Journal of Shandong University (Health Sciences) ›› 2021, Vol. 59 ›› Issue (2): 7-13.doi: 10.6040/j.issn.1671-7554.0.2020.1469

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Construction and significance of a rat model of OSAHS with chronic intermittent hypoxia

WANG Haipeng1, ZOU Juanjuan2, GAO Chunmiao1, WANG Xiao1, WANG Yan2, LI Yanzhong2   

  1. 1. Department of Otorhinolaryngology Head and Neck Surgery, Zibo Central Hospital, Zibo 255036, Shandong, China;
    2. Department of Otorhinolaryngology, Qilu Hospital Cheeloo College of Medicine, Shandong University, National Health Commission Key Laboratory of Otorhinolaryngology(Shandong University), Jinan 250012, Shandong, China
  • Published:2021-03-05

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Abstract: Objective To construct a rat model of obstructive sleep apnea hypopnea syndrome(OSAHS)with chronic intermittent hypoxia. Methods A color touch screen, a programmable logic controller(PLC)automatic control, and an automated data acquisition and monitoring system were used to assemble the equipment needed to construct the experimental model. The hypoxic chamber was composed of the main engine, the hypoxic chamber and the gas path control. A total of 30 rats were randomly divided into two groups, normoxic control(CON)group and CIH model(CIH). After 9 weeks of normal feeding, the rats were sacrificed, and liver samples were collected for hematoxylin and eosin(HE)staining, transmission electron microscopy, and Oil Red “O” staining. Results Morphological changes: HE staining of the CIH group showed lipid accumulation and lipid vacuole formation leading to hepatocyte steatosis. Microscopy of the ultrastructure showed large clusters of fat droplets and appearance of large numbers of autophagosomes and lysosomes, focal accumulation of glycogen in the rough endoplasmic reticulum, and sparse microvilli in the bile canaliculi. these were rarely seen in the CON group. The CIH group showed obvious morphological changes. Oil Red O staining results: the difference in IOD/Area was statistically significant between the CIH group and CON group(The test statistic=-56.308, P=0.001). Hepatocytes in the CIH group were obviously stained red. Conclusion The rat model of OSAHS with chronic intermittent hypoxia is successful, reliable and effective, which is consistent with the pathophysiological characteristics of OSAHS, and lays a solid foundation for further research on the pathogenesis of abnormal function of various tissues and organs in rats and the possible therapeutic effects of related drugs.

Key words: Sleep apnea syndrome, Hypoxia, Animal, Model, Rats

CLC Number: 

  • R766.3
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