Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (3): 13-18.doi: 10.6040/j.issn.1671-7554.0.2018.661

Previous Articles    

Effects of Physalin B on proliferation, migration and apoptosis of human non-small cell lung cancer cells

WANG Wei, LIU Yongzheng, LI Ling   

  1. Oncology Department, The Second Hospital of University of South China, Hengyang 421001, Hunan, China
  • Published:2022-09-27

PDF (PC)

19

Abstract: Objective To investigate the effects of Physalin B(PB)on proliferation, migration and apoptosis of A549 cells in non-small cell lung cancer(NSCLC), and preliminarily explore its mechanism. Methods The A549 cells were divided into different concentrations(2.5, 5, 10, 20, 40 μmol/L)of PB groups, control group and blank group. The inhibition rate of PB on A549 cells proliferation was detected by real-time cell analyzer(RTCA)and CCK-8 assay, cell migration was examined by transwell chamber assay, the apoptosis and cycle of PB on A549 cells were detected by flow cytometry and the effect of PB on the expression of related proteins was detected by Western blotting. Results PB with 2.5, 5, 10, 20 and 40 μmol/L significantly inhibited the proliferation of A549 cells in a dose-dependent manner. The cell migration ability of 10 and 20 μmol/L PB groups was significantly lower than that of control group. After treatment with 10 and 20 μmol/L PB, the apoptosis rate of A549 cells increased in a dose-dependent manner. Compared with control group, the proportion of G2/M phase cells increased and the proportion of G0/G1 phase cells decreased in 10 and 20 μmol/L PB groups. Compared with the control group, the expression level of Akt protein decreased while the expression levels of Caspase-9 and PTEN increased in 10 and 20 μmol/L PB groups. Conclusion PB can inhibit the proliferation of A549 cells, induce cell apoptosis and cell cycle arrest, which may be related to the regulation of cell cycle related protein expression.

Key words: Non-small cell lung cancer, Physalin B, Proliferation, Apoptosis, Transfer

CLC Number: 

  • R734.2
[1] Jemal A, Siegel R, Xu JQ, et al. Cancer statistics, 2010[J]. CA Cancer J Clin, 2010, 60(5): 277-300.
[2] Chen WQ, Zheng RS, Zeng HM, et al. Epidemiology of lung cancer in China[J]. Thorac Cancer, 2015, 6(2): 209-215.
[3] Verdecchia A, Francisci S, Brenner H, et al. Recent cancer survival in Europe: a 2000-02 period analysis of EUROCARE-4 data[J]. Lancet Oncol, 2007, 8(9): 784-796.
[4] Bravo LE, García LS, Collazos PA. Cancer survival in Cali, Colombia: a population-based study, 1995-2004[J]. Colomb Med, 2014, 45(3): 110-116.
[5] Jänne PA, Johnson BE. Effect of epidermal growth factor receptor tyrosine kinase domain mutations on the outcome of patients with non-small cell lung cancer treated with epidermal growth factor receptor tyrosine kinase inhibitors[J]. Clin Cancer Res, 2006, 12(14 Pt 2): 4416s-4420s.
[6] 杨燕军, 陈梅果, 胡玲, 等. 苦蘵的化学成分研究[J]. 中国药学杂志, 2013, 48(20): 1715-1718. YANG Yanjun, CHEN Meiguo, HU Ling, et al. Chemical constituents of whole plant of physalis angulata L[J]. Chinese Pharmaceutical Journal, 2013, 48(20): 1715-1718.
[7] Cragg GM, Newman DJ. Plants as a source of anti-cancer agents[J]. J Ethnopharmacol, 2005, 100(1/2): 72-79.
[8] Makino B, Ohya J, Yamamura H, et al. Cytotoxic activity of physalins possessing modified skeletal structures against HeLa cells[J]. Pharmazie, 2003, 58(1): 70-71.
[9] Wu SJ, Ng LT, Chen CH, et al. Antihepatoma activity of Physalis angulata and P. peruviana extracts and their effects on apoptosis in human Hep G2 cells[J]. Life Sci, 2004, 74(16): 2061-2073.
[10] Hsieh WT, Huang KY, Lin HY, et al. Physalis angulata induced G2/M phase arrest in human breast cancer cells[J]. Food Chem Toxicol, 2006, 44(7): 974-983.
[11] Sá MS, de Menezes MN, Krettli AU, et al. Antimalarial activity of physalins B, D, F, and G[J]. J Nat Prod, 2011, 74(10): 2269-2272.
[12] Jacobo-Herrera NJ, Bremner P, Marquez N, et al. Physalins from Witheringia solanacea as modulators of the NF-kappaB cascade[J]. J Nat Prod, 2006, 69(3): 328-331.
[13] Vandenberghe I, Créancier L, Vispé S, et al. Physalin B, a novel inhibitor of the ubiquitin-proteasome pathway, triggers NOXA-associated apoptosis[J]. Biochem Pharmacol, 2008, 76(4): 453-462.
[14] Hsu CC, Wu YC, Farh L, et al. Physalin B from Physalis angulata triggers the NOXA-related apoptosis pathway of human melanoma A375 cells[J]. Food Chem Toxicol, 2012, 50(3/4): 619-624.
[15] Ye YC, Tang XY, Sun ZZ, et al. Upregulated WDR26 serves as a scaffold to coordinate PI3K/ AKT pathway-driven breast cancer cell growth, migration, and invasion[J]. Oncotarget, 2016, 7(14): 17854-17869.
[16] Cai J, Xu LJ, Tang HJ, et al. The role of the PTEN/PI3K/Akt pathway on prognosis in epithelial ovarian cancer: a meta-analysis[J]. Oncologist, 2014, 19(5): 528-535.
[17] Chen JZ, O'Donoghue A, Deng YF, et al. The effect of lycopene on the PI3K/Akt signalling pathway in prostate cancer[J]. Anticancer Agents Med Chem, 2014, 14(6): 800-805.
[18] Khan KH, Yap TA, Yan L, et al. Targeting the PI3K-AKT-mTOR signaling network in cancer[J]. Chin J Cancer, 2013, 32(5): 253-265.
[19] Carnero A, Blanco-Aparicio C, Renner O, et al. The PTEN/PI3K/AKT signalling pathway in cancer, therapeutic implications[J]. Curr Cancer Drug Targets, 2008, 8(3): 187-198.
[20] Jiang BH, Liu LZ. PI3K/PTEN signaling in tumorigenesis and angiogenesis[J]. Biochim Biophys Acta, 2008, 1784(1): 150-158.
[21] Oda K, Stokoe D, Taketani Y, et al. High frequency of coexistent mutations of PIK3CA and PTEN genes in endometrial carcinoma[J]. Cancer Res, 2005, 65(23): 10669-10673.
[22] Jiang BH, Zheng JZ, Aoki M, et al. Phosphatidylinositol 3-kinase signaling mediates angiogenesis and expression of vascular endothelial growth factor in endothelial cells[J]. Proc Natl Acad Sci U S A, 2000, 97(4): 1749-1753.
[23] Jeong YJ, Choi Y, Shin JM, et al. Melittin suppresses EGF-induced cell motility and invasion by inhibiting PI3K/Akt/mTOR signaling pathway in breast cancer cells[J]. Food Chem Toxicol, 2014, 68: 218-225. doi:10.1016/j.fct.2014.03.022.
[24] Zhang H, Zhao B, Huang C, et al. Melittin restores PTEN expression by down-regulating HDAC2 in human hepatocelluar carcinoma HepG2 cells[J]. PLoS One, 2014, 9(5): e95520. doi:10.1371/journal.pone.0095520.
[1] WANG Xiao-Lei, ZHANG Hai-Thao, ZHANG Hui, GUO Cheng-Hao. Protective effects of shuxuening injecta on vascular  endothelial  cell injury in vitro due to iodine excesses [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 38-.
[2] LU Xiang-Dong, YANG Wei, XU Guang-Meng, QU Yuan-Ming. Expression and role of PPAR-γin meningiomas and troglitazone induced meningiomas cell apoptosis in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 65-.
[3] ZHAO Ge, ZOU Cunhua, SONG Dongdong, ZHAO Shuping. Effects of tanshinone IIA on the proliferation and apoptosis of endometrial carcinoma cells [J]. Journal of Shandong University (Health Sciences), 2022, 60(9): 53-58.
[4] ZHANG Zhenwei, LI Jia, CHEN Keming. IGF2BP2/m6A/ITGA5 signal axis regulates the proliferation and migration of renal clear cells [J]. Journal of Shandong University (Health Sciences), 2022, 60(9): 74-84.
[5] QIN Jing, YANG Fei, CHEN Qian, XIA Handai, LIU Yanguo, WANG Xiuwen. A network meta-analysis of first-line treatment options for patients with advanced driver-gene wild-type and PD-L1 negative non-squamous non-small cell lung cancer [J]. Journal of Shandong University (Health Sciences), 2022, 60(7): 74-82.
[6] Lei YAN,Zijiang CHEN. Treatment of adenomyosis complicated with infertility [J]. Journal of Shandong University (Health Sciences), 2022, 60(7): 43-47.
[7] WU Richao, LIU Hong, WANG Ze, GAO Shanshan, LI Xiufang, SHI Yuhua. Pregnancy outcomes of in vitro fertilization-intracytoplasmic sperm injection-embryo transfer in women 40 years and above [J]. Journal of Shandong University (Health Sciences), 2022, 60(6): 35-39.
[8] LIU Min, ZHANG Yuchao, MA Xiaoli, LIU Xinyu, SUN Lu, ZUO Dan, LIU Yuantao. Effects of orphan nuclear receptor NR4A1 on H2O2 induced injury of mouse renal podocytes [J]. Journal of Shandong University (Health Sciences), 2022, 60(5): 16-21.
[9] SHEN Xiaochang, SUN Yiqing, YAN Lei, ZHAO Xingbo. Expression of aryl hydrocarbon receptor nuclear translocator-like 2 in endometrial cancer [J]. Journal of Shandong University (Health Sciences), 2022, 60(5): 74-80.
[10] CHEN Zhaobo, FANG Min, XUE Haoran, LIU Chunyan. Deubiquitinase USP35 promotes the cells migration and invasion of non-small cell lung cancer [J]. Journal of Shandong University (Health Sciences), 2022, 60(4): 30-37.
[11] SONG Tian, FU Linlin, WANG Qiumin, YANG Xiao, WANG Ying, BIAN Yuehong, SHI Yuhua. Expression of fatty acid transport protein 1 in granulosa cells of patients with polycystic ovary syndrome [J]. Journal of Shandong University (Health Sciences), 2022, 60(2): 22-26.
[12] LI Hui, JIANG Chaoyang, LIU Yan, ZHANG Man. Effects and mechanism of histone deacetylase SIRT1 controlled macrophage apoptosis induced by oxidized low density lipoprotein [J]. Journal of Shandong University (Health Sciences), 2022, 60(1): 6-12.
[13] Yuhua SHI,Qiumin WANG,Dan QI. Frontier research hotspot and progress in assisted reproductive technology [J]. Journal of Shandong University (Health Sciences), 2021, 59(9): 97-102.
[14] Xiaohui DENG,Ling GUO. Applications and progress of immunotherapy in repeated implantation failure [J]. Journal of Shandong University (Health Sciences), 2021, 59(8): 32-37.
[15] CHU Yan, LIU Duanrui, ZHU Wenshuai, FAN Rong, MA Xiaoli, WANG Yunshan, JIA Yanfei. Expressions of DNA methyltransferases in gastric cancer and their clinical significance [J]. Journal of Shandong University (Health Sciences), 2021, 59(7): 1-9.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd