Journal of Shandong University (Health Sciences) ›› 2023, Vol. 61 ›› Issue (2): 16-24.doi: 10.6040/j.issn.1671-7554.0.2022.0965

• 基础医学 • Previous Articles     Next Articles

Effects of Helicobacter pylori infection on glycolysis of gastric cancer cells

WANG Zan1,2, XU Xiaohan1, ZHANG Yu3, QU Yemin2, WANG Mingyi2, CHEN Ai1,2   

  1. 1. School of Medical Laboratory, Weifang Medical University, Weifang 261053, Shandong, China;
    2. Department of Central Lab, Weihai Municipal Hospital, Weihai 264200, Shandong, China;
    3. School of Laboratory Medicine, Dalian Medical University, Dalian 116000, Liaoning, China
  • Published:2023-02-17

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Abstract: Objective To explore the effects of Helicobacter pylori(H. pylori)on glycolysis metabolism of gastric cancer cells and the molecular mechanism. Methods After the glucose consumption rate and lactate production of gastric mucosal epithelial cells GES-1 and gastric cancer cells including MKN28, AGS, HGC27, MGC803, MKN45 and NCI-N87 were detected, GES-1, MKN28 and AGS were identified as the follow-up research objects. Untreated GES-1, MKN28 and AGS were set as controls, while they were co-cultured with H. pylori(H. pylori-HVS, H. pylori-26695, H. pylori-△cagA)to establish acute and chronic cell models. Glucose consumption, lactate production and glucose uptake were measured in all cell models. The protein levels of key enzymes in glucose metabolism were detected with Western blotting. Results Compared with normal gastric mucosal GES-1 cells, the gastric cancer cells(AGS, HGC27, MGC803, MKN45, NCI-N87)had significantly up-regulated glycolysis(P<0.001). Compared with the controls, the acute models had unchanged glucose uptake and lactate production(P>0.05), while chronic models had significantly increased glucose uptake and lactate production(P<0.001). The expression level of M2 isoform of pyruvate kinase(PKM2)was significantly up-regulated in the acute models, while that of M1 isoform of pyruvate kinase(PKM1)was down-regulated. Conclusion Chronic H. pylori infection can up-regulate glycolysis in gastric cancer cells, and the molecular mechanism may be related to PKM2, suggesting that PKM2 may be the target of H. pylori-mediated metabolic changes in gastric cancer.

Key words: Helicobacter pylori, Pyruvate kinase, Gastric cancer, Metabolism, Glycolysis

CLC Number: 

  • R735.2
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