Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (1): 55-61.doi: 10.6040/j.issn.1671-7554.0.2018.1089

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Anti-inflammatory mechanism of ephedra treatment of asthma based on network pharmacology

CHEN Ou1,2, LI Guoyong3, LIU Aihong2, ZHU Xiaobo2, CHEN Shaojie2, WANG Yibiao2   

  1. 1. Community Room, School of Nursing, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Pediatrics, The Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    3. Certification Review Center of Shandong Food and Drug Administration, Jinan 250014, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To explore the pathogenesis of asthma by predicting the anti-inflammatory targets and related signaling pathway of ephedra therapy based on network pharmacology. Methods The active ingredients of ephedra were searched and screened in TCMSP database. The targets of ingredients were predicted with PharmMapper and molecular docking was performed. Then ingredients-targets network was established and analyzed with cytoscape3.6.1. The anti-inflammatory targets in TTD database were searched to build PPI network, which was merged with the ingredients-targets network to screen anti-inflammatory targets connected with ephedra. The vivo reaction network of ephedra anti-inflammatory targets against asthma was constructed to screen anti-inflammatory targets related to asthma. KEGG enrichment analysis was performed with Enrichr database and cytoscape3.6.1. Results Altogether 23 active ingredients were screened and 156 targets were obtained. Epidermal active growth factor receptor(EGFR), E-selectin(SELE), macrophage migration inhibitory factor(MIF), mitogen-activated protein kinase 14(MAPK14)might be the important anti- 山 东 大 学 学 报 (医 学 版)57卷1期 -陈欧,等.网络药理学预测麻黄治疗哮喘的抗炎作用机制 \=-inflammatory targets of ephedra treatment of asthma. All these pathways had epithelial cell signaling pathways. Conclusion The anti-inflammatory mechanism of ephedra treatment of asthma may be related to EGFR, SELE, MIF, MAPK14 and their signaling pathways. To prevent the exacerbation of asthma, instead of a single pathway or a single target, all these targets and their signaling pathways should be controlled holistically.

Key words: Asthma, Ephedra, Network pharmacology, Anti-inflammatory, Target prediction, Pathway

CLC Number: 

  • R725.4
[1] Olin JT, Wechsler ME. Asthma: pathogenesis and novel drugs for treatment[J]. BMJ, 2014, 24, 349: g5517. doi: 10.1136/bmj.g5517.
[2] Drake MG, Scott GD, Blum ED, et al. Eosinophils increase airway sensory nerve density in mice and in human asthma[J]. Sci Trans Med, 2018, 10(457), pii: eaar8477. doi: 10.1126/scitranslmed.aar8477.
[3] 杨昕宇, 肖长芳, 张凯熠, 等. 麻黄临床应用与药理作用研究进展[J]. 中华中医药学刊, 2015, 33(12): 2874-2877. YANG Xiyu, XIAO Changfang, ZHANG Kaiyi, et al. Research Progress on Clinical Application and Pharmacological Functions of Ephedra[J]. Chinese archives of traditional Chinese medicine, 2015, 33(12): 2874-2877.
[4] Hasan S, Bonde BK, Buchan NS, et al. Network analysis has diverse roles in drug discovery[J]. Drug Discov Today, 2012, 17(15-16): 869-874.
[5] Zhai L, Ning ZW, Huang T, et al. Cyclocarya paliurus Leaves Tea Improves Dyslipidemia in Diabetic Mice: A Lipidomics-Based Network Pharmacology Study[J]. Front Pharmacol, 2018, 9: 973. doi: 10.3389/fphar.2018.00973.
[6] 汝锦龙. 中药系统药理学数据库和分析平台的构建和应用[D].咸阳: 西北农林科技大学, 2015.
[7] Xu X, Zhang WX, Huang C, et al. A novel chemometric method for the prediction of human oral bioavailability[J]. Int J Mol Sci, 2012, 13(6): 6964-6982.
[8] Yamanishi Y, Kotera M, Kanehisa M, et al. Drug-target interaction prediction from chemical, genomic and pharmacological data in an integrated framework[J]. Bioinformatics, 2010, 26(12): i246-i254. doi: 10.1093/bioinformatics/btq176.
[9] 刘学. 基于网络药理学方法研究玉屏风散治疗哮喘的作用机制[D]. 成都: 西南交通大学, 2017.
[10] Bi YH, Zhang LH, Chen SJ, et al. Antitumor Mechanisms of Curcumae Rhizoma Based on Network Pharmacology[J]. Evid Based Complement Alternat Med, 2018, 2018: 4509892. doi: 10.1155/2018/4509892.
[11] Kun-Yi H, Yukiko M, Yoshiyuki A, et al. Systemsdock: a web server of network pharmacology-based prediction and analysis[J]. Nucleic Acids Res, 2016, 44(W1): W507-W513. doi: 10.1093/nar/gkw335.
[12] Hsin KY, Ghosh S, Kitano H. Combining machine learning systems and multiple docking simulation packages to improve docking prediction reliability for network pharmacology[J]. PLoS One, 2013, 8(12): e83922. doi:10.1371/journal.pone.0083922.
[13] Szklarczyk D, Morris JH, Cook H, et al. The STRING database in 2017: quality-controlled protein-protein association networks, made broadly accessible[J]. Nucleic Acids Res, 2017, 45(D1): D362-D368. doi: 10.1093/nar/gkw937.
[14] 雷奇林, 黄雅兰, 钟茜, 等. 基于网络药理学的黄芩抗炎作用机制研究[J]. 中草药, 2018, 49(15): 3523-3530. LEI Qilin, HUANG Yalan, ZHONG Qian, et al. Anti-inflammatory mechanism of Scutellarlae Radix based on network pharmacology[J]. Chinese Traditional and Herbal Drugs, 2018, 49(15): 3523-3530.
[15] Casale TB, Pacou M, Mesana L, et al. Reslizumab Compared with Benralizumab in Patients with Eosinophilic Asthma: A Systematic Literature Review and Network Meta-Analysis[J]. J Allergy Clin Immunol Pract, 2018. pii: S2213-2198(18)30576-2. doi: 10.1016/j.jaip.2018.08.036
[16] Hekking PP, Bel EH. Developing and emerging clinical asthma phenotypes[J]. J Allergy Clin Immunol Pract, 2014, 2(6): 671-680.
[17] Chen SJ, Cui MC. Systematic Understanding of the Mechanism of Salvianolic Acid A via Computational Target Fishing[J]. Molecules, 2017, 22(4). pii: E644. doi: 10.3390/molecules22040644.
[18] 朱婉婷, 范雪梅, 位华, 等. 基于网络药理学探讨阿帕替尼治疗乳腺癌的作用制剂[J]. 中国药学杂志, 2016, 51(18): 1569-1573. ZHU Wanting, FAN Xuemei, WEI Hua, et al. Mechanism Research of Apatinib-treated Breast Cancer Based on Network Pharmacology[J]. Chinese Pharmaceutical Journal, 2016, 51(18): 1569-1573.
[19] Sokolova EA, Vodeneev VA, Deyev SM, et al. 3D in vitro models of tumors expressing EGFR family receptors: a potent tool for studying receptor biology and targeted drug development[J]. Drug Discov today, 2018, pii: S1359-6446(18)30168-5. doi: 10.1016/j.drudis.2018.09.003.
[20] Vinod Prabhu V, Elangovan P, Niranjali Devaraj S, et al. Targeting apoptosis by 1, 2-diazole through regulation of EGFR, Bcl-2 and CDK-2 mediated signaling pathway in human non-small cell lung carcinoma A549 cells[J]. Gene, 2018, 679: 352-359. doi:10.1016/j.gene.2018.09.014.
[21] 晋乐飞, 吴卫东, 段丽菊, 等. 表皮生长因子受体在臭氧致小鼠肺部炎症中的作用[J]. 郑州大学学报(医学版), 2016, 51(4): 450-454. JIN Yuefei, WU Weidong, DUAN Liju, et al. Role of EGF receptor in ozone-induced lung inflammation in mice[J]. Journal of Zhengzhou University(Medical Sciences), 2016, 51(4): 450-454.
[22] Yoshikawa T, Kanazawa H. Integrated effect of EGFR and PAR-1 signaling crosstalk on airway hyperresponsiveness[J]. Int J Mol Med, 2012, 30(1): 41-48.
[23] Habibovic A, Hristova M, Heppner DE, et al. DUOX1 mediates persistent epithelial EGFR activation, mucous cell metaplasia, and airway remodeling during allergic asthma[J]. JCI Insight, 2016, 1(18): e88811. doi:10.1172/jci.insight.88811
[24] Wang X, Yang XQ, Li Y, et al. Lyn kinase represses mucus hypersecretion by regulating IL-13-induced endoplasmic reticulum stress in asthma[J]. EBioMedicine, 2017, 15: 137-149. doi:10.1016/j.ebiom.2016.12.010.
[25] Tsoref O, Tyomkin D, Amit U, et al. E-selectin-targeted copolymer reduces atherosclerotic lesions, adverse cardiac remodeling, and dysfunction[J]. J Control Release, 2018, 288: 136-147. doi: 10.1016/j.jconrel.2018.08.029.
[26] Hamzaoui A, Ammar J, El Mekki F, et al. Elevation of serum soluble E-selectin and VCAM-1 in severe asthma[J]. Mediators Inflamm, 2001, 10(6): 339-342.
[27] Bijanzadeh M, Ramachandra NB, Mahesh PA, et al. Soluble intercellular adhesion molecule-1 and E-selectin in patients with asthma exacerbation[J]. Lung, 2009, 187(5): 315-320.
[28] Sabroe I, Pease JE, Williams TJ. Asthma and MIF: innately Th1 and Th2[J]. Clin Exp Allergy, 2000, 30(9): 1194-1196.
[29] Yang M, Kumar RK, Hansbro PM, et al. Emerging roles of pulmonary macrophages in driving the development of severe asthma[J]. J Leukoc Biol, 2012, 91(4): 557-569.
[30] Wu J, Fu S, Ren X, et al. Association of MIF promoter polymorphisms with childhood asthma in a northeastern Chinese population[J]. Tissue Antigens, 2009, 73(4): 302-306.
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