JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (1): 10-15.doi: 10.6040/j.issn.1671-7554.0.2014.715

Previous Articles     Next Articles

Hydrogen-rich water alleviates ischemia-reperfusion injury in mice with fatty liver

LI Shaowei1,2, LIU Zongzheng1,2, LIU Chunxia1,2, ZHANG Yanru1,2, ZHOU Huanmin1,2   

  1. 1. College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, Inner Mongolia, China;
    2. Inner Mongolia Key Laboratory of Biomanufacture, Hohhot 010018, Inner Mongolia, China
  • Received:2014-10-16 Revised:2014-12-17 Published:2015-01-10

PDF (PC)

221

Abstract: Objective To explore the protective effect of hydrogen-rich water on liver ischemia-reperfusion (I/R) in mice models with fatty liver and the possible mechanism. Methods A total of 30 C57BL/6 mice were randomly divided into 3 groups: sham-operated group (SO), control group (mice with steatotic liver perfused with water before ischemia induction) and hydrogen-rich water group (mice with steatotic liver perfused with hydrogen-rich water before ischemia reperfusion). Six hours after reperfusion, the mice were sacrificed, and serum and liver samples were collected. The ALT and AST levels in serum were determined and liver histological damage was evaluated. The infiltration of macrophages was detected. The mRNA expressions of TNF-α, IL-6, and OPN mRNA were assayed using real-time reverse transcription PCR. The expression of activated Caspase-3 was checked with Western blotting. Results Compared with control group, 6 hours following reperfusion, mice in hydrogen-rich water group exhibited lower levels of ALT and AST (P<0.05), lower expression of inflammatory cytokines and Caspase-3, milder histological damage, and less infiltration of macrophages (P<0.05). Conclusion Hydrogen-rich water given by gavage can alleviate steatotic liver ischemia-reperfusion injury by inhibiting oxidant stress, inflammatory response and apoptosis induced by reperfusion.

Key words: Nonalcoholic fatty liver, Hydrogen rich water, Reperfusion injury, Oxidant stress, Inflammatory

CLC Number: 

  • R575
[1] Bhala N, Angulo P, van der Poorten D, et al. The natural history of nonalcoholic fatty liver disease with advanced fibrosis or cirrhosis: an international collaborative study[J]. Hepatology, 2011, 54(4):1208-1216.
[2] Bhala N, Usherwood T, George J. Non-alcoholic fatty liver disease [J]. BMJ, 2009, 339: b2474. doi: 10.1136/bmj.b2474.
[3] Yang YY, Lee PC, Huang YT, et al. Involvement of the HIF-1alpha and Wnt/beta-catenin pathways in the protective effects of losartan on fatty liver graft with ischaemia/reperfusion injury[J]. Clin Sci (Lond), 2014, 126(2):163-174.
[4] Ohsawa I, Ishikawa M, Takahashi K, et al. Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals[J]. Nat Med, 2007, 13(6):688-694.
[5] Sun H, Chen L, Zhou W, et al. The protective role of hydrogen-rich saline in experimental liver injury in mice[J]. J Hepatol, 2011, 54(3):471-480.
[6] Theruvath TP, Ramshesh VK, Zhong Z, et al. Icam-1 upregulation in ethanol-induced Fatty murine livers promotes injury and sinusoidal leukocyte adherence after transplantation[J]. HPB Surg, 2012, 2012:480893. doi:10.1155/2012/480893.
[7] 游玉霞, 李建军, 李玉洁, 等. 富氢水对大鼠视神经夹伤模型视网膜神经节细胞保护作用的实验研究[J]. 眼科, 2014, 23(2):107-110. YOU Yuxia, LI Jianjun, LI Yujie, et al. Neuroprotective effects of saturated hydrogen water on retinal ganglion cells in rat optic nerve crush model[J]. Ophthalmol CHN, 2014, 23(2):107-110.
[8] 曾葵, 黄瀚, 江晓琴, 等. 氢气对大鼠肾脏缺血再灌注损伤的保护作用[J]. 四川大学学报:医学版, 2014, 45(1):39-42. ZENG Kui, HUANG Han, JIANG Xiaoqin, et al. Protective Effects of Hydrogen on renal Ischemia/Reperfusion Injury in Rat[J]. J Sichuan Univ: Med Sci Edi, 2014, 45(1):39-42.
[9] 刘琪, 王大为, 陈兴, 等. 氢气对吸入性损伤大鼠急性肺损伤的保护作用及量效研究[J].天津医科大学学报, 2013, 19(6):445-448. LIU Qi, WANG Dawei, CHEN Xing, et al. Protective effects of hydrogen gas on acute lung injury induced by smoke inhalation in rats and its dose effect relationship[J]. Journal of Tianjin Medical University, 2013, 19(6):445-448.
[10] Kudo H, Yata Y, Takahara T, et al. Telmisartan attenuates progression of steatohepatitis in mice: role of hepatic macrophage infiltration and effects on adipose tissue[J]. Liver Int, 2009, 29(7):988-996.
[11] Teramoto K, Bowers J, Kruskal JB, et al. Hepatic microcirculatory changes after reperfusion in fatty and normal liver-transplantation in the rat[J].Transplantation, 1993, 56(5):1076-1082.
[12] Diehl AM. Nonalcoholic steatosis and steatohepatitis-IV. Nonalcoholic fatty liver disease abnormalities in macrophage function and cytokines[J]. Am J Physiol Gastrointest Liver Physiol, 2002, 282(1):G1-G5.
[13] Zhang ZX, Shek K, Wang SA, et al. Osteopontin expressed in tubular epithelial cells regulates NK cell-mediated kidney ischemia reperfusion injury[J]. J Immunol, 2010, 185(2):967-973.
[14] Jiang M, Li J, Peng Q, et al. Neuroprotective effects of bilobalide on cerebral ischemia and reperfusion injury are associated with inhibition of pro-inflammatory mediator production and down-regulation of JNK1/2 and p38 MAPK activation[J]. J Neuroinflammation, 2014, 11(1):167.
[15] Liu X, Mei Z, Qian J, et al. Puerarin partly counteracts the inflammatory response after cerebral ischemia/reperfusion via activating the cholinergic anti-inflammatory pathway[J]. Neural Regen Res, 2013, 8(34):3203-3215.
[16] Canbay A, Feldstein AE, Higuchi H, et al. Kupffer cell engulfment of apoptotic bodies stimulates death ligand and cytokine expression[J]. Hepatology, 2003, 38(5):1188-1198.
[17] Nace GW, Huang H, Klune JR, et al. Cellular-specific role of toll-like receptor 4 in hepatic ischemia-reperfusion injury in mice[J]. Hepatology, 2013, 58(1):374-387.
[18] Qiu Z, Zhou D, Sun D. Effects of human umbilical cord mesenchymal stem cells on renal ischaemia-reperfusion injury in rats[J]. Int Braz Urol, 2014, 40(4):553-561.
[19] Serviddio G, Bellanti F, Tamborra R, et al. Uncoupling protein-2 (UCP2) induces mitochondrial proton leak and increases susceptibility of non-alcoholic steatohepatitis (NASH) liver to ischaemia-reperfusion injury[J]. Gut, 2008, 57(7):957-965.
[20] Baskin-Bey ES, Canbay A, Bronk SF, et al. Cathepsin B inactivation attenuates hepatocyte apoptosis and liver damage in steatotic livers after cold ischemia-warm reperfusion injury [J]. Am J Physiol Gastroint Liver Physiol, 2005, 288(2):G396-G402.
[21] Kamata H, Honda S, Maeda S, et al. Reactive oxygen species promote TNF alpha-induced death and sustained JNK activation by inhibiting MAP kinase phosphatases[J]. Cell, 2005, 120(5):649-661.
[1] LONG Tingting, XIE Ming, ZHOU Lu, ZHU Junde. Effect of Noggin protein on learning and memory abilities and the dentate gyrus structure after cerebral ischemia reperfusion injury in mice [J]. Journal of Shandong University (Health Sciences), 2020, 1(7): 15-23.
[2] LIU Fang, QIAO Lei, CHEN Wenchao, YANG Lina. Cardioprotective effects of allicin postconditioning on myocardial ischemia-reperfusion injury in rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(7): 6-11.
[3] JIANG Zheng, SHEN Zhenwei, ZHANG Guang, LI Runzi, CAO Jin, WANG Li, XUE Fuzhong, LIU Yanxun. Construction of a NAFLD screening tool based on health examination subjects [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(6): 114-118.
[4] LIU Xiaojuan, JIANG Zheng, KANG Fengling, ZHOU Miao, LIN Weiqiang, XUE Fuzhong. Association between neutrophil count and nonalcoholic fatty liver disease:a prospective cohort study [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(6): 119-123.
[5] ZHANG Luan, CHEN Ou, LUAN Yun, ZHU Xiaobo, CHEN Yuan, WANG Yibiao. Effects of gemigliptin on the therapeutic effect and inflammatory factors of monocrotaline-induced pulmonary arterial hypertension in rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(5): 19-22.
[6] YAN Songhe, GAO Lifen, ZHAO Wei, WANG Yan. Inhibition of human apical papilla cells on the activation of mononuclear cell THP-1 and its molecular mechanism [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 49-53.
[7] ZHANG Pengliang, ZHANG Longteng, LI Ailing, SHEN Tao, LOU Hongxiang, WANG Xiaoning. Isolation of Leptosphaerins H and I, two new xanthones from the endophytic fungus Leptosphaeria sp. and their anti-inflammatory activity [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(11): 27-31.
[8] LIANG Lining, ZHONG Xia, JI Xianfei, HU Haoran, ZHU Fangfang, CHEN Jibin,CHEN Qianqian, SHANG Deya. Protective effects of post-conditioning with nicorandil on myocardial, cerebral and renal ischemia-reperfusion injury following cardiac arrest in swine [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(10): 46-51.
[9] YANG Yanjun, LIANG Jing, LI Fang, DU Lingxi. Effect of halofuginone on macrophage polarization in endometriosis mouse [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(9): 26-31.
[10] LIN Dong, GUAN Qingbo. Association of serum testosterone level and non-alcoholic fatty liver disease in male patients with T2DM [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(7): 33-37.
[11] WANG Qingshi, CHEN Yunzhen, LIU Haichun, WU Wenliang, JIAO Guangjun, LI Xiaofeng, XU Daxia. Effects of inflammatory cytokines secreted by nucleus pulposus cells on proliferation and osteogenic ability of posterior longitudinal ligament fibroblasts [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(6): 22-26.
[12] LI Xin, WANG Gongming, WANG Hong, WANG Yan, ZHANG Ligong, ZHANG Le, LIU Bei, ZHANG Mengyuan. Inducible nitric oxide synthase contributes to the delayed cardioprotection of ischemic postconditioning in rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2016, 54(2): 16-20.
[13] LI Shaowei, LIU Zongzheng, LIU Chunxia, ZHANG Yanru, ZHOU Huanmin. Protective effect of grape seed proanthocyanidins in mouse fatty liver models suffering ischemia-reperfusion injury [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(9): 41-46.
[14] YANG Shujuan, SU Shili, DONG Baihua. Changes of vaginal microenvironment in 20 women after NuvaRing contraceptive ring placement [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(7): 58-60.
[15] LI Minghua, WANG Jiali, XU Feng, YUAN Qiuhuan, LIU Baoshan, PANG Jiaojiao, ZHANG Yun, CHEN Yuguo. Acute hyperglycemia exacerbates myocardial ischemia/reperfusion injury by inhibiting aldehyde dehydrogenase 2 activity in rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(5): 15-20.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Copyright 2018 © Editorial office of Journal of Shandong University (Health Sciences)
Supported by Beijing Magtech Co.Ltd