Journal of Shandong University (Health Sciences) ›› 2023, Vol. 61 ›› Issue (9): 10-18.doi: 10.6040/j.issn.1671-7554.0.2022.1289

• Preclinical Medicine • Previous Articles     Next Articles

Effects of Helicobacter pylori infection on m6A level in gastric cancer cells and its mechanism

SUN Jingguo1, ZHU Wenshuai1, LU Yi2, MA Xiaoli1, JIA Yanfei1   

  1. 1. Research Center of Basic Medicine, Jinan Central Hospital, Shandong University, Jinan 250010, Shandong, China;
    2. Research Center of Basic Medicine, Jinan Central Hospital, Shandong First Medicine University, Jinan 250010, Shandong, China
  • Received:2022-11-11 Published:2023-10-10

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Abstract: Objective To investigate the effects of Helicobacter pylori(HP)infection on the level of m6A in gastric cancer and the mechanism. Methods After gastric cancer cell line MGC-803 was co-cultured with HP strains NCTC 11637, NCTC 26695 and PM SS1 at MOI=100 for 8 hours, the m6A level was detected with dot-blot and ELISA. The expressions of METTL3 and METTL14 were detected with qRT-PCR and Western blotting, respectively. Relevant data were downloaded from the GEO database to analyze the relationship between m6A methyltransferase and clinicopathological parameters. The difference in the expression of METTL14 in gastric cancer and normal tissues from TCGA and GTEx databases was analyzed with GEPIA. Results HP upregulated the m6A level in gastric cancer cells. HP infection promoted METTL14 expression. METTL14 expression correlated with LAUREN typing and was higher in intestinal type gastric cancer. METTL14 expression was higher in gastric cancer tissues than in normal adjacent tissues. Conclusion HP infection increases the m6A level in gastric cancer cells, which may be caused by the upregulation of METTL14.

Key words: Helicobacter pylori, Gastric cancer, Methyltransferase-like protein 14, Methyltransferase-like protein 3, m6A

CLC Number: 

  • R735.2
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