Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (2): 14-21.doi: 10.6040/j.issn.1671-7554.0.2021.1290

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Protective effect of taurine on cognitive deficit induced by 1-bromopropane

ZHAO Huiwen, XU Lin, SHAN Shan, ZHAO Xiulan   

  1. Department of Toxicology and Nutrition, School of Public Health, Shandong University, Jinan 250012, Shandong, China
  • Published:2022-01-25

Abstract: Objective To explore the protective effect of taurine on cognitive deficit induced by 1-bromopropane(1-BP). Methods A total of 60 SPF adult male Wistar rats were randomly divided into control group, 1-BP group, low-dose taurine intervention group, high-dose taurine intervention group and taurine control group according to body weight. 1-BP was dissolved in corn oil and orally administrated at the dose of 800 mg/kg.bw. The spatial learning and memory function of rats were detected with Morris water maze(MWM)15-20 days after the administration of 1-BP. On Day 21, the rats were sacrificed and the cerebral cortex and hippocampus were separated immediately. The content of taurine was determined with high-performance liquid chromatography(HPLC). The expressions of oxidative phosphorylation protein complex of mitochondria and apoptosis related proteins in brains were detected with Western blotting. Results Morris water maze showed that compared with the control group, 1-BP group had longer escape latency, and decreased frequencies of crossing platform(P<0.01); compared with 1-BP group, the low-dose and high-dose taurine intervention groups had shorter escape latency and increased frequencies of crossing platform(P<0.01). The HPLC results showed that compared with the control group, the 1-BP group had significantly decreased taurine contents in cerebral cortex and hippocampus(P<0.001, P<0.05); compared with 1-BP group, the low-dose and high-dose taurine intervention groups had significantly increased taurine level(P<0.001, P<0.01). Compared with the control group, the 1-BP group had significantly decreased expression of mitochondrial oxidative phosphorylation protein complex(OXPHOS)and Bcl-2(P<0.01, P<0.05), but significantly increased expressions of BAX and cleaved capase-3(P<0.05, P<0.01). The alteration trends of OXPHOS and apoptosis related proteins in low-dose and high-dose taurine intervention groups were significantly reversed(P<0.01, P<0.05, P<0.001). Conclusion Taurine supplementation could inhibit neuronal apoptosis, maintain the normal function of mitochondria, and improve the learning and memory dysfunction caused by 1-BP.

Key words: 1-bromopropane, Taurine, Rats, Cognitive deficit

CLC Number: 

  • R994.6
[1] 张意. 1-溴丙烷神经毒性作用机制及生物标志研究进展[J]. 卫生研究, 2017, 46(3): 505-508.
[2] Wang TH, Wu ML, Wu YH, et al. Neurotoxicity associated with exposure to 1-bromopropane in golf-club cleansing workers [J]. Clin Toxicol, 2015, 53(8): 823-826.
[3] 李卫华, 王强毅, 市原学, 等. 1-溴丙烷对接触工人神经毒性的剂量-效应关系[J]. 中华劳动卫生职业病杂志, 2010, 28(7): 488-493. LI Weihua, WANG Qiangyi, ICHIHARA Gaku, et al. Exposure to 1-bromopropane causes dose-dependent neurological abnormalities in workers [J]. Chinese Journal of Industrial Hygiene and Occupational Diseases, 2010, 28(7): 488-493.
[4] Kumari N, Prentice H, Wu JY. Taurine and its neuroprotective role [J]. Adv Exp Med Biol, 2013, 775: 19-27. doi:10.1007/978-1-4614-6130-2_2.
[5] Wu JY, Wu H, Jin Y, et al. Mechanism of neuroprotective function of taurine [J]. Adv Exp Med Biol, 2009, 643: 169-179. doi: 10.1007/978-0-387-75681-3_17.
[6] Hernández-Benítez R, Pasantes-Morales H, Salda(~overn)a IT, et al. Taurine stimulates proliferation of mice embryonic cultured neural progenitor cells [J]. J Neurosci Res, 2010, 88(8): 1673-1681.
[7] El Idrissi A, Shen CH, L'amoreaux WJ. Neuroprotective role of taurine during aging [J]. Amino Acids, 2013, 45(4): 735-750.
[8] Wu JY, Prentice H. Role of taurine in the central nervous system [J]. J Biomed Sci, 2010, 17(Suppl 1): S1.
[9] Lambert IH, Kristensen DM, Holm JB, et al. Physiological role of taurine: from organism to organelle [J]. Acta Physiol(Oxf), 2015, 213(1): 191-212.
[10] Vitvitsky V, Garg SK, Banerjee R. Taurine biosynthesis by neurons and astrocytes [J]. J Biol Chem, 2011, 286(37): 32002-32010.
[11] Oja SS, Saransaari P. Open questions concerning taurine with emphasis on the brain [J]. Adv Exp Med Biol, 2015, 803: 409-413. doi: 10.1007/978-3-319-15126-7_31.
[12] Jang H, Lee S, Choi SL, et al. Taurine directly binds to oligomeric amyloid-β and recovers cognitive deficits in Alzheimer model mice [J]. Adv Exp Med Biol, 2017, 975(Pt 1): 233-241. doi: 10.1007/978-94-024-1079-2_21.
[13] 李新娟,葛治国,李东亮,等.牛磺酸对大鼠脑损伤后认知功能及海马凋亡相关蛋白表达的影响[J]. 新乡医学院学报, 2006, 23(6): 569-571. LI Xinjuan, GE Zhiguo, LI Dongliang, et al. Effect of taurine on the cognitive function and the expression of protein related apoptosis in hippocampus after diffuse brain injury in rats [J]. Journal of Xinxiang Medical College, 2006, 23(6): 569-571.
[14] Wang K, Shi Y, Liu W, et al. Taurine improves neuron injuries and cognitive impairment in a mouse Parkinsons disease model through inhibition of microglial activation [J]. Neurotoxicology, 2021, 83: 129-136. doi: 10.1016/j.neuro.2021.01.002.
[15] Jakaria M, Azam S, Haque ME, et al. Taurine and its analogs in neurological disorders: Focus on therapeutic potential and molecular mechanisms [J]. Redox Biol, 2019, 24: 101223. doi: 10.1016/j.redox.2019.
[16] Xu Y, Wang S, Jiang L, et al. Identify melatonin as a novel therapeutic reagent in the treatment of 1-bromopropane(1-BP)intoxication [J]. Medicine(Baltimore), 2016, 95(3): e2203.
[17] Suo JN, Zhang CL, Wang P, et al. Allyl sulfide counteracts 1-bromopropane-induced neurotoxicity by inhibiting neuroinflammation and oxidative stress [J]. Toxicol Sci, 2019, 167(2): 397-407.
[18] Yang G, Xiang Y, Zhou W, et al. 1-Bromopropane-induced apoptosis in OVCAR-3 cells via oxidative stress and inactivation of Nrf2 [J]. Toxicol Ind Health, 2021, 37(2): 59-67.
[19] Castellanos E, Lanning NJ. Phosphorylation of OXPHOS machinery subunits: functional implications in cell biology and disease [J]. Yale J Biol Med, 2019, 92(3): 523-531.
[20] 徐建兴. 呼吸链的电子漏路径和线粒体的超氧自由基代谢及其生物学意义[J]. 基础医学与临床, 2001, 21(5): 389-394. XU Jianxing. Electronleak of respiratory chain and the metabolism of superoxide anion in mitochondria [J]. Basic Medical Sciences and Clinics, 2001, 21(5): 389-394.
[21] Cheng L, Chen L, Wei X, et al. NOD2 promotes dopaminergic degeneration regulated by NADPH oxidase 2 in 6-hydroxydopamine model of Parkinson's disease [J]. J Neuroinflammation, 2018, 15(1): 243.
[22] Han Z, Gao LY, Lin YH, et al. Neuroprotection of taurine against reactive oxygen species is associated with inhibiting NADPH oxidases [J]. Eur J Pharmacol, 2016, 777: 129-135. doi: 10.1016/j.ejphar.2016.03.006.
[23] Jong CJ, Azuma J, Schaffer S. Mechanism underlying the antioxidant activity of taurine: prevention of mitochondrial oxidant production [J]. Amino Acids, 2012, 42(6): 2223-2232.
[24] Baydas G, Reiter RJ, Akbulut M, et al. Melatonin inhibits neural apoptosis induced by homocysteine in Hippocampus of rats via inhibition of cytochrome c translocation and caspase-3 activation and by regulating pro- and anti-apoptotic protein levels [J]. Neuroscience, 2005, 135(3): 879-886.
[25] Yalçinkaya S, Unlüçerçi Y, Giri?瘙塂 M, et al. Oxidative and nitrosative stress and apoptosis in the liver of rats fed on high methionine diet: protective effect of taurine [J]. Nutrition, 2009, 25(4): 436-444.
[26] Leon R, Wu H, Jin Y, et al. Protective function of taurine in glutamate-induced apoptosis in cultured neurons [J]. J Neurosci Res, 2009, 87(5): 1185-1194.
[27] Taranukhin AG, Taranukhina EY, Saransaari P, et al. Neuroprotection by taurine in ethanol-induced apoptosis in the developing cerebellum J]. J Biomed Sci, 2010, 17(Suppl 1): S12.
[28] Niu XL, Zheng SM, Liu HT, et al. Protective effects of taurine against inflammation, apoptosis, and oxidative stress in brain injury [J]. Mol Med Rep, 2018, 18(5): 4516-4522.
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