Journal of Shandong University (Health Sciences) ›› 2023, Vol. 61 ›› Issue (7): 12-18.doi: 10.6040/j.issn.1671-7554.0.2022.1290

• 基础医学 • Previous Articles    

Expressions and clinical significance of DEC2 and HIF-2α in gastric cancer

FAN Rong1, LI Binbin1,2, MA Xiaoli1, WANG Yunshan1, JIA Yanfei1   

  1. 1. Research Center of Basic Medicine, Jinan Central Hospital, Shandong University, Jinan 250013, Shandong, China;
    2. Central Laboratory, Weihai Municipal Hospital, Weihai 264299, Shandong, China
  • Published:2023-07-04

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Abstract: Objective To investigate the expressions of differentiated embryonic chondrocyte gene 2(DEC2)and hypoxia inducible factor-2α(HIF-2α)and their relationship with the clinicopathological parameters of gastric cancer. Methods The gastric cancer cell lines MKN45 and HGC27 were divided into the experimental groups(hypoxia for 72 hours)and the control group(normoxic for 72 hours). The mRNA and protein expressions of DEC2 and HIF-2α were detected. The overexpression plasmid DEC2 was transfected into the cell lines to detect the protein expressions of DEC2 and HIF-2α. The expressions of DEC2 and HIF-2α in 60 cases of gastric cancer tissues and corresponding adjacent gastric mucosa tissues were detected with immunohistochemical staining, and their correlation was analyzed. Results After hypoxia treatment, the mRNA and protein expressions of DEC2 were significantly decreased in the experimental groups compared with the control groups, while the expressions of HIF-2α were increased. After overexpression of DEC2, the expression of HIF-2α decreased. The expression of DEC2 in gastric cancer tissues was significantly lower than that in adjacent gastric mucosa tissues(χ2=20.512, P<0.001), while the expression of HIF-2α was higher(χ2=11.172, P=0.001). The expressions of DEC2 and HIF-2α were significantly correlated with the depth of tumor invasion, lymph node metastasis and clinical stage, and the expressions of DEC2 and HIF-2α were negatively correlated with each other(P<0.001). Conclusion DEC2 is lowly expressed in gastric cancer cells and tissues, while HIF-2α is highly expressed. They are negatively correlated with other and closely related with the tumor development and metastasis.

Key words: Gastric cancer, Differentiated embryonic chondrocyte gene 2, Hypoxia inducible factor-2α, Hypoxia, Immunohistochemistry

CLC Number: 

  • R735.2
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