JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (3): 56-61.doi: 10.6040/j.issn.1671-7554.0.2014.744

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Changes of Runx2 and Osterix expression in type 1 diabetic rats and the intervention effect of zoledronic acid

ZHAO Xu, CUI Min, YU Lingzhi, ZHANG Na, CAO Luning   

  1. Department of Pain Management, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, Shandong, China
  • Received:2014-10-27 Revised:2015-02-28 Online:2015-03-10 Published:2015-03-10

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Abstract: Objective To observe the levels of serum insulin and osteocalcin, osteoblast specific transcription factor Runx2 and Osterix (OSX) gene in type 1 diabetic rats, and the intervention effect of zoledronic acid. Methods A total of 170 Wistar rats were randomly divided into two groups:40 rats were enrolled into the control group the other animals were injected with streptozotocin (60 mg/kg) to establish type 1 diabetic models. After that, 120 rats in this group were randomly divided into the model group (n=40), prevention group (n=40) and treatment group (n=40). Rats in the prevention group and treatment group received a bolus injection of zoledronic acid (0.1 mg/kg) at the onset of diabetes and 2 weeks later, respectively. The serum INS and OC were detected with ELISA. Expressions of Runx2 and OSX mRNA in the right femur were detected with RT-PCR. Results ELISA results showed that the serum INS in the model group, prevention group and treatment group was significantly reduced compared with that in the control group (P<0.05). The serum OC was lower in the model group that in the control group (P<0.05), while higher in the prevention group than in the control group and model group (P<0.05). At week 12, serum OC was higher in the treatment group than in the prevention group (P<0.05). RT-PCR tests showed that the expressions of Runx2 andOSX mRNA were significantly lower in the model group than in the control group (P<0.05), while remarkably higher in the prevention group and treatment group than in the control group and model group (P<0.05). The upregulation was higher in the prevention group than in the model group. Conclusion Expressions of Runx2 and OSX mRNA were significantly decreased in the bone of type 1 diabetic rat models. Prophylactic use of zoledronic acid can improve the levels of serum OC, and reverse the decreasing expression of Runx2 and OSX mRNA, thus promoting bone formation.

Key words: Insulin, Osteocalcin, Runx2, Type 1 diabetes, Osterix, Zoledronic acid

CLC Number: 

  • R589
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