JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (3): 50-55.doi: 10.6040/j.issn.1671-7554.0.2014.690

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Proliferative effect of TGF-β1 phage model peptide on fibroblasts

LIU Zhenzhong1, JIANG Duyin1, WANG Wei1, ZONG Xianlei2, ZHANG Jixun1, LIU Lei1   

  1. 1. Department of Plastic Surgery, the Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    2. Sixteenth Department of Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Beijing 100144, China
  • Received:2014-10-13 Revised:2015-01-14 Online:2015-03-10 Published:2015-03-10

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Abstract: Objective To isolate transforming growth factor-beta1 (TGF-β1) phage model peptides from phage display 12-mer peptide library, and to evaluate their proliferative effect on normal skin fibroblasts. Methods Phage 12-mer display peptide library was screened with monoclonal anti-human TGF-β1 to get specific phage model peptides. The model with sequence most similar to TGF-β1 was chosen and 4 groups were enrolled:negative control group, MP13 control group, TGF-β1 control group, and phage model peptide group. MTT assay was used for the quantitative determination of cellular proliferation. Immunofluorescence assay was employed to show the affinity of the model peptide on skin fibroblasts. Quantitative Real-time PCR analysis was carried out to detect the expressions of I collagen (COL1), III collagen (COL3) and connective tissue growth factor (CTGF). Results A total of 10 phage model peptides were obtained. The first one with sequence most similar to TGF-β1 was chosen. The MTT results showed that the phage model peptide could promote fibroblast proliferation (P<0.05). Immunofluorescence assay revealed that the model peptide on phages rather than phages could bind to the fibroblasts (P<0.05). The Real-time PCR analysis suggested that the relative expressions of COL1 and COL3 significantly increased in the model group than in the negative control group and M13 group (P<0.05). The expression of CTGF mRNA increased dramatically after treatment withphage model peptide, and reached the peak at 2 d (P<0.05). Conclusion The phage model peptide isolated from phage 12-mer display peptide library can promote the proliferation of normal skin fibroblasts by regulating the expression of CTGF.

Key words: Peptide library, Phage display, Transforming growth factor-beta1, Fibroblasts, Proliferation

CLC Number: 

  • R622
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