JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2016, Vol. 54 ›› Issue (7): 1-5.doi: 10.6040/j.issn.1671-7554.0.2015.1340

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Brain-derived neurotrophic factor modulates intestinal barrier integrity in mice

ZHAO Dongyan, YU Yanbo, ZUO Xiuli   

  1. Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2015-12-25 Online:2016-07-10 Published:2016-07-10

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Abstract: Objective To investigate the effects of brain-derived neurotrophic factor(BDNF)on intestinal barrier integrity in mice. Methods Ileal tissues and colonic tissues from BDNF+/+ mice and BDNF+/- mice were prepared. The intestinal epithelial structure was detected by transmission electron microscopy. Expressions and distributions of tight junction proteins(occludin and ZO-1)in the colonic mucosa were evaluated by immunohistochemistry. Expressions of tight junction proteins(claudin-1 and claudin-2)in the colonic mucosa were evaluated by Western blotting. Results Compared with BDNF+/+ mice, BDNF+/- mice displayed integral ileal epithelial barrier and impaired colonic mucosa integrity which was characterized by diminished microvilli, widened intercellular space and bacteria invasion in the colonic mucosa. BDNF+/- mice also exhibited increased expression of claudin-2 and decreased expression of occludin, ZO-1 and claudin-1 in the colonic mucosa. Conclusion BDNF may play a role in regulating the colonic epithelial barrier in mice.

Key words: Brain-derived neurotrophic factor, Intestinal barrier, Mice, Tight junction proteins

CLC Number: 

  • R574
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