Journal of Shandong University (Health Sciences) ›› 2018, Vol. 56 ›› Issue (1): 76-80.doi: 10.6040/j.issn.1671-7554.0.2017.580

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Effects of sympathetic signals on orthodontic root resorption in rats

WANG Qianqian1,3, WANG Xuxia2,3, QIAO Qingfang1,3, WANG Yingzi1,3, YANG Panpan1,3, ZHANG Jun1,3   

  1. 1. Department of Orthodontics, Stomatological Hospital of Shandong University;
    2. Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Shandong University;
    3. Key Lab of Oral Biomedicine of Shandong Province, Jinan 250012, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To explore the effects of sympathetic signals on root resorption in rats during orthodontic tooth movement. Methods Models of orthodontic tooth movement were established in 36 male 8-week old specific pathogen free(SPF)SD rats, which were randomly divided into 3 groups: propranolol(PRO)group, isoprenaline(ISO)group, and control group. The rats in the 3 groups were intraperitoneally injected with PRO［20 mg/(kg·d)］, ISO［5 mg/(kg·d)］ and isometric 0.9% saline, respectively, and were executed with force once every 7 days. After 14 days, the rats were sacrificed. The maxillae and first molars were separated. The root resorption was observed with hematoxylin-eosin(HE)staining.The volume of the mesial root of the first molars were measured with MicroCT. The differences among the 3 groups were compared using the SPSS 19.0 software. Results The results of HE staining showed that root resorption pits appeared in all 3 groups. The depth and scope of root resorption pits were the most serious in the ISO group, followed by the control and PRO groups. The results of MicroCT showed that the volume of mesial root of the first molars in the PRO group was larger than that in the control group(P<0.01), while the volume in the ISO group was obviously smaller than that in the control group(P<0.05). Conclusion Sympathetic signals can influence the root resorption during orthodontic tooth movement. The activation of signals promotes root resorption while the suppression of signals inhibits resorption.

Key words: Sympathetic nerve, Root resorption, MicroCT, Orthodontics, Rats

CLC Number: 

  • R783.5
[1] Rygh P. Ultrastructural changes in tension zones of rat molar periodontium incident to orthodontic tooth movement[J]. Am J Orthod, 1976, 70(3): 269-281.
[2] Owman-Moll P, Kurol J. Root resorption after orthodontic treatment in high- and low-risk patients: analysis of allergy as a possible predisposing factor[J]. Eur J Orthod, 2000, 22(6): 657-663.
[3] Lupi JE, Handelman CS, Sadowsky C. Prevalence and severity of apical root resorption and alveolar bone loss in orthodontically treated adults[J]. Am J Orthod Dentofacial Orthop, 1996, 109(1): 28-37.
[4] Brudvik P, Rygh P. The initial phase of orthodontic root resorption incident to local compression of the periodontal ligament[J]. Eur J Orthod, 1993, 15(4): 249-263.
[5] Weltman B, Vig KW, Fields HW, et al. Root resorption associated with orthodontic tooth movement: A systematic review[J]. Am J Orthod Dentofacial Orthop, 2010, 137(4): 462-476.
[6] Dahhas FY, El-Bialy T, Afify AR, et al. Effects of low-intensity pulsed ultrasound on orthodontic tooth movement and orthodontically induced inflammatory root resorption in ovariectomized osteoporotic rats[J]. Ultrasound in Med & Biol, 2016, 42(3): 808-814.
[7] 赵姝亚,王旭霞,刘宛鑫,等.帕米膦酸钠和伊班膦酸钠对正畸牙根吸收影响的动物实验[J]. 中华口腔医学杂志,2013,48(9):523-528. ZHAO Shuya, WANG Xuxia, LIU Wanxin, et al. Effect of pamidronate and ibandronate on orthodontic root resorption in rats[J]. Chin J Stomatol, 2013, 48(9): 523-528.
[8] Cherruau M, Morvan FO, Schirar A, et al. Chemical sympathectomy-induced changes in TH-,VIP-,and CGRP-immunoreactive fibers in the rat mandible periosteum: influence on bone resorption[J]. J Cell Physiol, 2003, 194(3): 341-348.
[9] Aitken SJ, Landao-Bassonga E, Ralston SH, et al. Beta2-adrenoreceptor ligands regulate osteoclast differentiation in vitro by direct and indirect mechanisms[J]. Arch Biochem Biophys, 2009, 482(1-2): 96-103.
[10] Vignaux G, Besnard S, Ndong J, et al. Bone remodeling is regulated by inner ear vestibular signals[J]. J Bone Miner Res, 2013, 28(10): 2136-2144.
[11] Takeda S, Elefteriou F, Levasseur R, et al. Leptin regulates bone formation via the sympathetic nervous system[J]. Cell, 2002, 111(3): 305-317.
[12] Elefteriou F, Ahn JD, Takeda S, et al. Leptin regulation of bone resorption by the sympathetic nervous system and CART[J]. Nature, 2005, 434(7032): 514-520.
[13] Kondo M, Kondo H, Miyazawa K, et al. Experimental tooth movement-induced osteoclast activation is regulated by sympathetic signaling[J]. Bone, 2013, 52(1): 39-47.
[14] 杨会芹,杨丽,陈君.焦虑症患者的心率变异性研究[J].中国行为医学科学,2016, 15(11): 1003-1004. YANG Huiqin, YANG Li, CHEN Jun. Analysis of heart rate variability in patients with anxiety[J]. Chin J of Behavioral Med Sci, 2016, 15(11): 1003-1004.
[15] Huang HH, Brennan TC, Muir MM, et al. Functional alpha1- and beta2-adrenergic receptors in human osteoblasts[J]. J Cell Physiol, 2009, 220(1): 267-275.
[16] Komoto S, Kondo H, Fukuta O, et al. Comparison of β-adrenergic and glucocorticoid signaling on clock gene and osteoblast-related gene expressions in human osteoblast[J]. Chronobiol Int, 2012, 29(1): 66-74.
[17] Aitken SJ, Landao-Bassonga E, Ralston SH, et al. Beta2-adrenoreceptor ligands regulate osteoclast differentiation in vitro by direct and indirect mechanisms[J]. Arch Bichem Biophys, 2009, 482(1/2): 96-103.
[18] Cao H, Kou X, Yang R, et al. Force-induced Adrb2 inperiodontal ligament cells promotes tooth movement[J]. J Dent Res, 2014, 93(11): 1163-1169.
[19] Sasaki T. Differentiation and functions of osteoclasts andodontoclasts in mineralized tissue resorption[J]. Microsc Res Tech, 2003, 61(6): 483-495.
[20] 卢嘉静,葛振林.正畸致牙根吸收的分子生物学研究进展[J]. 国际口腔医学杂志, 2008, 35(5): 599-601. LU Jiajing, GE Zhenlin. Research process of the molecular mechanism of dental root resorption induced by orthodontic treatment[J]. Int J Stomatol, 2008, 35(5): 599-601.
[21] Hellsing E, Hammarstrom L. The hyaline zone andassociated root surface changes in experimental orthodonticsin rats: a light and scanning electron microscope study[J]. Eur J Orthod, 1996, 18(1): 11-18.
[22] Wierzbicki T, Ei-Bialy T, Aldaghreer S, et al. Analysis of orthodonticallyinducedrootresorption using micro-computed tomography(Micro-CT)[J]. Angle Orthod, 2009, 79(1): 91-96.
[23] 王慧媛,胡睿,艾林,等. Micro-CT应用于口腔治疗的初步研究[J].中国美容医学, 2012, 21(5): 823-825. WANG Huiyuan, HU Rui, AI Lin, et al. Primary research of using Micro-CT in oral therapy[J]. Chinese Journal of Aesthetic Medicine, 2012, 21(5): 823-825.
[24] 庄丽,孟宪莹,李萍,等.正畸牙根吸收过程中牙根三维形态的MICRO-CT初步研究[J].现代口腔医学杂志, 2010, 24(1): 35-38. ZHUANG Li, MENG Xianying, LI Ping, et al. A pilot study on three dimensional morphology of root by Micro-CT during orthodontic root resorption[J]. J Modern Stomatol, 2010, 24(1): 35-38.
[25] Li J, Wang X, Li N, et al. Short-term effects of nicotine on orthodontically induced root resorptionin rats[J]. Angle Orthod, 2016, 86(2): 199-205.
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