018.706

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Objective To investigate the roles and mechanisms of TCONS_00016478 in atrial energy metabolic remodeling by regulating peroxisome proliferator-activated receptor gamma coactivator 1-alpha(PGC-1α)/ peroxisome proliferator-activated receptor gamma(PPARγ)pathway in rabbit models of atrial fibrillation(AF). Methods The long non-coding RNA(lncRNA)expression profiles of right atria were investigated in AF and non-AF rabbit models using RNA sequencing technique. A total of 18 male and female adult New Zealand white rabbits weighting 2.0~2.5 kg were randomly divided into 3 groups: sham group(n=6, received thoracotomy only), negative control group(n=6, right atria infected with negative control lentiviruses), and lenti-RNAi-TCONS_00016478 group(n=6, right atria infected with TCONS_00016478 silencing lentiviruses). The atrial effective refractory period(AERP)and AF inducibility were measured before infection and 7 d after infection. After 7 d infection, the rabbits were sacrificed and atrial samples were collected. The gene and protein expressions were detected with qRT-PCR and Western blotting, respectively. The accumulation of glycogens and lipid droplets in atrial myocytes were assessed with periodic acid-Schiff(PAS)and Oil Red O staining, respectively. Results After 7 d infection, the AERP was significantly shortened in lenti-RNAi-TCONS_00016478 group(80.667±1.453 vs 71.750±2.411, t=3.168, P=0.034); however, no significant difference was found in the sham group(80.083±1.044 vs 79.333±0.333, t=0.684, P=0.531)and negative control group(81.083±2.599 vs 80.000±2.646, t=0.022, P=0.983). AF was induced in 3 rabbits in lenti-RNAi-TCONS_00016478 group, while no AF was induced in the sham and negative control groups. There were significant differences in the gene and protein expressions of TCONS_00016478(F=126.042, P<0.001), PGC1-α(F=43.998, P<0.001), PPARγ(F=417.863, P<0.001), glucose transporter type 4(GLUT4)(F=98.043, P<0.001) and carnitine palmitoyltransferase 1(CPT1)(F=105.096, P<0.001) among the 3 groups. Compared with the sham group, the lenti-RNAi-TCONS_00016478 group had lower expressions of TCONS_00016478, PGC1-α, PPARγ, GLUT4, and CPT1(all P<0.001), Bioinformatic analysis showed lncRNA TCONS_00016478 and PGC-1α were closely related to myocardial energy metabolism. But significantly increased accumulation of glycogens and lipid droplets in atrial myocytes. Conclusion TCONS_00016478 modulates atrial energy metabolic remodeling during AF through PGC-1α/PPARγ pathway.

Effects of c-Jun N-terminal kinase regulated by CD40siRNA on the autophagy of cardiomyocytes in rats with autoimmune myocarditis

SUN Honglin, HAN Bo, WANG Jing, GAO Ling, ZHU Mei, JIANG Diandong, LÜ Jianli

Journal of Shandong University (Health Sciences). 2019, 57(4):  9-14.  doi:10.6040/j.issn.1671-7554.0.2018.1344

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Objective To investigate the effects of CD40siRNA regulated c-Jun N-terminal kinase(JNK)pathway on the autophagy of cardiomyocytes in experimental autoimmune myocarditis(EAM). Methods A total of 32 healthy male Lewis rats aged 6 to 8 weeks were randomly divided into the EAM group, EAM+CD40siRNA group, EAM+siRNA control group, and normal control group(NC group), with 8 rats in each group. The EAM models were established by injecting mixed porcine heart immunoglobulin(0.2 mL/rat)into the hind paw pad of rats in the first 3 groups on day 0 and day 7. Rats in the NC group were injected with 0.2 mL/rat of phosphate buffered saline(PBS). On day 7, rats in the EAM+CD40siRNA group were injected with 25 μL of CD40siRNA lentiviral vector in the tail vein, and rats in the EAM+siRNA control group were injected with 25 μL of LsiRNA lentiviral vector. The body weight of rats was measured every 3 days. All rats were sacrificed on day 21. Pathological changes of the myocardial tissues were observed 山 东 大 学 学 报 (医 学 版)57卷4期 -孙红林,等.CD40siRNA调控c-Jun氨基末端激酶对自身免疫性心肌炎大鼠心肌细胞自噬的影响 \=-with a light microscopy and pathological scores were calculated. The JNK was immunohistochemically stained. The autophagy-related protein Beclin1 and microtubule-associated protein light chain 3-Ⅱ/Ⅰ ratio(LC3-Ⅱ/Ⅰ)were detected with Western blotting. Results During the experiment, no rats died. The body mass of rats in the NC group increased faster than that in the EAM group(P<0.05). The EAM+CD40siRNA group had significantly lower pathological scores than the EAM group(P<0.001). The EAM+CD40siRNA group and NC group had significantly lower LC3-Ⅱ/Ⅰ and Beclin1 than the EAM group, and there was a positive correlation between JNK and LC3-Ⅱ(r=0.985, P<0.001). Conclusion The CD40siRNA can relieve the inflammation of EAM rats, possibly by downregulating the JNK pathway and inhibiting the autophagy of cardiomyocytes.

Natural killer cells regulate Th17/Treg cell immune response through modulating the function of dendritic cells during chlamydial lung infection

ZHOU Xiaoqing, LI Jing, ZHAO Lei, TANG Wei, YANG Xi, WANG Hong

Journal of Shandong University (Health Sciences). 2019, 57(4):  15-19.  doi:10.6040/j.issn.1671-7554.0.2019.032

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Objective To explore the mechanism of natural killer(NK)cells in regulating of Th17/Treg cell responses during chlamydia muridarum(Cm)lung infection. Methods A pulmonary infection model of Cm was established. The spleen dendritic cells(DCs)from Cm-infected mice with or without NK cells depletion were obtained by application of CD11c⁺ MicroBeads. The naïve mice were given intravenous injection of DCs from different groups of mice, and divided into NK^-DCs recipients group(n=13)and NK⁺DCs recipients group(n=13). After adoptive transfer, the same infection method was used to recipient mice with Cm. The degree of disease was compared between the two groups. Flow cytometry was used to detect the changes of Th17 and Treg cells in the spleen and lung of mice. Results Compared with NK⁺DCs recipients, NK^-DCs recipients exhibited aggravated disease, higher weight loss and severe lung injury. Immunological analysis showed the proportion of Th17 cells in spleen and lung was reduced while the proportion of Treg cell response was enhanced, which contributed to imbalance of Th17/Treg response in NK^-DCs recipients. 山 东 大 学 学 报 (医 学 版)57卷4期 -周小青,等.自然杀伤细胞通过树突状细胞调控衣原体肺部感染中Th17/Treg免疫应答平衡 \=- Conclusion NK cells can effectively initiate Th17 cells response and inhibit Treg cell response by modulating DCs and thus play an important role in controlling chlamydial infection.

Effects of sleeve gastrectomy on lung injury in type 2 diabetic rats

DU Hao, CHENG Yugang, HUANG Xin, LIU Shaozhuang, ZHANG Guangyong, HU Sanyuan

Journal of Shandong University (Health Sciences). 2019, 57(4):  20-26.  doi:10.6040/j.issn.1671-7554.0.2019.081

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Objective To investigate the effects of sleeve gastrectomy on lung injury in type 2 diabetic rats and to explore the mechanism. Methods Type 2 diabetic models were induced by intraperitoneally injecting streptozotocin(STZ)to Wistar rats. The diabetic rats were randomly assigned to the sleeve gastrectomy group(SG group, n=10)and Sham group(n=10). The body weight, food intake, fasting blood glucose(FBG)and glucose tolerance were measured postoperatively. In postoperative week 16, the rats were sacrificed. The lung tissues were collected and the histomorphology was observed with HE staining. The expressions of inflammation factors interleukin-6(IL-6), tumor necrosis factor α(TNF-α)and oxidative stress indicator nitrotyrosine(NT)were determined with Western blotting and immunohistochemistry. The expressions of nitric oxide(NO)and malondialdehyde(MDA), as well as the activity of superoxide dismutase(SOD)were determined with biochemical testing. Results Only 1 rat in the SG group died after surgery. Compared with the sham group, SG group had better glucose control(FBG: F=100.70, P<0.01, AUC_OGTT: F=88.15, P<0.01), mitigated thickness of interalveolar septum, reduced hemorrhage, lower expressions of IL-6, TNF-α, NT, NO and MDA(P<0.01), but higher activity of SOD(P<0.01). Conclusion Sleeve gastrectomy can relieve the lung injury in type 2 diabetic rats, possibly by inhibiting the expressions of inflammation factors and improving oxidative stress in lungs.

Cloning and functional characterization of two O-methyltransferases from Adiantum capillus-veneris L.

NI Rong, ZHANG Xiaoshuang, CHENG Aixia

Journal of Shandong University (Health Sciences). 2019, 57(4):  27-33.  doi:10.6040/j.issn.1671-7554.0.2019.027

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Objective To isolate and characterize the O-methyltransferase genes involved in the biosynthesis of flavonoids and phenypropanoids from Adiantum capillus-veneris L. Methods The full-length cDNA sequences of two O-methyltransferase genes were cloned by the RT-PCR method using the total RNA isolated from the Adiantum capillus-veneris L. as template, and designated as AcOMT1 and AcOMT2, respectively. The amplified ORFs were ligated into the pET-32a vector, and then were transformed into E.coli BL21 for expression. The recombinant proteins were purified using the Ni-NTA Sefinose His-bind column and used for enzyme assays. The protein homology models of AcOMT1 and AcOMT2 were generated by Swiss-model on-line server, and the key amino acids which were postulated to affect protein activity were predicted by PyMOL software. The analyses of phylogenetic tree and multiple sequence alignment were performed using MEGA v5.1 and DNAMAN 7.0.2 software, respectively. Results Two genes encoding putative O-methyltransferase were identified from the transcriptome sequencing database of Adiantum capillus-veneris L. and cloned from the plants. The sequence alignment and phylogenetic analysis indicated that AcOMTs belong to the family of type Ⅰ OMTs. Meanwhile, the enzymatic assays showed that AcOMT1 and AcOMT2 had the similar catalytic characteristics and broad substrate selectivety. Quercetin was the favorite substrate. Conclusion Two OMT genes were isolated and characterized from Adiantum capillus-veneris L., which would provide theoretical significance to elucidate the methylation modification of flavonoids in ferns.

Clinical observation of the effects of idebenone on Parkinsons disease

ZHANG Xiaotao, HE Tianqi, ZHU Meijia, TANG Jiyou, ZHAO Zhangning, MAO Fei, FANG Yuqing, LIU Xiaomin, MA Gaoting, ZHANG Xiaoyu, ZHANG Xiao, WANG Min, LI Xiuhua

Journal of Shandong University (Health Sciences). 2019, 57(4):  34-41.  doi:10.6040/j.issn.1671-7554.0.2018.1301

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Objective To investigate the effects of idebenone(IDE)on the motor and non-motor symptoms in patients with Parkinsons disease(PD)and to explore its neuroprotective effect. Methods A total of 200 PD patients were randomly divided into the IDE group(IDE+routine treatment)and routine group(routine treatment). The scores of Unified Parkinsons Disease Rating-III(on-stimulation), UPDRS-III(off-stimulation), Montreal Cognitive Assessment(MOCA), Pittsburgh Sleep Quality Index(PSQI), and Non-Motor Symptoms Scale for Parkinsons Disease(NMSS)were monitored before treatment and 3, 6, 9, 12, 15 and 18 months after treatment. Furthermore, changes of plasma superoxide dismutase(SOD)and uric acid were detected before treatment and 6, 12 and 18 months after treatment. Results (1) In month 3, 6, 9, 12, 15 and 18 after treatment, the score of UPDRS-III(on-stimulation)in the IDE group versus that in the routine group was 16.64±6.08 vs 16.79±6.44, 14.69±5.33 vs 15.64±6.16, 12.81±4.73 vs 14.58±5.86, 11.04±3.95 vs 13.54±5.56, 9.664±3.43 vs 12.41±5.11 and 8.471±3.09 vs 11.16±4.65, respectively. In month 3 and 6, there was no difference between the two groups (P>0.05). In month 9, 12, 15 and 18, the score was significantly lower in the IDE group than in the routine group(P<0.05). (2) In month 3, 6, 9, 12, 15 and 18 after treatment, the score of UPDRS-III(off-stimulation)in the IDE group versus that in the routine group was 23.09±8.63 vs 19.85±7.20, 21.10±7.13 vs 19.63±6.85, 19.30±6.24 vs 19.13±6.57, 17.39±5.37 vs 18.32±6.28, 15.65±4.78 vs 17.35±5.97 and 14.05±4.25 vs 16.10±5.64, respectively. In month 3, 6, 9 and 12, there was no difference between the two groups(P>0.05). In month 15 and 18, the score was significantly lower in the IDE group than in the routine group(P<0.05). (3) In month 3, 6, 9, 12, 15 and 18 after treatment, the MOCA score in the IDE group versus that in the routine group was 21.68±4.59 vs 22.13±4.64, 23.02±3.60 vs 22.69±4.23, 24.39±2.66 vs 23.40±3.69, 25.30±2.12 vs 24.20±3.18, 25.94±1.81 vs 25.17±2.66 and 24.72±2.23 vs 24.27±2.94, respectively. In month 3, 6 and 18, there was no significant difference between the two groups(P>0.05). In month 9, 12 and 15, the score was significantly higher in the IDE group than in the routine group(P<0.05). (4) In month 3, 6, 9, 12, 15 and 18 after treatment, the PSQI score in the IDE group versus that in the routine group was 6.36±4.26 vs 6.12±4.19, 5.33±3.84 vs 5.25±3.85, 4.59±3.45 vs 4.50±3.50, 3.32±2.92 vs 3.51±3.27, 2.71±2.38 vs 3.26±3.03 and 2.20±2.02 vs 3.04±2.84, respectively. In month 3, 6, 9, 12 and 15, there was no difference between the two groups(P>0.05). In month 18, the score was significantly lower in the IDE group than in the routine group(P<0.05). (5) In month 3, 6, 9, 12, 15 and 18 after treatment, the NMSS score in the IDE group versus that in the routine group was 54.6±31.87 vs 54.56±33.26, 52.01±30.11 vs 52.42±32.02, 46.84±30.76 vs 50.38±30.65, 41.20±29.60 vs 46.97±28.05, 38.77±26.67 vs 43.18±24.65, 36.63±25.39 vs 41.03±23.48, respectively. There was no significant difference between the two groups(P>0.05). (6) In month 6, 12 and 18 after treatment, the level of uric acid in the IDE group versus the routine group was(253.81±111.86)vs (217.82±103.24)μmol/L,(272.56±111.04)vs (198.90±102.36)μmol/L, and(280.12±111.09)μmol/L vs(191.97±101.81)μmol/L, respectively. The level of uric acid was significantly higher in the IDE group than in the routine group(P<0.05). (7) In month 6, 12 and 18, the SOD level in the IDE group versus that in the routine group was(154.45±39.96)vs (126.47±41.30)U/mL,(162.45±42.08)vs (119.36±39.71)U/mL, and(172.90±41.78)vs (112.17±37.17)U/mL, respectively. The SOD level was significantly higher in the IDE group than in the routine group(P<0.05). Conclusion IDE can improve the motor and non-motor symptoms in PD patients and slow down the progression of the disease. It has a certain neuroprotective effect on PD.

Magnetic resonance spectroscopy in patients with vascular cognitive impairment no dementia

ZHANG Xiaoqian, MENG Xiangshui, REN Qingguo, NAN Xiaomin, AN Panpan, SHUAI Xinyan, XIA Xiaona, WANG Xuan

Journal of Shandong University (Health Sciences). 2019, 57(4):  42-46.  doi:10.6040/j.issn.1671-7554.0.2018.1341

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Objective To explore the relevance between metabolic changes in the critical parts of brain and vascular cognitive impairment no dementia(VCIND)by analyzing the metabolite ratios in VCIND patients using magnetic resonance spectroscopy(MRS). Methods A total of 38 VCIND patients(VCIND group)and 44 healthy controls(control group)were involved. The N-acetyl aspartate(NAA), choline(Cho), creatine(Cr), NAA/Cr and Cho/Cr in the frontal lobe, temporal lobe, thalamus, hippocampus, basal ganglia and cingulate gyrus in the two groups were measured with 3.0T MRS. The above parameters in dominant hemisphere of the two groups were compared and statistically analyred. Results Compared with the control group, the VCIND group had significantly reduced NAA in the temporal lobe, hippocampus, thalamus, and cingulate gyrus, and decreased NAA/Cr ratio in the frontal lobe, hippocampus, thalamus and cingulate gyrus (P<0.05). There were no statistical differences in Cho and Cho/Cr in different parts in the dominant hemisphere between two groups(P>0.05). There were no significant differences in all of the parameters between the dominant and non-dominant hemisphere in the VCIND group. Conclusion NAA and NAA/Cr are sensitive indicators to detect VCIND. VCIND patients have extensive cerebral metabolic disorder, but in the dominant hemisphere, the metabolic changes were associated with VCIND in the frontal lobe, temporal lobe, hippocampus, thalamus, and cingulate gyrus. 山 东 大 学 学 报 (医 学 版)57卷4期 -张晓倩,等.磁共振波谱成像对检测非痴呆型血管性认知障碍的探讨 \=-

Association between apolipoprotein E gene polymorphism and cerebral microbleeds in ischemic cerebrovascular patients

SONG Yu, TIAN Shufen

Journal of Shandong University (Health Sciences). 2019, 57(4):  47-51.  doi:10.6040/j.issn.1671-7554.0.2018.1427

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Objective To investigate the association between apolipoprotein E genotype(ApoE)and cerebral microbleeds(CMBs)in ischemic cerebrovascular. Methods A total of 130 ischemic cerebrovascular patients treated in our hospital during Nov. 2016 and Nov. 2018 were randomly selected and divided into the CMBs group and non-CMBs group according to the results of susceptibility-weighted imaging(SWI). Clinical information and related laboratory tests were collected. The genotypes of ApoE were determined with polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). Results There were statistical differences in the frequency distributions of ApoE genotypes and alleles between the CMBs group and non-CMBs group (P<0.05). Logistic regression analysis showed that ApoE ε4 carrier (OR: 3.628, 95%CI 1.214~10.839), male (OR: 3.287, 95%CI 1.209~8.934) and carotid atherosclerosis (OR: 4.401, 95%CI 1.614~12.005) were the independent risk factors for CMBs. ApoE ε4 carriers had higher possibility of developing lobar CMBs. Conclusion Apolipoprotein E gene polymorphism is associated with cerebral microbleeds in ischemic cerebrovascular. ApoE ε4 carriers are more likely to develop CMBs than non-carriers.

Correlation between sleep architecture and attention and memory function in chronic insomniacs

SONG Limin, LU Shanshan, WANG Dawei, LÜ Xin, GAO Yuan, TANG Jiyou

Journal of Shandong University (Health Sciences). 2019, 57(4):  52-58.  doi:10.6040/j.issn.1671-7554.0.2018.452

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Objective To explore the correlation between sleep architecture and attention and memory function in chronic insomniacs. Methods A total of 31 patients with chronic insomnia disorder and 26 good sleepers were enrolled in this study. All subjects were assessed with Pittsburgh Sleep Quality Index(PSQI), Epworth Sleepiness Scale(ESS), Insomnia Severity Index(ISI)and polysomnography(PSG)to evaluate sleep quality, the severity of insomnia and sleep architecture. Attention and memory functions were detected with the Wechsler Memory Scale(WMS). Results Compared with good sleepers, chronic insomniacs had higher scores of PSQI, ESS and ISI. They showed longer sleep latency, higher percentages of non-rapid eye movement sleep 1 stage(NREM1%)and non-rapid eye movement sleep 2 stage(NREM2%), and more times of wake after sleep onset(WASO), but lower percentage of non-rapid eye movement 山 东 大 学 学 报 (医 学 版)57卷4期 -宋立敏,等.慢性失眠患者睡眠结构与注意力、记忆的相关性 \=-sleep 3 stage(NREM3%)(all P<0.05). In chronic insomniacs, the worsened attention could be seen from the intelligence test and visual memory span tests; the loss of short-time memory could be detected with associative learning and logical memory tasks. Multivariate Logistic regression analysis indicated that PSQI was correlated with intelligence and visual memory(P<0.05), NREM1% was correlated with associative learning and logical memory(P<0.05), and NREM3% was correlated with logical memory(P<0.01). Conclusion The sleep architecture is disordered in chronic insomniacs. The losses of attention and short-time memory are primarily correlated with PSQI score, increased NREM1% and decreased NREM3%.

A study of sleep quality of patients with breast cancer and influencing factors

LUAN Na, LIU Liyuan, WANG Fei, ZHOU Fei, XIANG Yujuan, HUANG Shuya, ZHENG Chao, YU Lixiang, YU Zhigang

Journal of Shandong University (Health Sciences). 2019, 57(4):  59-64.  doi:10.6040/j.issn.1671-7554.0.2019.042

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Objective To investigate the sleep quality of patients with breast cancer and analyze the influencing factors. Methods A total of 100 female breast cancer patients who received breast surgery during Jul. and Nov. 2018 were selected as the case group, and 200 healthy women who received health screening during the same period were selected as the control group. The demographic characteristics and sleep quality of the subjects were collected using a self-designed general data questionnaire and the Pittsburgh Sleep Quality Index(PSQI). Patients disease-related information was obtained from the medical records. The influencing factors of sleep quality were analyzed with linear regression analysis. Results The case group had lower sleep quality than control group(4.99±3.06 vs 6.46±3.58, t=3.512, P<0.001), and 67.0% of the patients and 44.5% of the healthy controls had poor sleep quality(χ2=13.522, P<0.001). Patients who underwent mastectomy had poorer sleep quality than those who did not receive surgery (B=2.81, Beta=0.38, 95% CI: 0.77~4.85, P=0.007)and those who underwent breast-conserving surgery (B=2.92, Beta=0.40, 95% CI: 1.40~4.45, P<0.001). Conclusion Breast cancer patients have poorer sleep quality than healthy women. Mastectomy is an important influencing factor of sleep quality, indicating that attention should be paid to the evaluation of postoperative sleep quality.

Application value of combined detection of tumor-associated autoantibodies in the diagnosis of colorectal cancer

GENG Xu, ZHANG Lu, LI Ming, LU Zhiming

Journal of Shandong University (Health Sciences). 2019, 57(4):  65-71.  doi:10.6040/j.issn.1671-7554.0.2019.076

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Objective To explore the application value of combined detection of tumor-associated autoantibodies in the diagnosis of colorectal cancer(CRC). Methods The serum specimens and clinical data of CRC patients(CRC group)and precancerous patients(precancerous group)treated in our hospital during May 2017 and November 2017, and the information of healthy controls(control group)were collected. The apurinic/apyrimidinic endonuclease 1(APE1), microtubule associated protein RP/EB family, member 1(MAPRE1)and New York esophageal squamous cell carcinoma 1(NY-ESO-1)autoantibodies levels were detected with enzyme-linked immunosorbent assay(ELISA). The diagnostic efficacies of three autoantibodies and traditional indicators(CEA and CA-199)were analyzed with receiver operating characteristic curve(ROC). Results There were significant differences in the levels of APE1 AAbs, MAPRE1 AAbs and NY-ESO-1 AAbs among the three groups. The area under the curve(AUC)of the 3 autoantibodies(0.772, 95%CI: 0.688-0.856; 0.794, 95%CI: 0.716-0.873; 0.854, 95%CI: 0.778-0.929)were higher than that of CA-199(0.571, 山 东 大 学 学 报 (医 学 版)57卷4期 -耿旭,等.多种肿瘤自身抗体对结直肠癌诊断的应用价值 \=-95%CI: 0.477-0.664, P<0.05). The AUC of NY-ESO-1 AAbs was higher than that of CEA(0.720, 95%CI: 0.639-0.801, P<0.05). The AUC of NY-ESO-1 AAbs + CEA(0.889, 95%CI: 0.829-0.949)was higher than that of NY-ESO-1 AAbs (P=0.03). The AUC of APE1 AAbs+NY-ESO-1 AAbs+CEA(0.927, 95%CI: 0.885-0.970)was statistically higher than that of NY-ESO-1 AAbs+CEA (P=0.04). Conclusion The combined detection of APE1 AAbs, NY-ESO-1 AAbs and CEA can significantly improve the diagnostic efficacy of colorectal cancer, and it is worth clinical application.

Minimum effective concentration of ropivacaine for subarachnoid block in 30 cases of anorectal day surgery

HUANG Yuting, LIU Huiying, ZHENG Junyi

Journal of Shandong University (Health Sciences). 2019, 57(4):  72-76.  doi:10.6040/j.issn.1671-7554.0.2018.1160

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Clinical analysis of 86 cases of testicular tumor

HU Jialin, LÜ Jiaju, TAN Yueqing, GUAN Mingde, MAO Changlin, DING Sentai

Journal of Shandong University (Health Sciences). 2019, 57(4):  77-83.  doi:10.6040/j.issn.1671-7554.0.2018.1467

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Adverse outcome and correlative factors analysis of initial noninvasive continuous positive airway pressure failure in 153 very/extremely low birth weight infants

WANG Li, CHEN Dong, YU Yonghui, DONG Xiaoyu, CHEN Yao, LI Gang

Journal of Shandong University (Health Sciences). 2019, 57(4):  84-90.  doi:10.6040/j.issn.1671-7554.0.2018.1321

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Objective To analysis the cause and adverse outcome of initial noninvasive continuous positive airway pressure failure in very/extremely low weight(VLBW/ELBW)infants. Methods A total of 153 VLBW/ELBW infants with the birth wight <1.50 kg and gestational age<32.0 weeks in Shandong Provincial Hospital Affiliated to Shandong 山 东 大 学 学 报 (医 学 版)57卷4期 -王莉,等.153例极/超低出生体重儿初始无创持续气道正压通气失败的不良预后及关联性因素分析 \=- University from January 1, 2016 to September 30, 2017 were enrolled. The infants were divided into noninvasive-S group(n=126), in which the noninvasive support was successful, and noninvasive-F group(n=27), in which the noninvasive support was failed. In-hospital outcomes, including survival rate, pneumorrhagia, pneumothorax, intracranial hemorrhage, late-onset sepsis(LOS), neonatal necrotizing enterocolitis(NEC), bronchopulmonary dysplasia(BPD), retinopathy of prematurity(ROP)and extrauterine growth retardation(EUGR), were compared between the two groups. Logistic regression models were used to further investigate the perinatal factors associated with noninvasive failure and its correlation with the adverse outcomes during hospitalization of the infants. Results The gestation age, birth weight, perinatal asphyxia and EOS were statistical different between the two groups(P<0.05), and the Logistic regression analysis showed that birth weight and perinatal asphyxia were associated with noninvasive failure(P<0.05). The ROC analysis showed that birth weight <1.17 kg was the most significant cutoff value for the development of noninvasive failure with sensibility being 0.78, specificity being 0.71, and area under the curve being 0.80, P<0.001. Logistic regression analysis for short-outcome showed that the death rate, pneumorrhagia, LOS and ROP were statistical different between the two groups(P<0.05). The two crucial causes of death were pneumorrhagia and intracranial hemorrhage in noninvasive-F group, and the gestational age of the dead infants caused by pneumorrhagia was 29.6(26.8, 30.3)weeks and birth weight was 0.80(0.74, 1.11)kg. Conclusion The rate of noninvasive failure was 17.6% in VLBW /ELBW infants, and lower birth weight(<1.17 kg)and perinatal asphyxia are associated with noninvasive failure. Noninvasive failure in preterm infants is associated with death, pneumorrhagia, LOS, and ROP. The leading causes of death are pneumorrhagia, especially for the infants with birth weight ≤0.80 kg, and intracranial hemorrhage.

Diagnostic value of serum exosomal miR-20b-5p for non-small cell lung cancer

WEI Ping, DU Lutao, WANG Qing, ZHAN Yao, XIE Yujiao, ZHANG Shujun, DUAN Weili, WANG Chuanxin

Journal of Shandong University (Health Sciences). 2019, 57(4):  91-96.  doi:10.6040/j.issn.1671-7554.0.2019.028

Abstract ( 443 )   PDF (2117KB) ( 70 )   Save

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Objective To explore the diagnostic value of serum exosomal miR-20b-5p for non-small cell lung cancer(NSCLC). Methods Serum samples of 144 NSCLC patients(NSCLC group)and 138 healthy controls(healthy group)were collected. Exosomes in the serum were extracted and characterized, and miRNA was abstracted. The expression of exosomal miR-20b-5p in the NSCLC group and healthy control group were compared with RT-qPCR. The diagnostic value of exosomal miR-20b-5p was evaluated with receiver operating characteristic(ROC)curve. Results Purified exosomes had typical features. The expression of miR-20b-5p was significantly higher in the NSCLC group than in the healthy control group (P<0.001). The area under the ROC curve(AUC)was 0.849(95%CI:0.802~0.889). The diagnostic sensitivity was 73.61%, and the specificity was 70.29%. Conclusion Serum exosomal miR-20b-5p is highly expressed in NSCLC with highly clinical diagnostic value. It can serve as a new biomarker to assist the diagnosis of NSCLC.

An analysis of the mutation of MSX1 gene in a non-syndromic tooth agenesis family

DING Tingting, ZOU Dong, LIU Haochen

Journal of Shandong University (Health Sciences). 2019, 57(4):  97-100.  doi:10.6040/j.issn.1671-7554.0.2018.1302

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Objective To search for the disease-causing gene in a family with non-syndromic tooth agenesis. Methods A family with non-syndromic tooth agenesis was recruited from Beijing Childrens Hospital. The clinical data were collected and genomic DNAs were extracted from peripheral blood lymphocytes. The common pathogenic genes of tooth agenesis were detected, and the bioinformatic and phenotypic information of the suspected mutation was analyzed. Results A de novo MSX1 missense mutation(c.547C>A)was identified, which resulted in the substitution of Gln at residue 183 for Lys(p.Q183K); residue 105 was located in the highly conserved region of the MSX1 protein. Mutation pathogenicity predictions showed that the mutation was highly pathogenic. Conclusion A de novo MSX1 missense mutation is identified in a patient with non-syndromic tooth agenesis, which confirms the relationship between MSX1 mutation and non-syndromic tooth agenesis and extends the mutation spectrum of the MSX1 gene.

Comparison of the efficiency of rectangular attachment and optimized attachment in the distal movement of canine on invisalign aligner

JU Bo, LI Deshui, LIU Panpan, GUO Jing

Journal of Shandong University (Health Sciences). 2019, 57(4):  101-105.  doi:10.6040/j.issn.1671-7554.0.2019.092

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Objective To compare the efficiency of the rectangular attachment and optimized attachment in the distal movement of canine on invisalign aligner. Methods Altogether 16 individuals who received first premolars extraction surgery and invisalign aligner treatment were enrolled in this study. The rectangular attachment and optimized attachment were placed on the 2 lower canines of each individual randomly using Clincheck. The 3D digital models obtained at the initial and the end of 16th aligners were aligned with Geomagic studio 12. Then the mesiodistal, vertical and labiolingual movement, inclination, angulation and rotation of canines were measured and calculated in the same coordinate system. All measurement indexes were analyzed using SPSS 20.0 software. Results No statistic differences were observed in the mesiodistal, vertical and labiolingual movement (P>0.05). The rectangular attachment was approximately 1.46 times as effective as optimized attachment in the control of angulation. There was no significant difference in torque changes between the two groups. Highly significant difference (P<0.01) was found in terms of rotation. The efficiency of holistic teeth movement in the rectangular attachment group was 69.23% higher than that in the optimized attachment group. Conclusion In teeth extraction, the rectangular attachment is more effective in canine bodily movement than the optimized attachment.

Association between trajectory of blood pressure and risk of coronary heart disease in middle aged and elderly population

LIN Weiqiang, WANG Chunxia, LI Mingzhuo, SUN Xiubin, LIU Yanxun, XUE Fuzhong, YUAN Zhongshang

Journal of Shandong University (Health Sciences). 2019, 57(4):  106-112.  doi:10.6040/j.issn.1671-7554.0.2019.019

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Analysis of the proportion of drug expenses and medical insurance payment based on hospitalization expenses of five cancer patients

YANG Yuxia, ZANG Sujie, LENG Anli, WANG Jian

Journal of Shandong University (Health Sciences). 2019, 57(4):  113-118.  doi:10.6040/j.issn.1671-7554.0.2018.1401

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Objective To analyze the features and variation trends of the hospitalization expenses, drug proportions and payment methods of medical insurances among cancer inpatients between 2013 and 2017, in order to provide references for eliminating drug additions, adjusting medical service prices, and accelerating the establishment of new compensation mechanisms for public hospitals. Methods Patients whose first diagnosis was gastric cancer, lung cancer, colorectal cancer, esophageal cancer, or breast cancer were selected from a tertiary hospital in Shandong Province from 2013 to 2017. The characteristics of hospitalization expenses were described. The trends in different years were analyzed with one-way ANOVA and χ² test. Results A total of 20 138 inpatients were involved. In 2017, patients with esophageal cancer had the highest hospitalization expenses(34 137.96 yuan). From 2013 to 2017, the drug proportions were generally on the decline, but there was no obvious decrease in hospitalization expenses. On the contrary, the hospitalization expenses of lung cancer patients increased significantly. In addition, there was a large gap in the compensation proportions among the three types of medical insurances. Conclusion The annual decrease of drug proportion does not bring significant reduction to hospitalization expenses. The economic burden of cancer inpatients is still heavy. The drug proportion should continue to be reduced based on the controlling of the total hospitalization expenses. The gap in the compensation proportion of various types of medical insurances should be narrowed to effectively alleviate the financial burden of patients.