Journal of Shandong University (Health Sciences) ›› 2022, Vol. 60 ›› Issue (3): 24-28.doi: 10.6040/j.issn.1671-7554.0.2021.1264

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Effects of D-mannose and glucose on ulcerative colitis in mice

JIAN Tianzi1, CHEN Nuo2, LI Lixiang1, LI Yanqing1, LI Yan3   

  1. 1. Department of Gastroenterology, Qilu Hospital, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Immunology, School of Basic Medical Sciences, Shandong University, Jinan 250012, Shandong, China;
    3 Department of Pathogen Biology, School of Basic Medical Sciences, Shandong University, Jinan 250012, Shandong, China
  • Published:2022-03-09

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Abstract: Objective To investigate the effects of D-mannose and glucose on ulcerative colitis(UC)in mice induced by dextran sodium sulfate(DSS). Methods A total of 60 C57BL/6 mice were randomly divided into 6 groups: control group, D-mannose group, glucose group, DSS group, DSS + D-mannose group and DSS + glucose group, with 10 mice in each group. The mice were free to drink sterile water, 20% D-mannose, 20% glucose, 3.5% DSS, 3.5% DSS + 20% mannose or 3.5% DSS + 20% glucose for 7 days. The general condition, body mass, disease activity index(DAI), fecal characteristics and occult blood were observed and recorded every day. After 7 days, the mice were sacrificed, the colon was separated, the length was measured and histopathological observation was carried out. The spleen was resected to observe and measure the shape, length and body mass. Results The mice in the control group were generally in good condition, and the mice in the DSS group showed UC symptoms such as decreased body mass, diarrhea and macroscopic bloody stool. Compared with DSS group, D-mannose group had higher body mass ［(84.02±3.47)g vs(89.71±2.02)g, P<0.001］, significantly lower DAI score ［(3.10±0.77)vs(2.43±0.32), P=0.002］, significantly increased colon length ［(5.20±0.03)cm vs(6.87±0.19)cm, P<0.001］, and lower histopathological score ［(6.00±0.37)vs(4.25±0.28), P<0.001］. In DSS + glucose group, body mass decreased sharply(P<0.001), DAI score increased(P=0.001), intestinal bleeding aggravated(P<0.001), and histopathological score increased(P<0.001). Conclusion D-mannose can effectively improve the clinical symptoms of UC mice, while glucose aggravates the clinical symptoms.

Key words: Ulcerative colitis, Dextran sodium sulfate, D-mannose, Glucose, Mice

CLC Number: 

  • R574.1
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