Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (2): 29-35.doi: 10.6040/j.issn.1671-7554.0.2019.830

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Early expression of Frizzled 4 in peri-implant tissues of hyperlipidemic rats

LI Yuan1, WANG Qiang2, SHI Xiaolei2, ZHANG Xiaoli2, XU Xin1   

  1. 1. Department of Implantology, School and Hospital of Stomatology, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Stomatology, Weifang Yidu Central Hospital, Qingzhou 262500, Shandong, China
  • Online:2020-02-10 Published:2022-09-27

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Abstract: Objective To investigate the early expression of Frizzled 4 in peri-implant tissues of hyperlipidemic rats. Methods A total of 48 adult male SD rats were randomly divided into two groups, with 24 rats in either group. Rats in the experimental group were fed with high fat diet, while rats in the control group were fed with common diet for 8 weeks. Implants 1.5 mm wide and 2.5 mm long were implanted into the metaphyseal end of femur. The rats were sacrificed on the 1st, 3rd, 5th and 28th days after operation to obtain bone tissues around the implant. The tissues were scanned with Inveon MM CT and 1 mm area around the implant was set as the area of interest(ROI). Three-dimensional reconstruction was performed in ROI. Bone trabecular parameters were analyzed, including trabecular volume/ROI volume(BV/TV), trabecular thickness(Tb.Th), trabecular number(Tb.N)and trabecular space(Tb.S). The gene expressions of osteoblasts, osteoclast differentiation markers Runt-related transcription factor 2(RUNX2), cathepsin K(CTSK)and Frizzled 4 were detected with RT-PCR. The protein expression of Frizzled 4 was determined with Western 山 东 大 学 学 报 (医 学 版)58卷2期 -李源,等.卷曲受体蛋白4在高脂血症大鼠种植体周围组织中的早期表达 \=- blotting. Results There were no significant differences in BV/TV, Tb.S, Tb.Th and Tb.N between the two groups on the 1st and 3rd day of implantation(P>0.05). On the 5th and 28th days of implantation, the experimental group had lower BV/TV, Tb.Th and Tb.N but higher Tb.S than the control group(P<0.05). On the 1st, 3rd, 5th and 28th days of implantation, the experimental group had lower mRNA expression of Runx2 but higher mRNA expression of CTSK than the control group(P<0.05). In the control group, the mRNA and protein expressions of Frizzled 4 increased significantly on the 3rd day of implantation, and decreased on the 5th and 28th days, but were still higher than those in the experimental group(P<0.05). In the experimental group, both mRNA and protein expressions of Frizzled 4 decreased significantly on the 3rd day of implantation, but increased on the 5th and 28th days(P<0.05). In the control group, the protein expression of Wnt/β-catenin increased significantly on the 3rd day of implantation, and decreased on the 5th and 28th days, but was still higher than that in the experimental group(P<0.05). In the experimental group, the expression of Wnt/β-catenin increased significantly on the 3rd day of implantation, but decreased on the 5th and 28th days(P<0.05). Conclusion Hyperlipidemia can inhibit early osseointegration of implants, which may be related to the down-regulation of Frizzled 4 expression. The exact mechanism needs further research.

Key words: Frizzled 4, Hyperlipidemia, Implants, Osteointegration

CLC Number: 

  • R782
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