Journal of Shandong University (Health Sciences) ›› 2017, Vol. 55 ›› Issue (12): 18-23.doi: 10.6040/j.issn.1671-7554.0.2017.658

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Effect of myosin light chain kinase on airway inflammation and lung function in asthma model mice

GONG Xiaodan1,2, ZHAO Fangzheng2,3, CAO Ke1,2, DENG Penghui2, ZHANG Caiqing2   

  1. 1. Medical College of Shandong University, Jinan 250012, Shandong, China;
    2. Respiratory Physicians of Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, Shandong, China;
    3. The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250001, Shandong, China
  • Online:2017-12-20 Published:2022-09-27

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Abstract: Objective To study the effect of myosin light chain kinase(MLCK)on airway inflammation and pulmonary function through the determination of its expression in asthmatic mice airway. Methods Twenty female BALB/c mice were randomly divided into normal control group(n=10)and asthma group(n=10). Mice model of asthma was established by using chicken ovalbumin(OVA). The counts of leukocytes and eosinophilic granulocytes were observed with optical microscope. The expressions of the inflammatory mediators as IL-4 and IFN-γ in bronchoalveolar lavage fluid(BALF)were detected by ELISA kit. The expression of MLCK in the lung tissue of asthmatic mice was detected by Western blotting. The expression of MLCK in the gene level was detected by qPCR. Results The lung function test showed that peak expiratory flow(PEF), the ratio of forced expiratory volume in 0.4 second to forced vital capacity(FEV0.4/FVC)in asthma group was lower than those in normal control group(P<0.001). The counts of leukocytes and eosinophilic granulocytes in asthma group were higher than those in normal control group(P<0.001). The IL-4 level in the BALF in asthma group was higher than that in normal control group(P<0.001), while the IFN-γ level 山 东 大 学 学 报 (医 学 版)55卷12期 -宫晓丹,等.肌球蛋白轻链激酶对哮喘小鼠气道炎症及肺功能的影响 \=-was lower than that in nornal control group(P<0.001). The expression of MLCK in the lung tissue of asthma group was significantly increased(P=0.006). The expression of MLCK in the gene level of asthma group was higher compared with the normal control group(P<0.001). Conclusion MLCK may be involved in the airway inflammation response and influence the lung function.

Key words: Asthma, Myosin light chain kinase, Lung function, IL-4, IFN-γ

CLC Number: 

  • R562.2+5
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