Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (3): 13-18.doi: 10.6040/j.issn.1671-7554.0.2018.661

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Effects of Physalin B on proliferation, migration and apoptosis of human non-small cell lung cancer cells

WANG Wei, LIU Yongzheng, LI Ling   

  1. Oncology Department, The Second Hospital of University of South China, Hengyang 421001, Hunan, China
  • Published:2022-09-27

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Abstract: Objective To investigate the effects of Physalin B(PB)on proliferation, migration and apoptosis of A549 cells in non-small cell lung cancer(NSCLC), and preliminarily explore its mechanism. Methods The A549 cells were divided into different concentrations(2.5, 5, 10, 20, 40 μmol/L)of PB groups, control group and blank group. The inhibition rate of PB on A549 cells proliferation was detected by real-time cell analyzer(RTCA)and CCK-8 assay, cell migration was examined by transwell chamber assay, the apoptosis and cycle of PB on A549 cells were detected by flow cytometry and the effect of PB on the expression of related proteins was detected by Western blotting. Results PB with 2.5, 5, 10, 20 and 40 μmol/L significantly inhibited the proliferation of A549 cells in a dose-dependent manner. The cell migration ability of 10 and 20 μmol/L PB groups was significantly lower than that of control group. After treatment with 10 and 20 μmol/L PB, the apoptosis rate of A549 cells increased in a dose-dependent manner. Compared with control group, the proportion of G2/M phase cells increased and the proportion of G0/G1 phase cells decreased in 10 and 20 μmol/L PB groups. Compared with the control group, the expression level of Akt protein decreased while the expression levels of Caspase-9 and PTEN increased in 10 and 20 μmol/L PB groups. Conclusion PB can inhibit the proliferation of A549 cells, induce cell apoptosis and cell cycle arrest, which may be related to the regulation of cell cycle related protein expression.

Key words: Non-small cell lung cancer, Physalin B, Proliferation, Apoptosis, Transfer

CLC Number: 

  • R734.2
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