Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (7): 66-73.doi: 10.6040/j.issn.1671-7554.0.2022.0183

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Effects of Sdccag3 on the osseointegration in hyperlipidemia rats through Wnt pathway

YUAN Mengqi, HUO Fenglei, REN Huiping, GUO Qiushuang, LAN Jing   

  1. Department of Prosthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University &
    Shandong Key Laboratory of Oral Tissue Regeneration &
    Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan 250012, Shandong, China
  • Published:2022-07-27

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Abstract: Objective To investigate the effects of serologically defined colon cancer antigen-3(Sdccag3)on the differentiation of bone marrow mesenchymal stem cells(BMSCs)and the osteointegration around implants in hyperlipidemia rats through Wnt signaling pathway in high lipid environment. Methods BMSCs were cultured in hyperlipidemia rat models and induced by high lipid environment. Sdccag3 expression was overexpressed/silenced by lentivirus vector. RT-PCR, Western blotting, Micro-CT, HE staining, Oil red O staining and immunofluorescence staining were used to assess the effects of high lipid environment and Sdccag3 on bone metabolism and BMSCs differentiation balance. Results Sdccag3 was overexpressed in BMSCs induced by high lipid osteogenesis, which inhibited lipid differentiation indexes peroxisome proliferators-activated receptors γ(FABP4)and peroxisome proliferators-activated receptors γ(PPAR-γ), up-regulated Wnt signaling pathway marker genes low density lipoprotein receptor-related protein 5(LRP5), low density lipoprotein receptor-related protein 6(LRP6), β-catenin, Wnt family member 5a(Wnt5a)and Wnt family member 5b(Wnt5b), and promoted osteogenic differentiation indexes alkaline phosphatase(ALP)and runt-related transcription factor 2(Runx2). In hyperlipidemia rats, overexpression of Sdccag3 up-regulated Wnt signaling pathway marker genes and inhibited peripheral lipid formation, while silencing LRP5 down-regulated Sdccag3, inhibited osteogenic differentiation and promoted lipid differentiation, which were consistent with silencing Sdccag3 expression. Conclusion Hyperlipidemia inhibits Sdccag3 expression. Overexpression of Sdccag3 inhibits adipogenic differentiation of BMSCs and decreases lipid formation around the implants in hyperlipidemia rats, promotes osteogenesis and expression of Wnt signaling pathway marker genes.

Key words: Hyperlipidemia, Sdccag3, Adipogenesis, Wnt pathway, Low density lipoprotein receptor-related protein 5

CLC Number: 

  • R783
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