Journal of Shandong University (Health Sciences) ›› 2020, Vol. 58 ›› Issue (2): 29-35.doi: 10.6040/j.issn.1671-7554.0.2019.830

Previous Articles     Next Articles

Early expression of Frizzled 4 in peri-implant tissues of hyperlipidemic rats

LI Yuan1, WANG Qiang2, SHI Xiaolei2, ZHANG Xiaoli2, XU Xin1   

  1. 1. Department of Implantology, School and Hospital of Stomatology, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Stomatology, Weifang Yidu Central Hospital, Qingzhou 262500, Shandong, China
  • Online:2020-02-10 Published:2022-09-27

PDF (PC)

49

Abstract: Objective To investigate the early expression of Frizzled 4 in peri-implant tissues of hyperlipidemic rats. Methods A total of 48 adult male SD rats were randomly divided into two groups, with 24 rats in either group. Rats in the experimental group were fed with high fat diet, while rats in the control group were fed with common diet for 8 weeks. Implants 1.5 mm wide and 2.5 mm long were implanted into the metaphyseal end of femur. The rats were sacrificed on the 1st, 3rd, 5th and 28th days after operation to obtain bone tissues around the implant. The tissues were scanned with Inveon MM CT and 1 mm area around the implant was set as the area of interest(ROI). Three-dimensional reconstruction was performed in ROI. Bone trabecular parameters were analyzed, including trabecular volume/ROI volume(BV/TV), trabecular thickness(Tb.Th), trabecular number(Tb.N)and trabecular space(Tb.S). The gene expressions of osteoblasts, osteoclast differentiation markers Runt-related transcription factor 2(RUNX2), cathepsin K(CTSK)and Frizzled 4 were detected with RT-PCR. The protein expression of Frizzled 4 was determined with Western 山 东 大 学 学 报 (医 学 版)58卷2期 -李源,等.卷曲受体蛋白4在高脂血症大鼠种植体周围组织中的早期表达 \=- blotting. Results There were no significant differences in BV/TV, Tb.S, Tb.Th and Tb.N between the two groups on the 1st and 3rd day of implantation(P>0.05). On the 5th and 28th days of implantation, the experimental group had lower BV/TV, Tb.Th and Tb.N but higher Tb.S than the control group(P<0.05). On the 1st, 3rd, 5th and 28th days of implantation, the experimental group had lower mRNA expression of Runx2 but higher mRNA expression of CTSK than the control group(P<0.05). In the control group, the mRNA and protein expressions of Frizzled 4 increased significantly on the 3rd day of implantation, and decreased on the 5th and 28th days, but were still higher than those in the experimental group(P<0.05). In the experimental group, both mRNA and protein expressions of Frizzled 4 decreased significantly on the 3rd day of implantation, but increased on the 5th and 28th days(P<0.05). In the control group, the protein expression of Wnt/β-catenin increased significantly on the 3rd day of implantation, and decreased on the 5th and 28th days, but was still higher than that in the experimental group(P<0.05). In the experimental group, the expression of Wnt/β-catenin increased significantly on the 3rd day of implantation, but decreased on the 5th and 28th days(P<0.05). Conclusion Hyperlipidemia can inhibit early osseointegration of implants, which may be related to the down-regulation of Frizzled 4 expression. The exact mechanism needs further research.

Key words: Frizzled 4, Hyperlipidemia, Implants, Osteointegration

CLC Number: 

  • R782
[1] Antonoglou GN, Stavropoulos A, Samara MD, et al. Clinical performance of dental implants following sinus floor augmentation: a systematic review and meta-analysis of clinical trials with at least 3 years of follow-up [J]. Int J Oral Maxillofac Implants, 2018, 33(3): 45-65.
[2] Ragucci GM, Elnayef B, Su(~overa)rez-L(~overo)pez Del Amo F, et al. Influence of exposing dental implants into the sinus cavity on survival and complications rate: a systematic review[J]. Int J Implant Dent, 2019, 5(1): 6-13.
[3] Savvidis C, Tournis S, Dede AD. Obesity and bone metabolism [J]. Hormones(Athens), 2018, 17(2): 205-217.
[4] Keuroghlian A, Barroso ADV, Kirikian G, et al. The effects of hyperlipidemia on implant osseointegration in the mouse femur [J]. J Oral Implantol, 2015, 41(2): 7-11.
[5] Insua A, Monje A, Wang H, et al. Basis of bone metabolism around dental implants during osseointegration and peri-implant bone loss [J]. J Biomed Mater Res A, 2017,105(7): 2075-2089.
[6] Strakova K, Matricon P, Yokota C, et al. The tyrosine Y250(2.39)in Frizzled 4 defines a conserved motif important for structural integrity of the receptor and recruitment of Disheveled [J]. Cellular Signaling, 2017, 38: 85-96. doi: 10.1016/j.cellsig.2017.06.018.
[7] Liu F, Wang Z, Liu F, et al. MicroRNA-29a-3p enhances dental implant osseointegration of hyperlipidemic rats via suppressing dishevelled 2 and frizzled 4 [J]. Cell Biosci, 2018, 8: 55. doi:10.1186/s13578-018-0254-y.
[8] Ji H, Liu Y, He F, et al. LC-MS based urinary metabolomics study of the intervention effect of aloe-emodin on hyperlipidemia rats [J]. J Pharm Biomed Anal, 2018, 156(1): 104-115.
[9] Parwin A, Najmi AK, Ismail MV, et al. Protective effects of alendronate in Triton X-100-induced hyperlipidemia in rats [J]. Turk J Gastroenterol, 2019, 30(6): 557-564.
[10] Ma XY, Wen XX, Yang XJ, et al. Ophiopogonin D improves osteointegration of titanium alloy implants under diabetic conditions by inhibition of ROS overproduction via Wnt/β-catenin signaling pathway [J]. Biochimie, 2018, 152(1): 31-42.
[11] Magán-Fernández A, Papay-Ramírez L, Tomás J, et al. Association of simvastatin and hyperlipidemia with periodontal status and bone metabolism markers [J]. J Periodontol, 2015, 85(10): 1408-1415.
[12] Shibamoto A, Ogawa T, Duyck J, et al. Effect of high-frequency loading and parathyroid hormone administration on peri-implant bone healing and osseointegration [J]. Int J Oral Sci, 2018, 10(1): 6-13.
[13] López-Gómez JJ,Izaola-Jauregui O,Primo-Martín D, et al. Effect of weight loss on bone metabolism in postmenopausal obese women with osteoarthritis [J]. Obes Res Clin Pract, 2019, 13(4): 378-384.
[14] Marozik P, Alekna V, Rudenko E, et al. Bone metabolism genes variation and response to bisphosphonate treatment in women with postmenopausal osteoporosis [J]. PLoS One, 2019, 14(8): 221-226.
[15] Shigenobu K, Hashimoto T, Kanayama M, et al. The efficacy of osteoporotic treatment in patients with new spinal vertebral compression fracture pain, ADL, QOL, bone metabolism and fracture-healing-In comparison with weekly teriparatide with bisphosphonate [J]. Bone Reports, 2019, 11(6): 100-106.
[16] Yao RH, Nishii K,Kito T, et al. A novel device to prevent osteoporosis by promoting bone metabolism using a newly developed double-loading stimulation with vibration and shaking [J]. Okajimas Folia Anat Jpn, 2019, 96(1): 13-21.
[17] Zhang Y, Zhou L, Li S, et al. Impacts of lead exposure and chelation therapy on bone metabolism during different developmental stages of rats [J]. Ecotoxicol Environ Saf, 2019, 183(14): 104-113.
[18] I ngvild KBH,Stephen H,Monica CK,et al. Bone metabolism, bone mineral density and low-energy fractures 10 years after Roux-en-Y gastric bypass [J]. Bone, 2019, 127(6): 436-445.
[19] Lee S, Le NH, Kang D. Melatonin alleviates oxidative stress-inhibited osteogenesis of human bone marrow-derived mesenchymal stem cells through AMPK activation [J]. Int J Med Sci, 2018, 15(1): 1083-1091.
[20] Zhuang Y, Mao JQ, Yu M, et al. Hyperlipidemia induces vascular smooth muscle cell proliferation involving Wnt/β-catenin signaling [J]. Cell Biol Int, 2015, 40(2): 121-130.
[21] Song HB, Jiang Y, Liu JX, et al. Stimulation of osteogenic differentiation in bone marrow stromal cells via Wnt/β-catenin pathway by Qili Jiegu-containing serum [J]. Biomed Pharmacother, 2018, 103(1): 1664-1668.
[22] Bisson SK, Ung RV, Mac-Way F. Role of the Wnt/β-catenin pathway in renal osteodystrophy [J]. Int J Endocrinol, 2018, 16(5): 1-8.
[23] Long H, Sun B, Cheng L, et al. miR-139-5p represses BMSC osteogenesis via targeting Wnt/β-catenin signaling pathway [J]. DNA Cell Biol, 2017, 36(8): 715-724.
[24] Fu HD, Wang BK, Wan ZQ, et al. Wnt5a mediated canonical Wnt signaling pathway activation in orthodontic tooth movement: possible role in the tension force-induced bone formation [J]. J Mol Histol, 2016, 47(5): 455-466.
[25] Cédric L, Aline M, Marie-Thérèse L, et al. Fat and sucrose intake induces obesity-related bone metabolism disturbances: kinetic and reversibility studies in growing and adult rats [J]. J Bone Miner Res, 2016, 31(1): 98-115.
[1] YUAN Mengqi, HUO Fenglei, REN Huiping, GUO Qiushuang, LAN Jing. Effects of Sdccag3 on the osseointegration in hyperlipidemia rats through Wnt pathway [J]. Journal of Shandong University (Health Sciences), 2022, 60(7): 66-73.
[2] LONG Yuhan, LUO Xia, JIANG Jie. Abnormal hyperlipidemia during pregnancy: a report of 2 cases and literature review [J]. Journal of Shandong University (Health Sciences), 2021, 59(3): 103-106.
[3] ZHANG Guang, WANG Guangyin, WU Hongyan, ZHANG Hongyu, WANG Tingting, LI Jiqing, LI Min, KANG Fengling, LIU Yanxun, XUE Fuzhong. A prediction model of hyperlipidemia risk based on the health management population [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(6): 72-76.
[4] RONG Chunli, YAO Wenjing, YANG Qian, LI Ruyi, CAO Dongping, LIU Meixia, PENG Yingxin. Clinical efficacy of atorvastatincalcium combined with ezetimibe in the treatment of coronary atherosclerosis with hyperlipidemia [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(11): 46-49.
[5] WEI Ying, SUN Yawen, GAO Youmei, XUE Fuzhong, YU Hongxia, ZHANG Chengqi. Relationships of dietary patterns with overweight, hypertension, hyperglycemia and hyperlipidemia in career women in Jinan [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(8): 102-107.
[6] DI Jing, SHI Xiaoting, LI Xue, LIN Zhiyong, SUN Zhe, DENG Fengying. Effect of parathyroid hormone1-34 on bone healing around dental implants in ostoeporotic rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(8): 22-26.
[7] ZHANG Min1，2, XU Gui-fa1. Effect of nonpharmacotherapy in decreasing the levels of serum lipid in simple hyperlipidemia patients [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(8): 117-119.
[8] GAO Dezong, ZHANG Qiang, LI Liang, LI Yuyang, YU Zhigang. Nipple preserving modified radical mastectomy and immediate breast  reconstruction with silicone prosthesis for breast cancer [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(4): 106-.
[9] . Role of apolipoprotein E gene polymorphism and plasma lipids in mild cognitive impairment [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(11): 103-107.
[10] LI Jianzhong, ZHAO Jun, GUO Guangqing， CHEN Qi, LIU Jinxia, ZHANG Yanguo
. Apolipoprotein E gene expression in peripheral blood monocyte and lipid analysis of hypertension patients [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(02): 50-52.
[11] WU Mao-hong,WANG Feng-yun,SUN Lei,DONG Ming,REN Jian-min. Relationship between post-prandial hyperlipidemia and carotid ather oscerosis and blood rheology in type 2 diabetic patients [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(5): 503-507.
[12] LIU Xiang-ju,GAO Hai-qing,BI Yi,SHEN Lin,YOU Bei-an. Grape seed proanthocyanidin extract combined with atorvastatin in prevention of atherosclerosis in rabbits [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(11): 1131-1134.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd