Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (5): 22-30.doi: 10.6040/j.issn.1671-7554.0.2022.0016

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Evening primrose oil resists oxidative stress in the ovaries of rats with polycystic ovary syndrome

HU Na1,2, SUN Miao1,2, XING Shasha1,2, XU Danxia1,3, HAI Xiaoming1,3, MA Ling1,3, YANG Li4, MIAN Yuchen1,2, HE Rui1,2, CHEN Dongmei3, MA Huiming1,2   

  1. 1. Key Laboratory of Fertility Preservation and Maintenance(Ministry of Education), Ningxia Medical University, Yinchuan 750004, Ningxia, China;
    2. Department of Human Anatomy and Tissue Embryology, School of Basic Medical Science, Ningxia Medical University, Yinchuan 750004, Ningxia, China;
    3. Ningxia Key Laboratory of Stem Cell and Regenerative Medicine, The General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia, China;
    4. Experimental Animal Center of Ningxia Medical University, Yinchuan 750004, Ningxia, China
  • Published:2022-06-01

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Abstract: Objective To investigate the effects of evening primrose oil(EPO)on the hormonal, metabolic and oxidation levels in rats with polycystic ovary syndrome(PCOS)induced by high-fat diet combined with letrozole, and to explore the expression of NAD-dependent deacetylase sirtuin-1(SIRT1)/Forkhead box O1(FoxO1)signaling pathway in the ovarian tissue. Methods A total of 72 rats were divided into 6 groups, with 12 as the normal control group, and the other 60 rats were fed high-fat diet(40%)for 8 weeks. In the last 3 weeks of the feeding program, letrozole at a concentration of 1 mg/kg/d was added to prepare PCOS models. After the models were established, the serum hormonal levels in the tail of rats were analyzed, and 2 rats with large deviation were removed from each group. Plus the normal control group, the PCOS models were divided into 5 groups, with 10 rats in each group: PCOS model group, metformin group, EPO low-dose group, EPO medium-dose group, and EPO high-dose group. The serum fasting plasma glucose(FPG), serum insulin(FINS), testosterone(T), luteinizing hormone(LH), follicle stimulating hormone(FSH), superoxide Dismutase(SOD), total antioxidant capacity(T-AOC)and malondialdehyde(MDA)of each group were detected with ELISA and biochemical method. The ovarian morphology was observed with hematoxylin and eosin(HE)staining. The locations and expressions of SIRT1/FoxO1 pathway, SOD1 and glutathione S-transferase(GST), SIRT1 and FoxO1 were detected with immunohistochemistry and Western blotting. Results Compared with the blank control group, the PCOS model group had significantly increased weight, FPG, FINS, T, LH, FSH(P<0.05), significantly decreased SOD and T-AOC(P<0.05), and significantly increased MDA(P<0.01). ELISA results showed that after EPO and metformin treatment, the weight, FPG, FINS and MDA decreased(P<0.05), but SOD increased(P<0.05)compared with the PCOS model group. Compared with PCOS model group, the metformin group, medium-dose and high-dose groups had significantly increased T-AOC(P<0.05), but significantly decreased T and LH(P<0.05). Compared with the PCOS model group, the metformin group and high-dose group had significantly decreased FSH level(P<0.05). Immunohistochemical results showed that SOD1, GST and SIRT1 were positively expressed in granulosa cells, mesenchymal cells, primordial follicles and corpus luteum. Western blotting results showed that compared with the blank control group, the PCOS model group had significantly increased expressions of SIRT1, FoxO1, P -FoxO1, P-FoxO1/FoxO1, SOD1 and GST(P<0.05). Compared with the PCOS model group, the high-dose group had significantly decreased levels of SOD1, GST and P-FoxO1(P<0.05); the medium-dose group had significantly decreased levels of SIRT1 and FoxO1(P<0.05). Conclusion EPO affects the levels of oxidative factors and hormones in the serum of PCOS model rats, inhibits the expression of SIRT1/FoxO1 signaling pathway, enhances the antioxidant capacity of ovarian tissue and improve the oxidative stress.

Key words: Evening primrose oil, SIRT1/FoxO1 signaling pathway, Oxidative stress, Polycystic ovarian syndrome, Rats

CLC Number: 

  • R711.75
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