JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2015, Vol. 53 ›› Issue (1): 10-15.doi: 10.6040/j.issn.1671-7554.0.2014.715

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Hydrogen-rich water alleviates ischemia-reperfusion injury in mice with fatty liver

LI Shaowei1,2, LIU Zongzheng1,2, LIU Chunxia1,2, ZHANG Yanru1,2, ZHOU Huanmin1,2   

  1. 1. College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, Inner Mongolia, China;
    2. Inner Mongolia Key Laboratory of Biomanufacture, Hohhot 010018, Inner Mongolia, China
  • Received:2014-10-16 Revised:2014-12-17 Published:2015-01-10

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Abstract: Objective To explore the protective effect of hydrogen-rich water on liver ischemia-reperfusion (I/R) in mice models with fatty liver and the possible mechanism. Methods A total of 30 C57BL/6 mice were randomly divided into 3 groups: sham-operated group (SO), control group (mice with steatotic liver perfused with water before ischemia induction) and hydrogen-rich water group (mice with steatotic liver perfused with hydrogen-rich water before ischemia reperfusion). Six hours after reperfusion, the mice were sacrificed, and serum and liver samples were collected. The ALT and AST levels in serum were determined and liver histological damage was evaluated. The infiltration of macrophages was detected. The mRNA expressions of TNF-α, IL-6, and OPN mRNA were assayed using real-time reverse transcription PCR. The expression of activated Caspase-3 was checked with Western blotting. Results Compared with control group, 6 hours following reperfusion, mice in hydrogen-rich water group exhibited lower levels of ALT and AST (P<0.05), lower expression of inflammatory cytokines and Caspase-3, milder histological damage, and less infiltration of macrophages (P<0.05). Conclusion Hydrogen-rich water given by gavage can alleviate steatotic liver ischemia-reperfusion injury by inhibiting oxidant stress, inflammatory response and apoptosis induced by reperfusion.

Key words: Nonalcoholic fatty liver, Hydrogen rich water, Reperfusion injury, Oxidant stress, Inflammatory

CLC Number: 

  • R575
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