Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (5): 98-106.doi: 10.6040/j.issn.1671-7554.0.2019.1394

Previous Articles    

Effects of high-sugar, high-fat diet and different concentrations of selenium on lipid metabolism and oxidative stress in rats

LI Yan, SUN Fengjiao, ZHANG Tianran, WANG Yuxin, ZHANG Zhengduo, GAO Xibao   

  1. School of Public Health, Shandong University, Jinan 250012, Shandong, China
  • Published:2022-09-27

PDF (PC)

38

Abstract: Objective To observe the effects of high-sugar and high-fat diet on lipid metabolism and oxidative stress in rats and the effects of different selenium concentrations in different diets. Methods Sodium selenite was used to adjust the selenium concentration in the feed, and 90 rats were randomly divided into 9 groups and fed for 16 weeks. The body weight was measured, organ coefficient was calculated, and serum triglycerides(TG), total cholesterol(TC), concentrations of lipase and selenium were measured. After preparation of liver and heart homogenates, glutathione peroxidase(GPx), malondialdehyde(MDA), total superoxide dismutase(T-SOD), and copper-zinc superoxide dismutase(CuZu-SOD)were measured. Pathological changes of liver and heart were observed. Results High-sugar rats had higher TG and MDA, and high-fat rats had higher TC and MDA than normal-diet rats(P<0.05). Normal-medium-selenium rats had lower serum TG, and normal-high-selenium rats had lower cardiac GPx than normal-low-selenium rats(P<0.05). High-sugar-medium-selenium rats had higher hepatic GPx but lower cardiac MDA than high-sugar-low-selenium rats(P<0.05). High-fat-high-selenium rats had higher serum TG and cardiac MDA than high-fat-medium-selenium rats and high-fat-low-selenium rats(P<0.05). High-fat-medium-selenium rats had lower hepatic MDA than high-fat-low-selenium rats(P<0.05). High-glucose-high-selenium rats and high-fat-high-selenium rats had increased serum selenium(P<0.05). Conclusion Appropriate selenium supplementation has a protective effect on lipid metabolism and antioxidation in rats. Gender may affect the effect of high concentrations of selenium on high-glucose and high-fat rats. The mechanism remains to be explored.

Key words: Dietary pattern, High-sugar diet, High-fat diet, Selenium, Lipid metabolism, Oxidative stress

CLC Number: 

  • R15
[1] Schomburg L. Dietary Selenium and Human Health [J]. Nutrients, 2017, 9(1): 22.
[2] Wu G, Li Z, Ju W, et al. Cross-sectional study: relationship between serum selenium and hypertension in the Shandong Province of China [J]. Biol Trace Elem Res, 2018, 185(2): 295-301.
[3] Hasani M, Djalalinia S, Sharifi F, et al. Effect of selenium supplementation on lipid profile: a systematic review and meta-analysis [J]. Horm Metab Res, 2018, 50(10): 715-727.
[4] Ju W, Li X, Li Z, et al. The effect of selenium supplementation on coronary heart disease: A systematic review and meta-analysis of randomized controlled trials [J]. J Trace Elem Med Biol, 2017, 44: 8-16. doi: 10.1016/j.jtemb.2017.04.009.
[5] Semiane N, Foufelle F, Ferré P, et al. High carbohydrate diet induces nonalcoholic steato-hepatitis(NASH)in a desert gerbil[J]. C R Biol, 2017, 340(1): 25-36.
[6] Mendes IKS, Matsuura C, Aguila MB, et al. Weight loss enhances hepatic antioxidant status in a NAFLD model induced by high-fat diet [J]. Appl Physiol Nutr Metab, 2018, 43(1): 23-29.
[7] Burgeiro A, Cerqueira MG, Varela-Rodriguez BM, et al. Glucose and lipid dysmetabolism in a rat model of prediabetes induced by a high-sucrose diet [J]. Nutrients, 2017, 9(6): 638.
[8] Kratz M, Baars T, Guyenet S. The relationship between high-fat dairy consumption and obesity, cardiovascular, and metabolic disease [J]. Eur J Nutr, 2013, 52(1): 1-24.
[9] Han Q, Yeung SC, Ip MSM. Dysregulation of cardiac lipid parameters in high-fat high-cholesterol diet-induced rat model [J]. Lipids Health Dis, 2018, 17(1): 255.
[10] Dong HL, Yuan N, Sun T, et al. Effects of selenium supplement on atherogenesis of ApoE-knockout mice fed high fat diet[J]. China Medical Abstracts, 2016, 44(2): 244.
[11] Farrokhian A, Bahmani F, Taghizadeh M, et al. Selenium supplementation affects insulin resistance and serum hs-CRP in patients with type 2 diabetes and coronary heart disease [J]. Horm Metab Res, 2016, 48(4): 263-268.
[12] Alehagen U, Aaseth J, Alexander J, et al. Still reduced cardiovascular mortality 12 years after supplementation with selenium and coenzyme Q10 for four years: A validation of previous 10-year follow-up results of a prospective randomized double-blind placebo-controlled trial in elderly [J]. PLoS One, 2018, 13(4): 0193120. doi: 10.1371/journal.pone.0193120.
[13] Wang N, Tan HY, Li S, et al. Supplementation of micronutrient selenium in metabolic diseases: its role as an antioxidant [J]. Oxid Med Cell Longev, 2017, 2017: 7478523. doi: 10.1155/2017/7478523.
[14] 时盼盼, 王芙蓉. 微量元素硒与高血压、冠心病相关性研究进展 [J]. 社区医学杂志, 2018, 16(1): 77-79.
[15] Afshin A, Sur PJ, Fay KA, et al. Health effects of dietary risks in 195 countries, 1990-2017: a systematic analysis for the Global Burden of Disease Study 2017 [J]. Lancet, 2019, 393(10184): 1958-1972.
[16] Hu X, Chandler JD, Orr ML, et al. Selenium supplementation alters hepatic energy and fatty acid metabolism in mice [J]. J Nutr, 2018, 148(5): 675-684.
[17] Qian Z, Qian ZY, Zhou HP, et al. Effects of oral selenium and magnesium co-supplementation on lipid metabolism, antioxidative status, histopathological lesions, and related gene expression in rats fed a high-fat diet [J]. Lipids Health Dis, 2018, 17(1): 165.
[18] 满青青, 何丽. 高糖高脂饲料对Wistar大鼠生长和糖脂代谢的影响[J]. 卫生研究, 2009, 38(6): 722-724. MAN Qingqing, HE Li. Effects of high-sugar and high-fat diet on growth and carbohydrate, lipid metabolism in Wistar rats [J]. Journal of Hygiene Research, 2009, 38(6): 722-724.
[19] Chalkley SM, Hettiarachchi M, Chisholm DJ, et al. Long-term high-fat feeding leads to severe insulin resistance but not diabetes in Wistar rats [J]. Am J Physiol Endocrinol Metab, 2002, 282(6): 1231-1238.
[20] 苏琳, 刘玉兰. 高糖饮食及高脂饮食建立非酒精性脂肪肝大鼠模型的比较[J]. 实验动物科学, 2009, 26(3): 14-17.
[21] Ozay R, Uzar E, Aktas A, et al. The role of oxidative stress and inflammatory response in high-fat diet induced peripheral neuropathy [J]. J Chem Neuroanat, 2014, 55: 51-57. doi: 10.1016/j.jchemneu.2013.12.003.
[22] Farhangi MA, Nameni G, Hajiluian G, et al. Cardiac tissue oxidative stress and inflammation after vitamin D administrations in high fat-diet induced obese rats [J]. BMC Cardiovasc Disord, 2017, 17(1): 161.
[23] 梁冰, 王舒然. 高脂饮食诱导肥胖导致线粒体损伤的研究现状[J]. 中华预防医学杂志, 2013, 47(4): 371-374.
[24] 田园, 牟维鹏, 李鹏高, 等. 补充不同水平亚硒酸钠和硒蛋氨酸对大鼠硒代谢的影响[J]. 卫生研究, 2004, 33(6): 749-751.
[25] 计峰, 苏琪, 赵学志, 等. 不同剂量补硒对大鼠氧化应激损伤的保护作用[J]. 营养学报, 2019, 41(1): 63-67. JI Feng, SU Qi, ZHAO Xuezhi, et al. Protective effect on oxidative stress injury by selenium supplementation in rats[J]. Acta Nutrimenta Sinica, 2019, 41(1): 63-67.
[26] Friedman SL, Neuschwander-Tetri BA, Rinella M, et al. Mechanisms of NAFLD development and therapeutic strategies[J]. Nat Med, 2018, 24(7): 908-922.
[1] WU Hong, ZHANG Zhengduo, TANG Yanjin, QI Shaojun, GAO Xibao. Potential intervention effects of 5-methyltetrahydrofolate on atherosclerosis in rats [J]. Journal of Shandong University (Health Sciences), 2022, 60(8): 6-13.
[2] LIU Min, ZHANG Yuchao, MA Xiaoli, LIU Xinyu, SUN Lu, ZUO Dan, LIU Yuantao. Effects of orphan nuclear receptor NR4A1 on H2O2 induced injury of mouse renal podocytes [J]. Journal of Shandong University (Health Sciences), 2022, 60(5): 16-21.
[3] HU Na, SUN Miao, XING Shasha, XU Danxia, HAI Xiaoming, MA Ling, YANG Li, MIAN Yuchen, HE Rui, CHEN Dongmei, MA Huiming. Evening primrose oil resists oxidative stress in the ovaries of rats with polycystic ovary syndrome [J]. Journal of Shandong University (Health Sciences), 2022, 60(5): 22-30.
[4] HUANG Huining, DU Juanjuan, SUN Yi, HOU Yinglong, GAO Mei. Hydrogen sulfide alleviates acute obstructive sleep apnea-induced atrial fibrillation by regulating oxidative stress through glutaredoxin-1 [J]. Journal of Shandong University (Health Sciences), 2022, 60(1): 1-5.
[5] Minqi LI,Juan DU,Panpan YANG,Yuying KOU,Shanshan LIU. Research progress of oxidative stress regulating osteoporosis [J]. Journal of Shandong University (Health Sciences), 2021, 59(6): 16-24.
[6] LIU Shudan, ZHANG Feiyan, GUO Songlin, LIANG Xueyun, CHEN Dongmei. Oxymatrine ameliorates oxidative stress injury of HaCaT cells induced by hypoxia ischemia [J]. Journal of Shandong University (Health Sciences), 2021, 59(3): 26-34.
[7] XIANG Lin, CHEN Lamei, WANG Jingwen, LI Haiming, LI Haoyu, WANG Ju, FAN Yuchen, WANG Kai. Effects of drinking Yin-Jiu-Shu on liver metabolizing enzymes in 30 healthy volunteers [J]. Journal of Shandong University (Health Sciences), 2021, 59(3): 81-85.
[8] ZHANG Jijuan, YU Hancheng, WANG Lan, CHEN Nuo, CUI Shumeng, Gao Xibao. Effects of high-fat diet and selenium concentration on antioxidant function in rats [J]. Journal of Shandong University (Health Sciences), 2021, 59(1): 95-101.
[9] SUN Fengjiao, LI Yan, WANG Yuxin, ZHANG Tianran, WU Hong, GAO Xibao. Effects of dietary structure and selenium concentration on glucose metabolism in rats [J]. Journal of Shandong University (Health Sciences), 2020, 58(2): 36-43.
[10] LYU Yan, YU Xiao, LIN Xinying, ZHAO Qi, WANG Baozhen. Association between dietary patterns and depressive symptoms among Chinese elderly women [J]. Journal of Shandong University (Health Sciences), 2020, 58(11): 103-108.
[11] LIU Chongdong, LOU Tong, DONG Jing. Malignant transformation of endometriosis [J]. Journal of Shandong University (Health Sciences), 2019, 57(6): 27-32.
[12] JIANG Lijuan, LIU Fuqiang, JIANG Ziyun, LI Wenjuan, LIN Peng, WANG Chuan, HOU Xinguo, CHEN Li. Dapagliflozin improves lipid metabolism and visceral adipose tissues in overweight and obese patients with type 2 diabetes [J]. Journal of Shandong University (Health Sciences), 2019, 57(6): 87-93.
[13] DU Hao, CHENG Yugang, HUANG Xin, LIU Shaozhuang, ZHANG Guangyong, HU Sanyuan. Effects of sleeve gastrectomy on lung injury in type 2 diabetic rats [J]. Journal of Shandong University (Health Sciences), 2019, 57(4): 20-26.
[14] ZHANG Xiaotao, HE Tianqi, ZHU Meijia, TANG Jiyou, ZHAO Zhangning, MAO Fei, FANG Yuqing, LIU Xiaomin, MA Gaoting, ZHANG Xiaoyu, ZHANG Xiao, WANG Min, LI Xiuhua. Clinical observation of the effects of idebenone on Parkinsons disease [J]. Journal of Shandong University (Health Sciences), 2019, 57(4): 34-41.
[15] ZHANG Xiuling, ZHANG Lei, LIU Dan, LIANG Jiangjiu. Association of ApoE gene polymorphism and risk of atrial fibrillation among Han people in Shandong Province [J]. Journal of Shandong University (Health Sciences), 2018, 56(3): 79-84.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd