JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (10): 11-15.doi: 10.6040/j.issn.1671-7554.0.2015.230

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Radioprotective effects of valproic acid on normal brain tissues of rats

ZHANG Wei, ZHOU Yong, NIU Junjie, XU Ying, HOU Huaying, JIANG Yuhua   

  1. Cancer Prevention & Control Center, Second Hospital of Shandong University, Jinan 250033, Shandong, China
  • Received:2015-03-03 Online:2015-10-10 Published:2015-10-10

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Abstract: Objective To investigate the radioprotective effects of valproic acid (VPA), an antiepileptic drug, on the normal brain tissues of rats. Methods A total of 48 male Wistar rats were randomized into VPA group, radiotherapy group, combined group, control group, with 12 animals in each group. VPA group received sham irradiation plus VPA 150 mg/kg; radiotherapy group underwent X-ray irradiation plus physiological saline 150 mg/kg; combined group received X-ray irradiation plus VPA 150 mg/kg; control group received sham irradiation plus physiological saline 150 mg/kg. All treatments were administered twice a day for 5 days. Cell apoptosis of the brain tissues was determined with immunohistochemistry using an antibody for protein Caspase-3 24 hours after irradiation. Then the changes of body weight within 2 weeks starting from the first injection of VPA were recorded. The neuron nucleus changes were observed with transmission electron microscope (TEM) 6 months after radiotherapy. Results Immunohistochemistry results demonstrated that Caspase-3 was significantly increased in radiotherapy groups compared to the combined group. The average weight of rats in radiotherapy group decreased markedly compared with the combined group (P<0.05). TEM observation showed that neuron nuclear membrane surface was conveoconcave and irregular, or even impaired in radiotherapy group; it was a little conveoconcave in combined group, but no impairment was observed; no apparent abnormalities were observed in control group and VPA group. Conclusion VPA could protect normal brain tissues of rats by inhibiting the apoptosis of normal nerve cells arising from radiation.

Key words: Histone deacetylase inhibitors, Valproic acid, Radioprotection, Apoptosis, Fractionated radiotherapy

CLC Number: 

  • R815.2
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