JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (10): 52-58.doi: 10.6040/j.issn.1671-7554.0.2017.049

Previous Articles     Next Articles

Expression and clinical significance of EFEMP2 in endometrial cancer tissues and cell

WANG Tiantian, CHEN Jie   

  1. Department of Maternal and Child Health, School of Public Health, Shandong University, Jinan 250012, Shandong, China
  • Received:2017-01-13 Online:2017-10-10 Published:2017-10-10

PDF (PC)

155

Abstract: Objective To investigate expression and the clinical significance of EGF containing fibulin-like extracellular matrix protein 2(EFEMP2)in endometrial cancer tissues and cell lines. Methods Immunohistochemical staining was performed to determine EFEMP2 expression in 200 endometrial carcinoma specimens and 40 normal endometrial tissue specimens. qRT-PCR, Western blotting and immunocytochemistry were performed to investigate EFEMP2 expression in normal endometrial cells compared with four kinds of endometrial carcinoma cell lines including KLE、HEC-1A、HEC-1B and Ishikawa. Growth curve assay and soft agar colony formation assay were used to investigate the proliferation of endometrial cancer cell lines. Transwell assays were used to analyze the invasion and migration of endometrial cancer cell lines. Results EFEMP2 was down-regulated in endometrial cancer tissues and cells compared with normal endometrial tissues and cells(P<0.001), respectively. EFEMP2 low expression was significantly related with high stage, low differentiation and positive lymph node status of endometrial cancer(P<0.05). KLE and HEC-1A cells had higher proliferation, invasion and metastasis abilities compared to HEC-1B and Ishikawa cells(P<0.001). Conclusion EFEMP2 expression was significantly related with tumor stage, tissue differentiation and lymph node status of endometrial cancer. It is possible that EFEMP2 inhibits endometrial cancer cell invasion and migration.

Key words: Proliferation, Invasion, EFEMP2, Endometrial cancer, Migration

CLC Number: 

  • R574
[1] 刘海霞. 热休克因子1在子宫内膜癌侵袭转移中的作用研究[D]. 西安: 第四军医大学, 2014.
[2] Jin C, Liang R. miR-205 promotes epithelial-mesenchymal transition by targeting AKT signaling in endometrial cancer cells[J]. J Obstet Gynaecol Res, 2015, 41(10): 1653-1660.
[3] Kito M, Motoyama S, Fujita K, et al. CRP 1846C>T genetic polymorphism is associated with lymph node metastasis and/or severe lymphatic invasion in endometrial cancer[J]. Tohoku J Exp Med, 2015, 237(1): 25-30.
[4] Bakkum-Gamez JN, Mariani A, Dowdy SC, et al. Efficacy of contemporary chemotherapy in stage IIIC endometrial cancer: a histologic dichotomy[J]. Gynecol Oncol, 2014, 132(3): 578-584.
[5] Chaffer CL, Weinberg RA. A perspective on cancer cell metastasis[J]. Science, 2011, 331(6024): 1559-1564.
[6] Chen J, Zhang J, Liu X, et al. Overexpression of fibulin-4 is associated with tumor progression and poor prognosis in patients with cervical carcinoma[J]. Oncol Rep, 2014, 31(6): 2601-2610.
[7] 郑燃, 李博, 欧阳玲, 等. 盆腔器官脱垂患者子宫主韧带和阴道前壁中Fibulin-4蛋白的表达及意义[J]. 中国医科大学学报, 2012, 41(3): 231-233. ZHENG Ran, LI Bo, OUYANG Ling, et al. Expression and significance of fibulin-4 in cardinal ligament of uterus and anterior vaginal wall tissue of pelvic organ prolapse[J]. Journal of China Medical University, 2012, 41(3):231-233.
[8] Wlazlinski A, Engers R, Hoffmann MJ, et al. Downregulation of severa fibulin genes in prostate cancer [J]. Prostate, 2007, 67(16): 1770-1780.
[9] Berk DR, Bentley DD, Bayliss SJ, et al. Cutis laxa: a review[J]. J Am Acad Dermatol, 2012, 66(5):842. e1-17.doi:10.1016/j.jaad.2011.01.004.
[10] Huang JB, Yamashiro Y, Papke CL, et al. Angiotensin-converting enzyme-induced activation of local angiotensin signaling is required for ascending aortic aneurysms in fibulin-4-deficient mice[J]. Sci Transl Med, 2013, 5(183):183ra58,1-11. doi: 10.1126/scitranslmed.3005025.
[11] Xiang Y, Sekine T, Nakamura H, et al. Fibulin-4 is a target of autoimmunity predominantly in patients with osteoarthritis[J]. J Immunol, 2006, 176(5): 3196-3204.
[12] Gallagher WM, Greene LM, Ryan MP, et al. Human fibulin-4: analysis of its biosynthetic processing and mRNA expression in normal and tumour tissues[J]. FEBS Lett, 2001, 489(1): 59-66.
[13] 侯勇丽. 核因子kappaBp65蛋白及ENA-78在子宫内膜异位症发病中的作用研究[D]. 太原: 山西医科大学, 2009.
[14] 刘晓燕, 张捷, 陈洁. 高迁移率族蛋白B1在卵巢癌组织及单克隆细胞亚系中的表达及其临床意义[J]. 山东大学学报(医学版), 2011, 49(12): 123-127, 131. LIU Xiaoyan, ZHANG Jie, CHEN Jie. Expression and clinical significance of high mobility group box l in ovarian cancer tissues and monoclonal cell sub-clones[J]. Journal of Shandong University(Health Sciences), 2011, 49(12): 123-127, 131.
[15] Chen J, LIU Z, Fang S, et al. Fibulin-4 is associated with tumor progression and a poor prognosis in ovarian carcinomas[J]. BMC Cancer, 2015, 15(4):91.doi 10.1186/s12885-015-1100-9.
[16] Han G, Wu DW, Yang YA, et al. CrkL meditates CCL20/CCR6-induced EMT in gastric cancer[J]. Cytokine, 2015, 76(2): 163-169.
[17] Gallagher WM, Currid CA, Whelan LC. Fibulins and cancer: friend or foe?[J]. Trends Mol Med, 2005, 11(7): 336-340.
[18] Obaya AJ, Rua S, Moncadapazos A, et al. The dual role of fibulins in tumorigenesis[J]. Cancer Lett, 2012, 325(2): 132-138.
[19] Papke CL, Yanagisawa H. Fibulin-4 and fibulin-5 in elastogenesis and beyond: Insights from mouse and human studies[J]. Matrix Biol, 2014, 37:142-149. doi:10.1016/j.matbio.2014.02.004.
[20] Chen LX, Sun BC, Zhang SW, et al. Influence of microenvironments on microcirculation patterns and tumor invasion-related protein expression in melanoma[J]. Oncol Rep, 2009, 21(4): 917-923.
[21] Li RZ, Wang LM. Fibulin-4 is a novel Wnt/beta-Catenin pathway activator in human osteosarcoma[J]. Biochem Biophys Res Commun, 2016, 474(4): 730-735.
[22] Wang L, Chen QX, Chen ZB, et al. EFEMP2 is upregulated in gliomas and promotes glioma cell proliferation and invasion[J]. Int J Clin Exp Pathol, 2015, 8(9): 10385-10393.
[1] WANG Wei, WANG Yi-Feng, ZHANG Ling-Mei, LIN Qiong-Yan, HUANG Ju. umorigenicity and invasion of side population in human ovarian epithelial cancer OVCAR3 cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 8-11.
[2] WANG Xiao-Lei, ZHANG Hai-Thao, ZHANG Hui, GUO Cheng-Hao. Protective effects of shuxuening injecta on vascular  endothelial  cell injury in vitro due to iodine excesses [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2209, 47(6): 38-.
[3] SUN Yifeng, GAO Yu, LIANG Yongyuan, GAO Yang. Expression of CPLX2 and its in vitro effects on the proliferation migration and invasion of hepatocellular carcinoma cells [J]. Journal of Shandong University (Health Sciences), 2020, 1(9): 34-39.
[4] ZHANG Baowen, LEI Xiangli, LI Jinna, LUO Xiangjun, ZOU Rong. miR-21-5p targeted TIMP3 to inhibit proliferation and extracellular matrix accumulation of mesangial cells in Type II diabetic nephropathy mice [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 7-14.
[5] MA Qingyuan, PU Peidong, HAN Fei, WANG Chao, ZHU Zhoujun, WANG Weishan, SHI Chenhui. Effect of miR-27b-3p regulating SMAD1 on osteosarcoma cell proliferation, migration and invasion [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 32-37.
[6] LI Ning, LI Juan, XIE Yan, LI Peilong, WANG Yunshan, DU Lutao, WANG Chuanxin. Expression of LncRNA AL109955.1 in 80 cases of colorectal cancer and its effect on cell proliferation, migration and invasion [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 38-46.
[7] WANG Kaimin, TAN Juanjuan, YAN Zhiqiang, LI Zhiqiang. Effects of Sirtuin on hypoxia-induced proliferation of human umbilical cord-derived mesenchymal stem cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(7): 23-30.
[8] SHI Peikun, ZENG Beini, WU Weifang, HU Xiaoyan, MA Tianjia, ZHOU Yabin. Expression and role of Keap1 in renal cell carcinoma [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 94-99.
[9] SUN Zeyu, CHEN Ying, LIN Jiaxiang, LIU Juan, ZHAO Weiming. Influence of inositol polyphosphate-4-phosphatase type II on the proliferation and apoptosis of cervical cancer cells [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(11): 1-6.
[10] LU Heng, LIU Yanguo, LI Shuguang, CHEN Xiaokang, TIAN Qi, YI Cuihua, WANG Xiuwen. Regulation of epithelial ovarian carcinoma SKOV-3 cell migration by YKL-40 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(1): 33-38.
[11] ZHAO Lu, JIAO Jun, ZHANG Teng, JIAO Xinlin, CUI Baoxia. Expression of LXRα in ovarian carcinoma and effects of T0901317 on SKOV3 cell line [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(1): 49-53.
[12] TIAN Siqi, LIU Riqiang, YANG Ning, WEI Wei, YANG Huawei. Effect of expression of ribosomal protein S6 Kinase 4 variants on the proliferation of breast cancer cell MDA-MB-231 [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(9): 32-36.
[13] WANG Wei, CAO Yushan, SUN Daquan, HUANG Xiaoqiong, XU Guoqiang. Role of human TIMP-2 protein in the migration and invasion of hepatocellular carcinoma cell [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(7): 11-17.
[14] HE Wubin, SU Rongjian. Pulsatilla saponin D combined with sorafenib against hepatocellular carcinoma cell invasion and metastasis [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(7): 18-22.
[15] MA Xue, ZHANG Bin, HAN Chunyao, LIU Mingyuan, HAO Lijing, GE Shuqing, XUE Zhongyuan. Down-regulation of α-catulin inhibits the invasion and migration of human tongue squamous cancer cell line Tscca in vitro [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(6): 12-15.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd