JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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Exploration of the protective role of antifreeze protein III on mouse embryo in vitrification

WEN Yan1, ZHAO Shuqin2, SHEN Yanjun1, CHAO Lan1, SONG Changzheng3, DENG Xiaohui1   

  1. 1. Infertility Center, Qilu Hospital of Shandong University, Jinan 250012， Shangdong,China;
    2. Infertility Center, Zaozhuang Maternal and Child Care Service Hospital, Zaozhuang 277100, Shangdong， China;
    3. Shandong Academy of Medical Sciences, Jinan 250062, Shangdong， China
  • Received:2013-12-10 Online:2014-05-10 Published:2014-05-10

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Abstract: Objective  To observe whether antifreeze protein III (AFPIII) is able to protect cytoskeleton microfilament and the potential development after recovery while being used in the vitrification of two-cell stage embryos. Methods   A total of 1 200 two-cell stage embryos were collected and randomly divided into 3 groups: fresh control group, vitrification group, and vitrification group with additional AFPIII (500ng/mL) group, with 400 embryos in each group. Half of the embryos in each group were used for blastocyst culture after recovery, and the left were used for immunofluorescent staining of the microfilament, to compare the recovery rate, the following blastocyst formation rate, and the rate of formal cytoskeleton microfilament. Results  The recovery rate in the vitrification group with additional AFPIII was higher than that of the vitrification group (P<0.05); the ratio of blastocyst formation rate and intact actin filaments were higher in the fresh control group and vitrification group with additional AFPIII, compared to that of the vitrification group (both P<0.05); there was no statistical difference between the fresh control group and vitrification group with additional AFPIII (P>0.05). Conclusion  AFPIII plays a protective role on mouse two-stage embryos in vitrification.

Key words: Antifreeze protein III; , Vitrification, Mouse, Embryo

CLC Number: 

  • R574
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