Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (5): 98-106.doi: 10.6040/j.issn.1671-7554.0.2019.1394

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Effects of high-sugar, high-fat diet and different concentrations of selenium on lipid metabolism and oxidative stress in rats

LI Yan, SUN Fengjiao, ZHANG Tianran, WANG Yuxin, ZHANG Zhengduo, GAO Xibao   

  1. School of Public Health, Shandong University, Jinan 250012, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To observe the effects of high-sugar and high-fat diet on lipid metabolism and oxidative stress in rats and the effects of different selenium concentrations in different diets. Methods Sodium selenite was used to adjust the selenium concentration in the feed, and 90 rats were randomly divided into 9 groups and fed for 16 weeks. The body weight was measured, organ coefficient was calculated, and serum triglycerides(TG), total cholesterol(TC), concentrations of lipase and selenium were measured. After preparation of liver and heart homogenates, glutathione peroxidase(GPx), malondialdehyde(MDA), total superoxide dismutase(T-SOD), and copper-zinc superoxide dismutase(CuZu-SOD)were measured. Pathological changes of liver and heart were observed. Results High-sugar rats had higher TG and MDA, and high-fat rats had higher TC and MDA than normal-diet rats(P<0.05). Normal-medium-selenium rats had lower serum TG, and normal-high-selenium rats had lower cardiac GPx than normal-low-selenium rats(P<0.05). High-sugar-medium-selenium rats had higher hepatic GPx but lower cardiac MDA than high-sugar-low-selenium rats(P<0.05). High-fat-high-selenium rats had higher serum TG and cardiac MDA than high-fat-medium-selenium rats and high-fat-low-selenium rats(P<0.05). High-fat-medium-selenium rats had lower hepatic MDA than high-fat-low-selenium rats(P<0.05). High-glucose-high-selenium rats and high-fat-high-selenium rats had increased serum selenium(P<0.05). Conclusion Appropriate selenium supplementation has a protective effect on lipid metabolism and antioxidation in rats. Gender may affect the effect of high concentrations of selenium on high-glucose and high-fat rats. The mechanism remains to be explored.

Key words: Dietary pattern, High-sugar diet, High-fat diet, Selenium, Lipid metabolism, Oxidative stress

CLC Number: 

  • R15
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